Tag: faeces

^*1Hezil, Dj., ²Zaidi, S., ¹Benseghir, H., ¹Zineddine, R., ³Benamrouche, N., and ¹Ghalmi, F.

 ¹Research Laboratory Management of Local Animal Resources, Higher National Veterinary School, El Alia, Oued Smar, 1615, Algiers, Algeria

²Higher National Veterinary School, El Alia, Oued Smar, 1615, Algiers, Algeria

³Laboratory of Enterobacteria and other related bacteria, Institute Pasteur of Algeria

*Correspondence to: d.hezil@etude.ensv.dz

Abstract:

Background: In cattle, many serotypes of Salmonella enterica are responsible for a wide variety of clinical manifestations, which can cause considerable economic loss. Some serotypes can cause cows to abort sporadically, such as the Dublin serotype. This study was carried out on different cattle farms in the Algiers region to determine the prevalence of Salmonella Dublin using bacteriological and immunological methods.

Methodology: The prevalence of Salmonella was determined by bacteriological analysis in accordance with the reference method AFNOR NF U 47-100 on faecal samples collected from 184 cattle belonging to 19 different farms, and serotyping for S. Dublin. Immunological analysis by enzyme-linked immunosorbent assay (ELISA) for S. Dublin was carried out on milk samples collected from 91 cattle. A survey of case (n=5) and control (n=14) farms for comparative analysis was performed to demonstrate a link between abortion in cows and prevalence of S. Dublin with both bacteriological and immunological methods. Sensitivity, specificity, Cohen Kappa coefficient, McNemar test odds ratios, and confidence intervals were calculated using Winepiscope 2.0 and StatA 9.1 software, and p<0.05 was considered as statistically significant.         Continue reading “Salmonella Dublin associated with abortion in dairy cattle in Algiers and comparison of different diagnostic methods”