Salmonella Dublin associated with abortion in dairy cattle in Algiers and comparison of different diagnostic methods

*1Hezil, Dj., 2Zaidi, S., 1Benseghir, H., 1Zineddine, R., 3Benamrouche, N., and 1Ghalmi, F.

 1Research Laboratory Management of Local Animal Resources, Higher National Veterinary School, El Alia, Oued Smar, 1615, Algiers, Algeria

2Higher National Veterinary School, El Alia, Oued Smar, 1615, Algiers, Algeria

3Laboratory of Enterobacteria and other related bacteria, Institute Pasteur of Algeria

*Correspondence to: d.hezil@etude.ensv.dz

Abstract:

Background: In cattle, many serotypes of Salmonella enterica are responsible for a wide variety of clinical manifestations, which can cause considerable economic loss. Some serotypes can cause cows to abort sporadically, such as the Dublin serotype. This study was carried out on different cattle farms in the Algiers region to determine the prevalence of Salmonella Dublin using bacteriological and immunological methods.

Methodology: The prevalence of Salmonella was determined by bacteriological analysis in accordance with the reference method AFNOR NF U 47-100 on faecal samples collected from 184 cattle belonging to 19 different farms, and serotyping for S. Dublin. Immunological analysis by enzyme-linked immunosorbent assay (ELISA) for S. Dublin was carried out on milk samples collected from 91 cattle. A survey of case (n=5) and control (n=14) farms for comparative analysis was performed to demonstrate a link between abortion in cows and prevalence of S. Dublin with both bacteriological and immunological methods. Sensitivity, specificity, Cohen Kappa coefficient, McNemar test odds ratios, and confidence intervals were calculated using Winepiscope 2.0 and StatA 9.1 software, and p<0.05 was considered as statistically significant.         Continue reading “Salmonella Dublin associated with abortion in dairy cattle in Algiers and comparison of different diagnostic methods”

Isolation of Enterovirus from Feacal Samples of Patients with Diabetes Mellitus in Maiduguri, Nigeria

BS Oderinde, MM Baba, MU Nwodo, J Ladan, PE Ghamba, MT Akinola, AM Kadai

 

Abstract

In this study, 150 patients were recruited out of which 63(42%) were male while 87(58%) were female subjects. Patients with type 1 diabetes were 2(1.3%), those with type 2 were 142(94.7%) while those with GDM were 4(4%). Only one sample from type 2 was positive by virus isolation and identified to be Echovirus 1 and 21 by microneutralization tests as described in WHO polio laboratory manual, 2004. It has been demonstrated that enterovirus infections were significantly more common in recently diagnosed diabetic patients, compared to control subjects. The question if enterovirus could cause beta cell damage and diabetes mellitus has become more and more relevant when recent studies have provided new evidence supporting this scenario especially in type 1 diabetes. This is an important issue since it opens the possibility to develop new, preventive and therapeutic strategies to fight the disease. The purpose of this study is to investigate if enterovirus can be isolated from the stool samples of diabetic patients as a study.

Key words: Isolation, enteroviruses, faeces, diabetes mellitus, patient

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Isolation of Enterovirus from Feacal Samples of Patients with Diabetes Mellitus in Maiduguri, Nigeria