*1Bouagnon, J. J. R., 2Bolou, G. E. K., 3Guédé, K. B., 4Sanga, D., 4Koffi, L. R., 4N’Guessan, C. D. R., 5Konan, Y., 5Adjogoua, E. V., 4N’Guessan, J. D.,
4Djaman, A. J., and 1,3Dosso, M.
1Biological Resources Center/Biobank, Institut Pasteur, Côte d’Ivoire
2National Floristic Center, Felix Houphouët-Boigny University, Côte d’Ivoire
3Department of Bacteriology-Virology, Institut Pasteur, Côte d’Ivoire
4Laboratory of Biology and Health, UFR Biosciences, Felix Houphouët-Boigny University, Côte d’Ivoire
5Department of epidemic viruses, Institut Pasteur, Côte d’Ivoire
*Correspondence to: ritabouagnon@pasteur.ci
Abstract:
Background: The present work is part of the exploration of new antiviral molecules to combat antimicrobial resistance. In purpose, this study determined the phytochemical analysis, cytotoxicity and antiviral activity of extracts from three Ivorian medicinal plants; Gliricidia sepium, Ocimum gratissimum and Xylopia aethiopica against poliovirus 1, a non-enveloped RNA virus.
Methodology: Aqueous extract of the three plants, which were identified at the herbarium of National Floristic Center Abidjan, was done using a previously described method. The precipitation or staining technique was used to highlight the chemical groups in the three extracts while the polyphenol content of each extract was assessed by the colorimetric method. Cytotoxicity and antiviral activity tests were performed in 96-well plates. Cytotoxicity of each extract on L20B (a genetically engineered mouse cell line) was determined by observation of the cell line carpet. Antiviral activity of three extracts against poliovirus type I was determined after 72 hours using an assay that measures inhibition of the cytopathic effect on cell culture. Continue reading “Phytochemical study and evaluation of the antiviral activity of aqueous extracts of three medicinal plants; Xylopia aethiopica, Gliricidia sepium and Ocimum gratissimum used in Cote d’Ivoire”