THE EFFECT OF SCHISTOSOMA MANSONI CERCARIA INFECTION AND TREATMENT WITH NIRIDAZOLE ON TESTICULAR HISTOLOGY OF THE MICE

SYLVNUS JOSIAH DAFUR, PATRICIA MANKO LAR

 

Abstract

The testicular histology of mice infected with Schistosoma mansoni (S.mansoni) cercaria and treated with Niridazole was examined. The results reveal that infection of mice with Schistosoma mansoni cercariae resulted in distortion of the testicular cyto-architecture including disruption of spermatogenesis as shown by the absence of spermatozoa in the lumen of the sominiferous tubule and distruction of the inter-tubular connective tissue of the infected mice. These changes were reversed to normalcy following two-course treatment of the infected mice with Niridazole after five weeks.

(Af. J. of Clinical and Experimental Microbiology: 2003 4(1): 2-5)

Investigation of the efficacy of two rapid assessment techniques (Optimal 1 and SD-Bioline) for the diagnosis of malaria in rural areas of Nigeria

P.U. Agomo, V.N. Asianya, S.K. Akindele, C.O. Agomo, M.O. Akinyele, T.A. Adewole, U.T. Igbasi, R. C Anyanwu, K.N Egbuna

 

Abstract

We had previously studied the efficacy of three new techniques-Para Sight F, (PSF), Immunochromatographic Test (ICT) and Quantitative Buffy Coat (QBC) – as possible replacements for the time-consuming microscopy in the diagnosis of malaria. Two more rapid assessment techniques (the Optimal 1 and SD-BIOLINE) were recently introduced into Nigeria and claimed to exhibit high sensitivity and specificity. Optimal 1 was particularly claimed to distinguish between P falciparum, P. malariae, P ovale and P. vivax. We have in this work evaluated the efficacy of both the Optimal 1 and SD-Bioline in 240 patients from Ibafo and Magboro Communities in Obafemi-Owode LGA of Ogun State, Nigeria. Results showed that with regard to the detection of P. falciparum, Optimal 1 gave a sensitivity, specificity, positive and negative predictive values of 63.95%, 92.20%, 82.1% and 82.1% respectively, while the SD-Bioline gave 54.84%, 42.9%, 68.0% and 68.0% respectively. In retrospect, the sensitivities shown by 3 other techniques (ICT, PSF and QBC) investigated by us were 88.63, 89.95 and 87.6% respectively. Their specificities on the other hand were 94.60, 91.17, 94.70% respectively. The main advantage of the rapid Optimal 1 technique is that it was able to detect P. malariae which microscopy also detected in three patients. The SD-BIOLINE gave the worst comparative result and could not be recommended for use in Nigeria. This work in conclusion has shown that Optimal 1 could be useful in the rapid diagnosis of the various species of Plasmodium in Nigeria provided the patients could afford the test.

(Af. J. of Clinical and Experimental Microbiology: 2003 4(1): 6-13)