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Etiologic agents of lower respiratory tract infections among patients attending tuberculosis clinic in Benin City, Nigeria

E.E. Ibadin, H.O. Ogefere, T.O. Ehondor

 

Abstract

The emergence of HIV has reawakened the tuberculosis (TB) scourge and infected patients are prone to opportunistic infections, this study was carried out to determine the HIV status and etiologic agents of Lower respiratory tract infections (LRTI) among tuberculosis suspected patients in Benin City, Nigeria. The study was cross sectional. A total of 276 patients attending tuberculosis clinic were recruited. Questionnaires were filled for each patient. Blood and sputum specimens were collected in plain and sterile containers respectively and transported immediately to the laboratory. Blood samples were screened for the presence of HIV antibodies. The sputum specimens were then cultured following standard microbiological procedure, and thereafter processed using the GeneXpert MTB/RIF assay. Emergent bacterial colonies were identified and susceptibility testing was carried out following standard microbiological techniques. A total of 118 (42.8%) non-mycobacterial bacterial agents were recovered from the patients. Patients were more likely to be culture positive for LRTI in comparison with TB infection (p < 0.0001). There was no association between TB and HIV status (OR = 0.6161, 95%CI = 0.226, 1.648, p = 0.4474). HIV positive patients were more likely to be culture positive for bacterial agents and Klebsiella pneumoniae was more likely to be recovered (p = 0.0338). The fluoroquinolones, gentamicin and ceftriaxone-sulbactam were the most active antibacterial agents against bacterial isolates. The prevalence of LRTI in this study was 52.2%. The study draws attention on the need for physicians to request for bacteriological culture (non-mycobacterial) alongside the TB diagnostic algorithm in suspected TB cases.

Keywords: Tuberculosis, HIV, opportunistic infections, patients, bacteria

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Etiologic agents of lower respiratory tract infections among patients attending tuberculosis clinic in Benin City, Nigeria

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The bacteria profiles of wounds in diabetic patients hospitalized in northern Kwazulu-Natal, South Africa

W. Mthembu, D Penduka, R Mosa, B Shoba, A Zobolo, A Opoku

 

Abstract

Diabetic wound infections still remain a health concern such that correct identification of bacteria is essential in monitoring the spread of the infections as well as in the administration of the correct treatment. This study therefore focuses on isolating and identifying bacteria present in diabetic wounds of hospitalized patients in northern KwaZulu-Natal and assessing their distribution.The wound specimen were collected and swabbed onto selective and differential media. The bacteria identities were presumptively ascertained through biochemical characterization (Gram-stain, catalase test, oxidase test and API) and then confirmed through 16S rDNA sequencing.A total of 42 isolates were recovered from 83% of the patients sampled from the three participating hospitals (X, Y, and Z). Gram-negative bacilli from Enterobacteriaceaewere predominant followed by Staphylococci spp and Enterococcus faecaliswith 43% polymicrobial cases from hospital Z and 29% from hospital X. Distribution of some opportunistic pathogens and nosocomially-acquired pathogens were also observed across the patients with five bacterial identities distributed among hospital X and Z. The adverse effects associated with the recovered bacteria in diabetic wounds pose a serious health concern and preventive measure should be taken.

Keywords: Diabetes mellitus, wounds, bacteria, infection

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The bacteria profiles of wounds in diabetic patients hospitalized in northern Kwazulu-Natal, South Africa

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Nosocomial Urinary Infections at the Urogoly Unit of the National University Hospital (Yalgado Ouedraogo), Ouagadougou: Feb.-Sept. 2012

I Sanou, A Kabore, E Tapsoba, I Bicaba, A Ba, B Zango

 

Abstract

Objective: The aim of this study was to identify the risk factors and the microorganisms susceptibilities of nosocomial urinary infections at the urology unit of the national university hospital of Ouagadougou in Burkina Faso.
Method: From February to September 2012, two bacteriological analyzes have been performed for any of the 75 inpatients in the urology unit of the national university hospital of Ouagadougou in Burkina Faso.
Results: During the study period, 43 cases of nosocomial urinary infection were identified (57.3%) and we found no statistically significant associated risk factors with age groups, sex, arterial blood pressure, kidney illness and urinary obstructive pathologies.
The most frequently isolated bacteria were Escherichia coli (30.9%),  Klebsiella spp (26.9%) and Staphylococcus spp (15.4%). The yeasts strains were very sensitive to antifungal but the bacteria susceptibility rate to antibiotics was very variable. Thus, the cocci were rather sensitive to  association clavulanic acid + amoxicilline and ceftriaxone and enough
sensitive to gentamicine ; the bacilli were enough sensitive to gentamicin and very sensitive to imipenem.
Conclusion: From the antibiogram results, we recommend gentamicin in combination with penicillin or metronidazole as the first antibiotics to be used in the treatment of nosocomial urinary tract infections.

Keywords: urinary infection, nosocomial infection, bacteria, antibiotics

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Nosocomial Urinary Infections at the Urogoly Unit of the National University Hospital Yalgado Ouedraogo Ouagadougou Feb Sept 2012

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Microbial status of smoked fish, scombia scombia sold in Owerri, Imo state, Nigeria

JN Dike-Ndudim, RC Egbuobi, EN Onyeneke, HI Uduji, MA Nwagbaraocha, IA Ogamaka, HM Okorie, LN Egbuobi, AU Opara

 

Abstract

As one of the common sources of protein available to man, fish is highly consumed due to its lower cholesterol content and price. So it forms a rich protein source for both poor and rich. As a part of checkmating the public health risks associated with this general dependence of the population on fish, the microbiological assessment of smoked fish, Scombia, scombia sold in Owerri was embarked on with the aim of ascertaining the microbial quality, the presence and prevalence of microorganisms of public health importance. A total of one hundred and eight (108) samples were collected from the smoking Factory, Open Market and Hawkers. These were analyzed microbiologically for viable heterotrophic bacteria and fungi count on Nutrient and Potato dextrose agar respectively, using pour plate method and coliform count in MacConkey broth by multiple tube method (MPN). The mean value results from the analysis revealed high microbial contamination in all the samples. The resultant data were analyzed statistically using randomized block design of Analysis of Variance (ANOVA) at 95% level of confidence and the difference were separated using the least significance difference (LSD). The mean results of viable  heterotrophic bacteria and fungi count showed no significance difference for the collection sites; but the coliform mean results for the three sites showed marked variation at 95% level (P>0.05). Identified bacteria, include: Staphylococcus aureus, E. coli, Bacillus sp., Klebsiella sp., whereas fungi arePenicillium sp., Aspergilusl sp., Fusarium violaceum, Biospora sp., Candida sp, Botryodioplodia sp., Alternania sp. This high level of microbial contamination can be traceable to handlers, and environment to which this fish is exposed during smoking and selling exercises, and considering the danger it portends to human health, public health and  food safety authorities should intensify their monitoring efforts towards controlling such contamination.

Key words: Bacteria, Yeast, Mould, Smoked fish, Contamination.

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Microbial status of smoked fish, scombia scombia sold in Owerri, Imo state, Nigeria