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Identification and detection of antibiotic susceptibility of the most common anerobes causing infection in surgical hospital, Faculty of Medicine Zagazig University, Egypt

NE El Badawy, RM El shabrawy, RA Ghonaim, Z Allam

 

Abstract

Objectives: Anaerobic infections are considered to be the most difficult organisms to be identified in the microbiology laboratory. It requires strict conditions, proper sampling , long time and laboratory skills. In addition most of them are mixed infections having both aerobic and anaerobic organisms. Choice of the proper antibiotic for treating these anaerobes is live saving for the patient.

Methods: Identification of anaerobic organisms using MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) as a recent tool for identification together with API 20A (as a reference method). Antibiotic susceptibility test was done for the anaerobic isolates using Agar Dilution Method. With the the most commonly used antibiotic in our hospital which are Amoxacillin/Clavulonic acid, clindamycin, metronidazole and Imipenem.

Results: Anaerobic infections constitutes 21.7% of total 249 specimen from different surgical departments. Bacteroids spp. (41%) were the most prevalent anaerobic organisms followed by peptostreptococcus (26.9%). MALDI TOF MS system and API achieved 100% agreement for identification of Porphoryomonas spp. and Fusobacterium, while near results were obtained for other isolates. Bacteroid spp. shows the highest rate of resistance to clindamycin (69%). Excellent results were obtained for Imipenem and metronidazole. Most of resistance to Amoxacillin/Clavulonic acid is related to Bacteroid spp. and Fusobacterium spp.

Conclusion: MALDI TOF MS System is a useful tool for identification of. Anerobes are showing higher rates of resistance to commonly used antibiotics thus detection of resistant strains is vital for proper selection of antibiotics.

Keywords: Anaerobes, MALDI TOF System, API 20, Agar Dilution Method, Zagazig

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Identification and detection of antibiotic susceptibility of the most common anerobes causing infection in surgical hospital, Faculty of Medicine Zagazig

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Evaluation of microbial quality of selected blister-packed paracetamol tablets and paracetamol syrups marketed in Nigeria

MO Osungunna, M Mba, O Adebajo

 

Abstract

Ten brands of blister-packed paracetamol tablet and twenty brands of paracetamol syrup marketed in Nigeria were evaluated for their microbial quality. While no microbial contaminant was isolated from all blistered-packed paracetamol tablets, ten of syrups were contaminated with organisms such as Escherichia coli, Klebsiellaspp, Pseudomonas aeruginosa and Staphylococcus aureus at 14.3, 21.4, 21.4 and 42.9% occurrence respectively. Penicilliumspp was isolated from two brands. Antibiotic susceptibility profile revealed all bacterial isolates to be multidrug resistant with Escherichia coli resistant to all antibiotics tested, while Staphylococcus aureus isolates were sensitive to Oxacillin, Cefuroxime and vancomycin. Pseudomonas aeruginosa isolates were sensitive to ofloxacin and gentamycin while Klebsiella isolates were sensitive to ofloxacin and nitrofurantoin. The study concluded that compliance with the provisions of good manufacturing practice as well as good quality control play role in determining the microbial bioburden of pharmaceutical products while isolation of multi-drug resistant organisms calls for establishment and adherence to antibiotics use policy in Nigeria.

Keywords: Blister-pack, multidrug resistance, good manufacturing practice, quality control, bioburden

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Evaluation of microbial quality of selected blister-packed paracetamol tablets and paracetamol syrups marketed in Nigeria

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Rabies in Nigeria: A review of literature

DT Ojo, VU Nwadike, KI Onyedibe, IE Kalu, KC Ojide

 

Abstract

Rabies, also known as hydrophobia is an acute, viral disease of all warm blooded animals including man. It is caused by the rabies virus (RABV), a bullet–shaped, enveloped RNA virus, 45-100 nm in diameter & 100-430 nm in length with projections and helical nucleocapsid, one of the better known encephalitis viruses of the family Rhabdoviridae and genus Lyssavirus type 1

It is a major public-health problem in most parts of the developing world. The domestic dog (Canis familiaris) plays a principal role (accounting for over 99%) as a reservoir and transmitter of the disease to humans. Developing countries account for almost all the reported human deaths (99.9%) and most cases of human post-exposure treatments. Rabies is an important public health problem especially in the developing countries and this articles aims to draw attention to this neglected disease.

Keywords: rhabdoviridae, rabies

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Rabies in Nigeria A review of literature

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Laboratory information management in a central Nigerian hospital: non-collected or undelivered reports as quality indicator

KC Iregbu, N Abdullahi

 

Abstract

Background: Reporting laboratory reports to the requesting physician is one vital component of the clinical laboratory testing process. Poor management of information generated in the laboratory, such as non collection/non-delivery of test reports, can adversely affect patient care and safety.

Aim: To determine the proportion and financial impact of some laboratory test reports not collected or delivered to the requesting physician.

Methods: A review of laboratory records of requests and collected reports of malaria parasite, urine microscopy, culture and sensitivity, and blood culture from June 2014 to December 2014 was carried out, and data analyzed.

Results: A total of 5321 laboratory requests comprising 4506 malaria parasites (MP), 414 urine microscopy, culture and sensitivity (urine m/c/s), and 410 blood culture were made, processed and reports generated. Of these, 1040 (19.6%) were not collected or delivered to the requesting physician. Urine m/c/s with 37.9% (157/414) accounted for the highest test-specific non-collected reports, closely followed by blood culture with 37.7% (151/401) and MP with 16% (732/4506). ICU with 54.6% (18/33) and A&E with 21% (149/710) accounted for the highest department-specific non-collected or undelivered reports. The cost of all non-collected or delivered reports was N1, 442,560 or 29.3% of the cost of the total requests during the study period.

Conclusion: The proportion of non-collected or undelivered test reports as seen in this study is huge, and indicates a poor laboratory information management system. There is therefore, need to institute and implement appropriate laboratory quality management system to improve patient care and reduce wastage of resources.

Keywords: Information management, Laboratory Report, Central Nigeria

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Laboratory information management in a central Nigerian hospital non-collected or undelivered reports as quality indicator