OSMOTIC FRAGILITY AND Na+ -K++ ATPase ACTIVITY OF ERYTHROCYTES OF HIV/AIDS PATIENTS

OAT Ebuehi, M Balogun, RA Audu, OE Idigbe

 

Abstract

A cross sectional study was carried out to investigate the osmotic fragility and Na+ -K+ ATPase activity of the erythrocytes of HIV/AIDS patients. Whole blood was taken from subjects at the Human Virology Laboratory of the Nigerian Institute of Medical Research. Subjects were judged suitable for the various investigations by means of a questionnaire. The Genie II HIV diagnostic kit was used to confirm HIV positive status. HIV positive subjects were grouped into two: those receiving anti-retroviral therapy were referred to as the ARV group and those not receiving antiretroviral therapy were designated as non-ARV group. Each group was further sub-divided according to the Centers for Disease Control 1993 classification of HIV disease. HIV negative subjects must have tested no later than two months to the sample collection date and must not lead a high-risk lifestyle. Twenty microliters of whole blood were used for the erythrocytes osmotic fragility assay. One milliliter of whole blood was used to prepare the erythrocyte ghost membrane for the Na+-K+ ATPase activity assay. The two HIV positive groups showed significant increase in percentage haemolysis under osmotic stress at 0.65% saline. The ARV group had an average percentage haemolysis of 2.56 ± 0.81% while the non-ARV group had an average of 3.19 ± 1.11% compared to an average of 0.83 ± 0.36% for the control group (p < 0.05). A pattern observed in the result was an increase in activity with increasing severity of the HIV/AIDS disease. Data from the present study indicate that the osmotic fragility of erythrocytes was significantly potentiated, while Na+-K+ ATPase activity was not significantly altered (p < 0.05) in HIV/AIDS disease.

Key Words: Osmotic fragility, Na+ -K+ ATPase activity, erythrocytes, HIV/AIDS

Afr. J. Clin. Exper. Microbiol. 2004; 5(2): 148 – 154.

BLOOD CHEMISTRY AND PLATELET SEROTONIN UPTAKE AS ALTERNATIVE METHOD OF TRACKING HIV/AIDS

OAT Ebuehi, M Balogun, RA Audu, OE Idigbe

 

Abstract

A cross sectional study was conducted to investigate the blood chemistry and platelet serotonin uptake as alternative method of determining HIV disease stage in HIV/AIDS patients. Whole blood was taken from subjects at the Human Virology of the Nigerian Institute of Medical Research. Subjects were judged suitable for the various investigations by means of a questionnaire. The Genie II HIV diagnostic kit was used to confirm HIV positive status. HIV positive subjects were grouped in to two: those receiving antiretroviral therapy were referred to as the ARV group and those not on antiretroviral therapy were designated as non-ARV group. Each group was further sub-divided according to the Centers for Disease Control 1993 classification of HIV disease. HIV negative subjects must have been tested no later than two months to the sample collection date and must not lead a high-risk lifestyle. Serum was used to assay for blood chemistry activities with Randox analytical reagents. Blood platelets were prepared from one milliliter of whole blood and platelet serotonin uptake rates were determined. The serum glutamic-oxaloacetic transaminase (SGOT) of non-ARV subjects was the only blood chemistry parameter that showed any significant variation from normal (p<0.05). The mean activity of this enzyme was 28.4 ± 5.29 U/L compared to a normal value of 12 U/L. A disease stage-related variation was observed. Platelet serotonin uptake rates of the two HIV positive groups showed no significant difference with the HIV negative control. The data obtained showed that serum glutamic-oxaloacetic transaminase activity is significantly increased in HIV/AIDS patients in a manner that is disease stage related. However, serum glutamic-pyruvic transaminase, bilirubin, triglycerides, amylase, serum creatinine, and alkaline phosphatase showed no significant variation from normal values. Platelet serotonin uptake of HIV subjects was not significantly different from the control.

Key Words: Blood chemistry, platelet serotonin uptake, HIV/AIDS

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BLOOD CHEMISTRY AND PLATELET SEROTONIN UPTAKE AS ALTERNATIVE METHOD OF TRACKING HIVAIDS

Afr. J. Clin. Exper. Microbiol. 2004; 5(2): 155 – 159

HUMAN BITE INJURIES IN THE ERA OF AIDS: A REVIEW

SA Malami, SB Zailani

 

Abstract

The risk of human immunodeficiency virus infection (HIV) transmission following human bite is important to many groups of people. Meanwhile, the pandemic of HIV/AIDS continues unabated, with perhaps more than 3 million new infections last year alone. A review of the literature concerning human bite injuries and HIV was performed to examine current opinion regarding the transmission of HIV via this route. It is concluded that human bite contaminated with infected blood carry a small, but definite, risk of transmitting this important life-threatening disease.

Key Words: HIV, Human bite, Risk

Afr. J. Clin. Exper. Microbiol. 2004; 5(2): 160 – 162.