16S rRNA – afrjcem.org

^{1, 2}*Makolo, D., 2Suleiman, A. B.,²Olonitola, O. S., ³Bello, M., and 4Ahmadu, I.

¹Department of Sciences, School of Preliminary Studies, Kogi State Polytechnic, Lokoja, Nigeria

²Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Nigeria

³Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria

⁴National Tuberculosis Reference Laboratory, Zaria, Nigeria *Correspondence to: makolodaniel@gmail.com

Abstract:

Background: Mastitis caused by Staphylococcus aureus was initially considered the major problem in dairy herds, but over the last few decades, the incidence of coliform mastitis has increased among the pastoral herds in Nigeria due to poor environmental and milking hygiene. Hence, this study was aimed at genotypic identification of coliform bacteria isolated from cases of bovine mastitis among pastoral herds in parts of Kaduna State, Nigeria.

Methods: A cross-sectional survey of 30 herds of cows across 7 Local Government Areas of Kaduna State, Nigeria, was conducted. One hundred and forty seven cows were proportionately selected by purposive sampling technique. The milk samples were aseptically collected and bacteriologically screened for coliform bacteria following standard bacteriological techniques. Nine out of 12 coliforms identified phenotypically were selected for PCR amplification and sequencing of their 16S rRNA gene. The Basic Local Alignment Search Tool (BLAST) analysis of the sequences obtained was done on the National Centre for Biotechnology Information (NCBI) data base, and isolates confirmed based on similarity to 16S rDNA sequences in the Gen Bank Continue reading “Genotypic identification of coliforms isolated from cases of subclinical mastitis among pastoral herds in parts of Kaduna State, Nigeria”

T.A. Bamidele, B.A. Adeniyi, S.I. Smith

Abstract

Background: The antagonistic abilities of lactic acid bacteria (LAB) against clinical isolates of Candida albicans are not quite widely reported and such are even scarce in Nigeria. This study therefore investigated inhibitory potentials of LAB isolated from locally grown cabbage, cucumber and lettuce against four (4) clinical isolates of C. albicans.

Methods: The cell free supernatants (CFS) generated from LAB culture filtrate was evaluated for anti-candida activity using agar well diffusion method, and the CFS-LAB pH was measured and neutralized using standard methods. The proteinaceous inhibitory metabolites were assayed for using sodium dodecylsulphate polyacrilamide gel electrophoresis (SDS-PAGE) technique. The LAB strains used were previously isolated and identified by 16S rRNA partial sequencing and their data submitted to GenBank for accessioning.

Results: The CFS of six (6) LAB strains showed varying degrees of anti-candida activity. Pediococcus pentosaceus BTA 51 from cucumber showed the widest inhibition zone of 14 mm while at neutral pH, it was 12 mm diameter. Weissella confusa BTA 20, BTA 40 isolated from cabbage and lettuce produced 10 mm and 12 mm zones of inhibition at acidic and neutral pH respectively. Lactobacillus plantarum BTA 07 from lettuce showed inhibition zone of 12 mm while L. fermentum BTA 47 and BTA 62 from cucumber showed zones of 14 mm each in acidic pH only. The SDS-PAGE did not detect any proteinaceous substances.

Conclusion: In conclusion, LAB isolated from cabbage, cucumber and lettuce produced organic acids, non proteinaceous metabolites at neutral pH, exhibiting invitro inhibitory abilities against clinical isolates of C. albicans.

Keywords: In vitro, Lactic acid bacteria, 16S rRNA, antifungal, SDS-PAGE, salad vegetables

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In vitro, acidic, non-proteinaceous antifungal activities of lactic acid bacteria isolated from salad vegetables against human pathogenic Candida albicans