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Serological study of leptospirosis in cats from Algeria

*[1]Zaidi, S., [2]Amara Korba, A., [3]Bessas, A., 2Bouzenad A., 2Hamnoune, N. K.,   [4],[5]Hezil, Dj., and 6Bitam, I.

1Higher National Veterinary School, El Alia, Oued Smar, 1615, Algiers, Algeria

2Leptospira unit, Pasteur Institute of Algeria, Rue 1 of Doctor Laveran, Hamma Anassers Algiers, Algeria

3Department of Biology, Faculty of Sciences, University of Algiers 1 (Benyoucef Benkhedda University), Algiers, Algeria

4Research Laboratory Management of Local Animal Resources, Higher National Veterinary School of Algeria, ENSV, Algeria

5 Department of Biology, Faculty of Sciences, M’Hamed Bougara University, Boumerdes, Algeria

6Higher School of Food Sciences and Agri-Food Industries, Algiers, Algeria *Correspondence to: zaidi.ensv@gmail.com; s.zaidi@ensv.dz

 

Abstract:

Background: By the nature of their environment and behavior, stray cats are at risk of exposure to leptospirosis. Leptospirosis is an emerging zoonotic disease with worldwide distribution. The prevalence of leptospirosis in the feline species in Algeria is unknown. The main objectives of this study are to determine the seroprevalence and identify the most common Leptospira serovars in stray cats in the Algiers region. Continue reading “Serological study of leptospirosis in cats from Algeria”

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Salmonella Dublin associated with abortion in dairy cattle in Algiers and comparison of different diagnostic methods

*1Hezil, Dj., 2Zaidi, S., 1Benseghir, H., 1Zineddine, R., 3Benamrouche, N., and 1Ghalmi, F.

 1Research Laboratory Management of Local Animal Resources, Higher National Veterinary School, El Alia, Oued Smar, 1615, Algiers, Algeria

2Higher National Veterinary School, El Alia, Oued Smar, 1615, Algiers, Algeria

3Laboratory of Enterobacteria and other related bacteria, Institute Pasteur of Algeria

*Correspondence to: d.hezil@etude.ensv.dz

Abstract:

Background: In cattle, many serotypes of Salmonella enterica are responsible for a wide variety of clinical manifestations, which can cause considerable economic loss. Some serotypes can cause cows to abort sporadically, such as the Dublin serotype. This study was carried out on different cattle farms in the Algiers region to determine the prevalence of Salmonella Dublin using bacteriological and immunological methods.

Methodology: The prevalence of Salmonella was determined by bacteriological analysis in accordance with the reference method AFNOR NF U 47-100 on faecal samples collected from 184 cattle belonging to 19 different farms, and serotyping for S. Dublin. Immunological analysis by enzyme-linked immunosorbent assay (ELISA) for S. Dublin was carried out on milk samples collected from 91 cattle. A survey of case (n=5) and control (n=14) farms for comparative analysis was performed to demonstrate a link between abortion in cows and prevalence of S. Dublin with both bacteriological and immunological methods. Sensitivity, specificity, Cohen Kappa coefficient, McNemar test odds ratios, and confidence intervals were calculated using Winepiscope 2.0 and StatA 9.1 software, and p<0.05 was considered as statistically significant.         Continue reading “Salmonella Dublin associated with abortion in dairy cattle in Algiers and comparison of different diagnostic methods”