Ap-PCR typing of carbapenem sensitive Pseudomonas aeruginosa iIsolated from clinical samples

H D Binnet, G Uraz



In this study the antibiotic susceptibility of 51 P. aeruginosa strains isolated from clinical samples were detected by the disc diffusion test. The susceptibility of P. aeruginosa strains were found as respectively 55% amicacin, 43% aztreonam, 75% netilmycin, 68% sefepim, 73% ceftazidim, 76% ciproflaxacin, 37% gentamicin, 84% meropenem, 76% piperasillin/tazobactam, 47% tobramycin and 84% imipenem. These results show that carbapenems are the most effective antibiotics for P. aeruginosa strains and the efficacy of meropenem and imipenem are high for P. aeruginosa strains Molecular typing profiles of 43 P. aeruginosa strains which are sensitive to meropenem and imipenem antibiotics with AP 1 primary were determined in AP-PCR. As a result of AP-PCR molecular typing study of this 43 P. aeruginosa isolate, no correlation was found out between antibiotic sensitivities and molecular types. This situation once again reveals that reasonable antibiotic usage in absolutely

African Journal of Clinical and Experimental Microbiology Vol. 9 (2) 2008 pp. 64-68

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In vitro Phenotypic Antibiotic Susceptibility Profiles Of Food Indicator Bacteria Isolated From Home-Made Oral Rehydration Solutions In Nigeria

AAO Ogunshe, IL Amusan, AO Oyediran



One thousand and ten bacterial isolates from ORS constituents characterised as Bacillus cereus var. mycoides, Bacillus subtilis, Citrobacter sp., Clostridium perfringes, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Morganella morganii, Proteus mirabilis, P. vulgaris, Pseudomonas aeruginosa, Salmonella enterica serovar Typhi, Salmonella enterica serovar Typhimurium, Shigella dysentariae, Staphylococcus aureus and Vibrio cholerae were screened for their in vitro antibiotic susceptibility profiles using the agar discs and agar well-diffusion methods. The Gram-negative bacteria from granulated sugar samples had 7.69% phenotypic resistance profiles while the Gram-negative bacteria from table salt samples had between 13.3% and 20.0% resistance profiles. The resistance profiles of Gram-positive bacteria from granulated sugar samples was between 8.0% and 19.0% while the Gram-positive bacteria from table salt samples had between 11.0% and 27.9 % resistance profiles towards the test antibiotic (discs). The bacterial isolates from granulated sugar exhibited resistance of between 36.4% in ampicillin + cloxacillin and 64.9% in metronidazole. while the bacterial isolates from table salt gave an overall resistance of 41.0% – 64.7% towards the twenty-eight test oral paediatric antibiotic suspensions All the bacterial isolates from the table salt and granulated sugar samples displayed multiple resistance to the test paediatric antibiotics, except Ps. aeruginosa SA12, Shigella dysenteriae SA16C, SA16D, E. aerogenes SA18A, SA18AE and E. coli SA22A which recorded no (0.0%) resistance to all the test paediatric antibiotics.

Keywords: antibiotics, In vitro, ORS, paediatric, resistance, susceptibility

African Journal of Clinical and Experimental Microbiology Vol. 9 (2) 2008 pp. 69-77

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Rubella IgG antibody in women of child-bearing age in Oyo state

OA Adesina, JA Adeniji, MO Adeoti



230 females of childbearing age from four different towns in Oyo State were screened for rubella specific IgG antibody using a sandwich ELISA test kit. Rubella IgG was detected in 215 (93.5%) of the females. 96% of the non-pregnant, 87.5% of the primigravida and 76% of the multigravida screened positive to the antibody. Ogbomoso, Ibadan, Oyo and Iseyin respectively had 96.1%, 94.2%, 90.5% and 88.2% of their samples positive for the rubella IgG antibody in them. It is imperative for the government to ensure that rubella vaccine is made available and routine so as to avert the risk of congenital rubella syndrome.

African Journal of Clinical and Experimental Microbiology Vol. 9 (2) 2008 pp. 78-81