Cox, D., and *Robberts, F. J. L.
Division of Medical Microbiology, University of Cape Town, 1 Anzio Road, Observatory, 7925, South Africa *Correspondence to: lrobberts@gmail.com; +27 645256360; //orcid.org/0000-0003-4397-4852
Abstract:
Background: Diarrhoea is a major cause of morbidity in Cape Town, South Africa, and mortality is attributed to a failure to recognize the severity of the condition. Cryptosporidium and Giardia are increasingly recognized as important causes of diarrhoea in Africa however, suboptimal diagnostic techniques may lead to underappreciation of their significance. Our objectives are to compare the diagnostic yield of direct immunofluorescent antigen (DFA) microscopy on concentrated stool samples for Cryptosporidium and Giardia, with the current approach of wet mount microscopy for Giardia and auramine fluorescent stain for Cryptosporidium on unconcentrated stool. Continue reading “Improved Cryptosporidium case findings using immunofluorescent microscopy on concentrated stool”