Sodium Dedecyl Suphate Polyacrilamide Gel Electrophosis of Campylobacter coli

S.I. Smith, M.M. Ibrahim, V.N. Ezeobi, K.S. Oyedeji, K.A. Akinsinde, A.O Coker



Campylobacter coli were characterized using sodium dodecyl sulphate-polyacrilamide gel electrophoresis (SDS-PAGE). The isolates were obtained from the faeces of diarrhoeic children with the age range of 0 t 36 months attending paediatric clinic at the Lagos University Teaching Hospital (LUTH) and Obafemi Awolowo University Teaching Hospital Complex (OAUTHC), Ile-Ife, Nigeria. The 16 isolates studied were characterized into seven protein profiles based on their outer membrane proteins (OMPs). The glagella antigens of C. coli had the molecular weights of 24kDa and 84 kDa.

The SDS-PAGE proves a reliable and rapid technique for typing strains from sporadic cases.

(Af. J. of Clinical and Experimental Microbiology: 2002 3(1): 1-2)