*1,2Thonda, O. A., 2Oluduro, A. O., 3Adewole, O. O., and 4Obiajunwa, P. O.
*1Department of Biological Sciences, Microbiology Unit, Kings University, Odeomu, Nigeria
2Department of Microbiology, Faculty of Science, OAU, Ile-Ife, Nigeria
3Department of Medicine, College of Health Sciences, OAU, Ile-Ife, Nigeria
4Department of Paediatrics and Child Heath, Faculty of Clinical Sciences, OAU, Ile-Ife, Nigeria
Correspondence to: thondakemi22@gmail.com
Abstract:
Background: AmpC or class C or group 1 beta lactamases are class C cephalosporinases that hydrolyse a wide variety of beta-lactam antibiotics including alpha methoxy beta-lactams (cefoxitin), narrow and broad spectrum cephalosporins. This study was conducted to characterize plasmid-mediated AmpC producing enteric Gram- negative bacteria from patients with lower respiratory tract infections in Obafemi Awolowo University Teaching Hospital Complex (OAUTHC) Ile Ife, Osun State, Nigeria
Methodology: A total of 149 patients with clinical features of lower respiratory tract infections (LRTI) were selected by simple random sampling for the study. All Gram-negative isolates recovered from standard microbiological cultures of respiratory specimens of these patients were tested against cefoxitin, third generation cephalosporins (3GCs), and other antibiotics using the disc diffusion AST method, and also screened for production of AmpC beta-lactamases phenotypically by the CLSI method. Plasmid DNA extraction was carried out on twenty-nine cefoxitin-resistant selected isolates using the Kado and Lin method, while genotypic detection of plasmid-mediated AmpC gene was carried out by the polymerase chain reaction (PCR) assay. Continue reading “Phenotypic and genotypic characterization of plasmid-mediated AmpC beta-lactamases in enteric Gram-negative bacteria from patients with lower respiratory tract infections in a tertiary hospital, southwest Nigeria”