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Phenotypic detection of extended-spectrum β-lactamase Enterobacterales isolated from people living with HIV/AIDS in Kinshasa, Democratic Republic of the Congo

1Mbusa Vihembo, G., 1Mbundu Lukukula, C., 1Ngeleka Ngoie J., 2Indeko Lomboto, J., 2Mpoyo Munanga, L., 3Nzingula Phasi, O., 4Bayebila Menanzambi, T., 5Denis, O., 1TakaisiKikuni, N. B., 1Liesse Iyamba, J. M., and *1Mulwahali Wambale, J.

1University Reference Center of Antimicrobial Resistance Surveillance (URC-AMRS), Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo

2BOYAMBI Hospital Centre Laboratory, Barumbu, Kinshasa, Democratic Republic of Congo

3Biochemistry Laboratory, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo

4Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo

5National Reference Center for Antimicrobial Resistance in Gram-negative bacilli, CHU UCL NAMUR-Yvoir, Belgium

*Correspondence to: jose.mulwahali@unikin.ac.cd

Abstract:
Background: People living with HIV/AIDS (PLWHA) are prone to opportunistic bacterial infections caused by multidrug-resistant organisms. The aim of this study was to determine the susceptibility of Enterobacterales isolated from urine and stool samples of PLWA attending the BOYAMBI Hospital Center, Kinshasa, Democratic Republic of the Congo to commonly used antibiotics and to detect extended-spectrum β-lactamases (ESBLs) producers among the isolates. Continue reading “Phenotypic detection of extended-spectrum β-lactamase Enterobacterales isolated from people living with HIV/AIDS in Kinshasa, Democratic Republic of the Congo”

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Prevalence and risk factors for extended-spectrum β-lactamaseproducing Gram-negative bacterial infections in hospitalized patients at a tertiary care hospital, southwest Nigeria

*1Adeyemo, A. T., 2Adeyemo, A. T., 3Odetoyin, B. W., and 2,3Onipede, A. O.
1Department of Medical Microbiology and Parasitology, Uniosun Teaching Hospital, Osogbo, Nigeria
2Department of Medical Microbiology and Parasitology, Obafemi Awolowo University Teaching Hospitals
Complex, Ile-Ife, Nigeria
3Department of Medical Microbiology and Parasitology, Obafemi Awolowo University, Ile-Ife, Nigeria
*Correspondence to: adeyemoat@gmail.com; +2347031287078

Abstract:
Background: Clinical infections caused by extended-spectrum β-lactamase (ESBL)-producing bacteria constitute great burden to healthcare delivery with these resistant pathogens contributing largely to the magnitude and spread of antimicrobial resistance globally. Hence, knowledge of the risk factors for acquisition of infection caused by ESBL-producing bacteria is crucial to instituting prompt and appropriate treatment as well as prevention and control measures. This study investigated the risk factors associated with the prevalence of ESBL-producing Gram-negative bacteria (GNB) infections among hospitalized patients in Uniosun Teaching Hospital (UTH), Osogbo, Nigeria.

Methodology: A total of 359 hospitalized patients with clinical infections from whose clinical samples we isolated non-duplicate GNB were consecutively recruited. GNB were isolated following aerobic cultures of
appropriate clinical samples and MicrobactTMGNB 24E kit was used for species identification. All isolates were screened for ESBL production by the combination disc method. Relevant clinical and demographic information
was obtained using a designed data collection form, and multivariate logistic regression analysis was used to identify associated risk factors. Continue reading “Prevalence and risk factors for extended-spectrum β-lactamaseproducing Gram-negative bacterial infections in hospitalized patients at a tertiary care hospital, southwest Nigeria”

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Microbiological and kinetic detection of gram negative bacilli producing extended-spectrum- β-lactamases (esbl) in emergencies and reanimation units of university hospital center, Yalgadoouedraogo, Burkina Faso

KJ Zongo, A Metuor Dabire, B Kaborel, I Sanou, L Sangare, J Simpore, B Zeba

 

Abstract

Background: Epidemiology of extended-Spectrum- β-lactamases has become worldwide, and our aim was to establish the prevalence of isolates producer in university hospital center Yalgado OUEDRAOGO particularly in reanimation and emergencies units.

Material and methods: Prospective study was drive during July 2009 to march 2012 in order to collect strains resisting to third generation of cephalosporin during diagnosis analysis of biological specimens. Susceptibility of bacteria to antimicrobial agents was evaluated by disc diffusion method. Production of extended-spectrum β-lactamases has been investigated by double disc diffusion and kinetic methods.

Results: 259 isolates which resisted at least to one of third generation of cephalosporins were collected. Among them 188 (72, 58 %) were positive to synergy test by a double disc diffusion method. The MICs of ceftriaxone determined by E-test were under than 50kg/ml, 100kg/ml et 256kg/ml for respect 81,57°/° ; 55,26°/° et 39,74°/° of isolates. Hydrolyze of β-lactam ring by bacterial extract followed at spectrophotometer showed speeds running at 0 to 0,090UAb.mn-1 for both isolates. Extract of 171 bacterial strains positives to synergy test had hydrolyzed at least one of oxy-iminocephalosporins and were identified as producing extended- spectrum β-lactamases. Spices reported by this study were 99 Escherichia coli (57,89%) ; 28 Klebsisella pneumonia (16,37%) ; 15 Enterobactersp (8,77%) ; 19 Pseudomonas aeruginosa (11,11%) ; 4 Citrobactersp (2,33%) 2 Acinetobactersp (1,16%), 3 Proteus mirabilis (1,75%) and 1 Salmonella typhi (0,05%).

Conclusion: This study showed that bacterial resistances by extended- spectrum β-lactamases are a reality in University Hospital center YalgadoOuedraogo. It calls about antibiotics prescription and hospital hygiene in order to reduce emergence and propagation of new resisting bacterial.

Keywords: microbial and kinetic analysis, Gram negative bacilli, extended-Spectrum- β-lactamase, emergencies, reanimation

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Microbiological and kinetic detection of gram negative bacilli producing extended-spectrum- β-lactamases (esbl) in emergencies and reanimation units of university hospital center, Yalgadoouedraogo, Burkina Faso