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Bacteraemia at the tertiary care University Hospital Yalgado Ouedraogo: Bacterial species and their antibiotic resistance profiles

*1,2Kafando, H., 3,4Diallo, I., 5Sanou, G., 6Lompo, P., 1Ouédraogo, G. S., 4,7Sanou, I., 4,8Koeta, F., and 2,9Ouédraogo, A. S.

1Laboratory of Bacteriology-Virology, University Hospital Yalgado Ouedraogo, 03 BP 7022, Ouagadougou, Burkina Faso

2National Reference Laboratory for Antimicrobial Resistance Control (NRL-AMR), Bobo Dioulasso, Burkina Faso

3Department of Infectious Diseases, University Hospital Yalgado Ouedraogo, Ouagadougou, Burkina Faso

4Training and Research Unit in Health Science, University Joseph Ki-Zerbo, Ouagadougou, Burkina Faso

5Departement of Nephrology-Haemodialysis, University Hospital Yalgado Ouedraogo, Ouagadougou,
Burkina Faso

6Institut de Recherche en Sciences de la Sante, Clinical Research Unit of Nanoro (IRSS-URCN), Nanoro, Burkina Faso

7Department of Laboratory, University Hospital of Tengandogo, Ouagadougou, Burkina Faso

8Department of Paediatrics, University Hospital Yalgado Ouedraogo, Ouagadougou, Burkina Faso

9National Institute of Health Sciences, University Nazi Boni, Bobo Dioulasso, Burkina Faso

*Correspondence to: hervekafando7@gmail.com; +22676146695

Abstract:
Background: Bloodstream infections are serious health problem because of the significant morbidity and mortality they cause. The number of deaths increases in the presence of multidrug resistant bacteria. The aim of this study was to determine the bacteriological and resistance profiles of bacteria isolated from blood cultures. Continue reading “Bacteraemia at the tertiary care University Hospital Yalgado Ouedraogo: Bacterial species and their antibiotic resistance profiles”

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Widal antibody titre test versus blood culture; which is a better diagnostic for typhoid fever?   

*1Popoola, O. D., 1Thomas, B. T., 2Folorunso, J. B., 1Balogun-Abiola, H. T., 1Adekola, H. A., 1Okulaja, Q. O., and 1Coker, M. O.

1Department of Microbiology, Olabisi Onabanjo University, Ago Iwoye, Ogun State, Nigeria

2Department of Medical Laboratory, Directorate of Health Services, Olabisi Onabanjo University, Ago Iwoye, Ogun State, Nigeria

*Correspondence to: dorcasatmoment@gmail.com; omolara.popoola@oouagoiwoye.edu.ng; +234-8075773022

ORCID: //orcid.org/0000-0001-6816-5627

 

Abstract:

Background: The importance of accurate diagnosis of infectious diseases is central and crucial to the effectiveness of treatment and prevention of the associated long-term complications of such infections. The objective of this study was therefore to determine the accuracy of the Widal antibody titre test in the diagnosis of typhoid fever relative to the gold standard blood culture technique. Continue reading “Widal antibody titre test versus blood culture; which is a better diagnostic for typhoid fever?   “

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Knowledge, attitude and practice of blood culture: A cross sectional study among medical doctors in a Nigerian Tertiary Hospital

CK Ojide, AI Onwuezobe, EE Asuquo, CS Obiagwu

 

Abstract

Background: Blood culture is one of the most important investigations done in clinical microbiology laboratories. Not only has it been long recognized as the ggold standardh for diagnosis of Blood Stream Infections (BSIs), very important decisions regarding septicaemic patientsf management are based on it. Being a user-dependent diagnostic test, quality of results often depends on the performer.
Aim: To study the knowledge, attitude and practice of blood culture among doctors in a Nigerian tertiary hospital.
Materials and Methods: A pre-tested self-administered semi- structured questionnaire developed by the research team was used to access the biodata, knowledge, attitude and practice of blood culture among doctors in our institution.
Results: Forty-eight (54.5%) out of the 88 doctors studied had good knowledge regarding blood culture, 34 (38.6%) moderate knowledge and 6 (6.8%) poor knowledge. Majority of the senior registrars (75.0%),  registrars (64.3%) and house officers (65.9%) studied had good  knowledge while majority of the consultants (75.0%) had moderate knowledge. Doctors from paediatrics (62.5%) and internal medicine (60.0%) departments had higher proportions with good knowledge  compared to those from surgery (57.9%) and obstetrics and gynaecology (45.0%) (p = 0.240). Majority of the doctors with <10 years experience as doctors (57.0%) had good knowledge compared to 33.3% recorded among those .10 years. Attitude and practice was generally positive.
Conclusion: Through this study areas of unsatisfactory knowledge, attitude and practice of blood culture were identified.
This will help in designing an educational intervention programme for the purpose of addressing identified problems areas in blood culture.

KEYWORDS: Blood culture, Knowledge, Attitude, Practice, Doctors

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Knowledge, attitude and practice of blood culture A cross sectional study among medical doctors in a Nigerian Tertiary Hospital

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Multidrug resistant Salmonellae isolated from blood culture samples of suspected typoid patients in Warri, Nigeria

DA Ehwarieme

 

Abstract

This study investigates the prevalence of R-plasmids in Salmonella sp. isolated from blood samples of suspected typhoid patients in Warri, Nigeria. A total of 136 blood samples were collected between May and December,2009 and screened for the presence of Salmonellae using standard blood culture techniques of which 20(14.7%) was positive for the pathogen. The multidrug resistant (MDR) isolates obtained (n=16; 80.0%), exhibiting the Ampicillin, Chloramphenicol, Cotrimoxazole and Tetracyclin (ACCoT) resistance profile, were subjected to plasmid curing. All (100%) of these MDR isolates bore their resistance markers on plasmids, as they lost their resistance sequel to the curing experiment. The low prevalence (14.7%) of the pathogen in the blood samples indicate that a good number of the suspected typhoid cases may not be incidences of the disease afterall. Furthermore, the high prevalence of MDR and plasmid-mediated MDR (80.0% and 100% respectively) isolates, suggest that treatment failures may be rampant if precise susceptibility test is not conducted prior to prescription.

Key words: Multidrug resistant, blood culture, typhoid fever.

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Multidrug resistant Salmonellae isolated from blood culture samples of suspected typoid patients in Warri Nigeria