Non-Attenuation Of Highly Pathogenic Avian Influenza H5N1 By Laboratory Exposure To Ultraviolet Rays

FO Fasina, AN Egbuji, DA Gado, DC Nyam, AK Olawuyi, CA Meseko, AT Oladokun, HG Ularamu, S Ponman, I Nwagbo

 

Abstract

Avian influenza H5N1 represents one of the most researched viruses in laboratories world-wide in recent times with regards to its epidemiology, ecology, biology and geography. The virus has caused 409 human cases and 256 human fatalities to date. Some laboratory activities and other lab related
works predispose certain workers to exposure to this virus. In this work, we assessed the effect of exposure of HPAI infective allantoic fluid to ultraviolet rays for between 15 and 180 minutes. No significant difference was found between the unexposed and exposed viruses. The ability of the virus to haemagglutinate chicken red blood cells, the haemagglutination titre and its pathogenicity in embryonating eggs did not change despite this prolong exposure to UV-light. We call for caution in the handling of HPAI viruses in laboratory inside the microbiological safety cabinet despite sterilization using UV-light.

Characterization Of Biocides Resistant Isolates From Dental Unit Water Line Biofilms By Culture Dependent Approach

I Liaqat, AN Sabri

 

Abstract

The importance of biocides resistant bacterial strains in medicine, industry and the environment has gained significant attention. Microbial contamination of dental unit waterlines is thought to be the result of biofilm formation within the small-bore tubing used for these conduits. Our objectives were to characterize biocides resistant isolates from dental unit water line biofilm (DUWL) using the standard laboratory approaches. Growth curves of isolates established in biocides free and supplemented medium demonstrated less growth in the presence of biocides. O10 | P a g e ptimum pH was 7 whereas; optimum temperature was 37°C. Isolates showed resistance against multiple of heavy metals while fewer antibiotics. Genetic studies were accomplished by performing conjugation and transformation experiments. In two isolates (AWT 21 and PTNPF) transconjugants were observed, while no transformant was recorded in any case. Overall, the findings of this study can be used to profile the metabolic effects of new biocides or biocide combinations upon biocides
resistant biofilm isolates from clinical environment.

Effect Of Instructions About The Method Of Urine Collection And Storage On The Isolation Rate Of Urinary Bacteria In Children

SI Adeleke, G Ihesiulor

 

Abstract

A study of 65 children (29males and 36females) and aged between four weeks and 15years with significant bacteriuria was undertaking over a six month period to determine the effects of instruction received about the methods of urine collection and storage on the prevalence of urinary tract infection. The commonest clinical presentation was fever (64.6%). Only 22(35.4%) of the patients had specific symptoms suggestive of urinary tract infections. The instructions about urine collection were given to 48(73.8%) care givers. This instruction was given by the attending doctors (84%). Despite the explanation, 15(23.1%) of the patients collected the urine samples wrongly and 44(67.7%) stored the samples for longer than one hour. Significant bacteriuria was more prevalent in 74.2% of patients who submitted their urine samples more than one hour after collection. Communication skill is important and should be emphasized in the trainings of health workers in procedure on the patients.