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Ribonucleic acid extraction: A mini-review of standard methods

Afolabi, Oluwadamilare I.

Research and Development, ARL Bio Limited, Lagos, Nigeria Correspondence to: dare.afolabi@outlook.com; +234 (816) 976-9839

Abstract:

Different techniques have been proposed for RNA extraction, many of which have found extensive use in biological research. The introduction of these methods has greatly improved molecular diagnostics, drug discovery, and numerous other research and clinical endeavors. In this review, the working principles of the most commonly used RNA extraction methods for research and clinical applications are discussed. Current automation efforts and the quest for more efficient and cost-effective methods are highlighted.

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Falciparum malaria is associated with risk markers of type 2 diabetes mellitus in individuals with or without   COVID-19 exposure

[1]Adatsi, R., 2Pappoe, F., 3Bockarie, A. S., 4Derkyi-Kwarteng, L., 5Nsiah, P., 1Weyori, E. W.,

2Dankwa, K., 2Aniakwaa-Bonsu, E., 6Setorglo, J., and *6Acquah, S

1Public Health Reference Laboratory, Tamale Teaching Hospital, Tamale, Ghana

2Department of Microbiology and Immunology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Ghana

3Department of Internal Medicine and Therapeutics, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Ghana

4Department of Pathology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Ghana

5Department of Chemical Pathology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Ghana

6Department of Medical Biochemistry, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Ghana

*Correspondence to: sacquah@ucc.edu.gh

 

Abstract:

Background: Scientific information on the impact of malaria on the risk of developing type 2 diabetes mellitus (T2DM) after recovery from the coronavirus disease 2019 (COVID-19) is limited in the Ghanaian context. The purpose of this study was to examine the association between selected risk markers of T2DM in falciparum malaria patients post-COVID-19 or not at a tertiary hospital in Ghana. Continue reading “Falciparum malaria is associated with risk markers of type 2 diabetes mellitus in individuals with or without   COVID-19 exposure”

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Discordant rate between empirical antibiotics administered and antimicrobial susceptibility in infections caused by Pseudomonas aeruginosa in a tertiary hospital in Nigeria

*1Igunma, J. A., and 1,2Lofor, P. V. O.

1Department of Medical Microbiology, University of Benin Teaching Hospital, Benin City, Edo State, Nigeria

2Department of Medical Microbiology, University of Benin/University of Benin Teaching Hospital,

Benin City, Edo State, Nigeria

*Correspondence to: docigunma@gmail.com; +2348032690163

Abstract:  

Background Early initiation of appropriate antibiotics is key to the effective management of severe bacterial infections.  The initiation of targeted antibiotic therapy is possible only when the causative organism is isolated.  As a result, antibiotics are usually administered on an empirical basis guided by the clinical presentation, local antibiotic guidelines and other relevant histories. Generally, empirical antibiotics differ for both community- and hospital-acquired infections (HAIs), as a result of which common HAI pathogens such as Pseudomonas aeruginosa should be deliberately targeted, because most routine antibiotics are ineffective against them.     Continue reading “Discordant rate between empirical antibiotics administered and antimicrobial susceptibility in infections caused by Pseudomonas aeruginosa in a tertiary hospital in Nigeria”