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Phenotypic detection of extended-spectrum β-lactamase Enterobacterales isolated from people living with HIV/AIDS in Kinshasa, Democratic Republic of the Congo

1Mbusa Vihembo, G., 1Mbundu Lukukula, C., 1Ngeleka Ngoie J., 2Indeko Lomboto, J., 2Mpoyo Munanga, L., 3Nzingula Phasi, O., 4Bayebila Menanzambi, T., 5Denis, O., 1TakaisiKikuni, N. B., 1Liesse Iyamba, J. M., and *1Mulwahali Wambale, J.

1University Reference Center of Antimicrobial Resistance Surveillance (URC-AMRS), Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo

2BOYAMBI Hospital Centre Laboratory, Barumbu, Kinshasa, Democratic Republic of Congo

3Biochemistry Laboratory, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo

4Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo

5National Reference Center for Antimicrobial Resistance in Gram-negative bacilli, CHU UCL NAMUR-Yvoir, Belgium

*Correspondence to: jose.mulwahali@unikin.ac.cd

Abstract:
Background: People living with HIV/AIDS (PLWHA) are prone to opportunistic bacterial infections caused by multidrug-resistant organisms. The aim of this study was to determine the susceptibility of Enterobacterales isolated from urine and stool samples of PLWA attending the BOYAMBI Hospital Center, Kinshasa, Democratic Republic of the Congo to commonly used antibiotics and to detect extended-spectrum β-lactamases (ESBLs) producers among the isolates. Continue reading “Phenotypic detection of extended-spectrum β-lactamase Enterobacterales isolated from people living with HIV/AIDS in Kinshasa, Democratic Republic of the Congo”

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Faecal carriage of extended spectrum β-lactamase producing Enterobacterales (ESBL-PE) in children under five years of age at a tertiary hospital in southwest Nigeria

[1]Abayomi, S. A., 2Oladibu, O. T., 3Lawani, O. A., 4Owolabi, K. I., 2Alabi, A. O.,        and *2Onigbinde, M. O.

1Cancer Research UK, Swansea, Wales, United Kingdom

2Department of Paediatrics and Child Health, College of Health Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria

3Department of Medical Microbiology and Parasitology, University of Ilorin Teaching Hospital, Ilorin, Nigeria

42102 Cole Street, Dudley, West Midlands, United Kingdom                                         

*Correspondence to: moonigbinde@lautech.edu.ng

Abstract:  

Background:  The main reservoir of Enterobacterales is the human gut, which has been reported as a source of hospital acquired infection. Enterobacterales carrying the extended spectrum β-lactamase (ESBL) genes have emerged over the years as significant multidrug resistant (MDR) pathogens, that have hindered effective therapy of infections caused by them, and limited treatment to a small number of drugs such as carbapenems, leading to selection pressure and emergent resistance to carbapenems. The objective of this study was to determine the faecal carriage of ESBL-producing Enterobacterales (ESPL-PE) among children under 5 years of age at the Ladoke Akintola University of Technology Teaching Hospital, Ogbomoso, Nigeria.    Continue reading “Faecal carriage of extended spectrum β-lactamase producing Enterobacterales (ESBL-PE) in children under five years of age at a tertiary hospital in southwest Nigeria”

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Emergence of nosocomial-acquired extensively drug-resistant  and pandrug-resistant Enterobacterales in a teaching hospital in Kuwait

[1]Chadha, A., 1,2Jamal, W., and *3Rotimi, V. O.

Departments of Microbiology, 1Mubarak Al Kabeer Hospital, 2Faculty of Medicine, Kuwait University, Health Sciences Center, Jabriya, Kuwait                      3Department of Microbiology and Parasitology, Faculty of Basic Clinical Sciences, Lagos State University College of Medicine, Ikeja, Nigeria *Correspondence to: bunmivr@yahoo.com

Abstract:

 Background: The emergence and high ascendancy of infections caused by extensively-drug-resistant (XDR) and pandrug-resistant (PDR) Enterobacterales isolates is a serious clinical and public health challenge. Isolation of PDR Gram-negative bacteria (GNB) in clinical setting is very rare and rarer is the infection caused by XDR GNB. Apart from restricted therapeutic options, these infections are associated with increased mortality and morbidity. Urgent studies to re-evaluate existing therapeutic options and research into new antibiotic molecules are desperately needed. The objectives of this study are to report the emergence of rarely encountered multidrug-resistant (MDR), difficult-to-threat, CRE infections in our hospital and investigate their molecular epidemiology.

Methodology: This was a retrospective observational analysis of six patients with severe infections caused by XDR and PDR Enterobacterales isolates at Mubarak AL Kabeer Teaching Hospital, Jabriya, Kuwait, over a period of one and half years. The mechanisms of resistance in these isolates were then prospectively investigated by molecular characterization and genomic studies. Continue reading “Emergence of nosocomial-acquired extensively drug-resistant  and pandrug-resistant Enterobacterales in a teaching hospital in Kuwait”