1Orjih, C. I., 2Ajayi, A., 1,3Alao, F. O., 1Adeleye, A. I., and *2,4Smith, S. I.
1Department of Microbiology, University of Lagos, Akoka, Nigeria
2Molecular Biology and Biotechnology Department, Nigerian Institute of Medical Research, Lagos, Nigeria
3Department of Biological Sciences, Bells University of Technology, Ota, Ogun State, Nigeria
4Mountain Top University, Lagos-Ibadan Expressway, Ogun State, Nigeria
*Correspondence to: stellaismith@yahoo.com
Abstract:
Background: Biofilm formation by pathogens is of great clinical importance as it mediates persistence and resistance to antibiotics, hence posing difficulty in treatment and management of diseases. The aim of this study was to evaluate the biofilm forming potential of Staphylococcus aureus isolated from urine samples of females with urinary tract infection and to detect the presence of clumping factor (clfA) and intracellular adhesion (icaA) encoding genes.
Methodology: A total of 50 S. aureus were obtained from urine samples of women in five hospitals in Lagos State, Nigeria. Isolates were confirmed by standard biochemical and novobiocin susceptibility tests. The isolates were screened for biofilm formation using three methods; Congo-red agar (CRA), tube, and tissue culture plate (TCP) methods. Detection of clfA and icaA genes was done by PCR. Continue reading “Characterization of biofilm formation in clinical urinary isolates of Staphylococcus aureus from five hospitals in Lagos State, Nigeria”