*1,2Kara Terki, I., 1Hassaine, H., 3Kara Terki, A., 4Nadira, B., 5Kara Terki, N., 1Bellifa, S., 1Mhamedi, I., and 1,5lachachi, M.
1Laboratory of Food, Biomedical and Environnemental Microbiology (LAMAABE)
2University of Djilali lyabes, Sidi Bel Abbes, Algeria
3University Abou Bekr Belkaid, Tlemcen, Algeria
4Ecole Supérieure de Management, Tlemcen, Algeria
5University Belhadj Chouaib Ain Temouchent, Algeria
*Correspondence to: ibti.kara@gmail.com
Abstract:
Background: Staphylococcus aureus is one of the species of bacteria most frequently isolated from medical devices. The ability to produce biofilm is an important step in the pathogenesis of these staphylococci infection, and biofilm formation is strongly dependent on environmental conditions as well as antibiotics and disinfectants used in the treatment and prevention of infections.
Methodology: In this study, 28 S. aureus isolated from medical devices at the University Hospital Center of Sidi Bel Abbes in Northwestern Algeria were tested for biofilm formation by culture on Red Congo Agar (RCA). The tube method (TM) and tissue culture plate (TCP) techniques were also used to investigate the effect of penicillin, ethanol and betadine on pre-formed biofilm.
Results: Nineteen S. aureus isolates produced biofilm on the RCA and 7 produced biofilms by the tube method, 2 of which were high producer. In addition, 9 S. aureus isolates produced biofilm on polystyrene micro-plates, and in the presence of penicillin and ethanol, this number increased to 19 and 11 biofilm producing S. aureus isolates respectively. On the other hand, no biofilm was formed in the presence of betadine.
Conclusion: It is important to test for biofilm formation following an imposed external constraint such as disinfectants and antibiotics in order to develop new strategies to combat bacterial biofilms but also to better control their formation.
Keywords : Staphylococcus aureus, biofilm, medical device, disinfectant, antibiotic