Sodium Dedecyl Suphate Polyacrilamide Gel Electrophosis of Campylobacter coli

S.I. Smith, M.M. Ibrahim, V.N. Ezeobi, K.S. Oyedeji, K.A. Akinsinde, A.O Coker



Campylobacter coli were characterized using sodium dodecyl sulphate-polyacrilamide gel electrophoresis (SDS-PAGE). The isolates were obtained from the faeces of diarrhoeic children with the age range of 0 t 36 months attending paediatric clinic at the Lagos University Teaching Hospital (LUTH) and Obafemi Awolowo University Teaching Hospital Complex (OAUTHC), Ile-Ife, Nigeria. The 16 isolates studied were characterized into seven protein profiles based on their outer membrane proteins (OMPs). The glagella antigens of C. coli had the molecular weights of 24kDa and 84 kDa.

The SDS-PAGE proves a reliable and rapid technique for typing strains from sporadic cases.

(Af. J. of Clinical and Experimental Microbiology: 2002 3(1): 1-2)

The Discharging Ears In Adults In Ibadan, Nigeria Causative Agents And Antimicrobial Sensitivity Pattern

A.A. Oni, O.G.B. Nwaorgu, R.A. Bakare, M.O. Ogunkunle, R.A. Toki



In an attempt to study the microbiology of discharging ears, ear swabs were taken from 347 adult patient with discharging ears in the University College Hospital, Ibadan between March 1995 and February 1997. The presumptive diagnosis and indication for ear swabbing were chronic suppurative otitis media (67.1%), acute suppurative otitis media (14.4%) and otitis externa (18.2%). Using standard microbiological methods, 82.4% of the patients had microbes in their ears. These were identified as Pseudomonas aeruginosa (34.6%), Staphylococcus aerus (19.4%), Klebsiella species (17.4%) and Proteus species (12.5%). Others were Candida albicans and Aspergillus species.
Susceptibility result showed that ceftazidime, azithromycin, ceftriaxone, cefuroxime and gentimicin were active against majority of the bacterial isolates and are therefore recommended as first line drugs, while the quinolones should be kept as reserve durgs in the management of these conditions. In addition antifungal cream should be used as wick in dressing, as well as systemic metrondazole to take care of the anaerobes.(Af. J. of Clinical and Experimental Microbiology: 2002 3(1): 3-5)

Invitro antimicrobial susceptibility pattern of bacterial isolates from wound infections in university of Ilorin Teaching Hospital

S.S. Taiwo, A.B. Okesina, B.A Onile



The outcome of 532 wound swabs received from patients with wound infections in different units of the University of Ilorin Teaching Hospital Ilorin, Nigeria, over a one year period (July 2000 June 2001), and routinely procesed by Gram staining and culture in the Microbiology Laboratory, is reported. 444(83.5) of all samples cultured positive for bacterial pathogens while 88 (16.5%) were bacteriologically sterile. 272 swabs yielded single isolate while 172 yielded a mixture of two or more organisms. Staphylococcus aureus predominates (35.8%), followed by Pseudomonas spp (21.8%), Escherichia coli (15.3%), Klebsiella spp (13.4%), Proteus spp (5.6%), Coagulase Negative Staphylococci (3.1%), Streptococcus faecalis (2.8%), Streptococcs pyogenes (0.9%), Group B _-haemolytic Streptococci (0.9%), and Acinetobacter spp (0.3%).
Both Gram-positive and Gram-negative organisms demonstrated moderate to high in vitro sensitivity to Ofloxacin and Ciprofloxacin (sensitivity rate 70-94%). In vitro sensitivity to Cloxacillin, Erythromycin, Azithromycin and Ceftazidime by Gram positive organisms ranged beween 55 and 90% while Gentamicin, Ceftrazidime and Azithromycin equally demonstrated moderate to high inhibitory effect on Gram negative organisms including Pseudomonas spp. (sensitivity rate 55-90%).
The Fluoroquino lones are the favoured antimicrobial agents nowadays, as demonstrated in this study. In our environment however, a combination of Cloxacillin and Gentamicin is an effective empiric alternative when cost is considered and this combination can be used. The need for continuous antimicrobial monitoring of clinical isolates of wound infection for drug resistance, which is of paramount importance in the empiric selection of antibiotics, is emphasized.(Af. J. of Clinical and Experimental Microbiology: 2002 3(1): 6-10)