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Klebsiella pneumoniae producing extended spectrum β-lactamase in Regional Military University Hospital of Oran, Algeria: antibiotic resistance, biofilm formation, and detection of blaCTX-M and blaTEM genes

*1Benbrahim, C., 1Barka, M. S., 2Benmahdi, L., 3Zatout, A., and 1Khadir, A.

1Laboratory of Applied Microbiology in Food, Biomedical and Environment (LAMAABE), Department of Biology, Faculty of Nature and Life, Earth and Universal Sciences, Abou Bekr Belkaid University, 13000 Tlemcen, Algeria

2Laboratory of Microbiology, Regional Military University Hospital, Oran, Algeria

3Laboratory of Microbiology and Plant Biology, Department of Biological Sciences, Faculty of Natural Sciences and Life, University of Abdlhamid Ibn Badis, Mostaganem, Algeria

*Correspondence to: chahla.benbrahim@univ-tlemcen.dz

Abstract:
Background: Klebsiella pneumoniae is a bacterial pathogen commonly associated with severe nosocomial and community acquired infections especially through the acquisition of extended spectrum β-lactamases (ESβL) and biofilm formation capacity. The objectives of this study are to determine the prevalence of K. pneumoniae ESβL (KP-ESβL)-producing isolates in the Regional Military University Hospital of Oran (HMRUO) Algeria, characterize their antibiotic resistance profile, genetically detect blaTEM and blaCTX-M genes, and evaluate their biofilm formation capacity. Continue reading “Klebsiella pneumoniae producing extended spectrum β-lactamase in Regional Military University Hospital of Oran, Algeria: antibiotic resistance, biofilm formation, and detection of blaCTX-M and blaTEM genes”

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High prevalence of extended-spectrum β-lactamase (blaCTX-M-15) and New Delhi metallo-β-lactamase-1 (NDM-1) genes among high-level carbapenem resistance Klebsiella pneumonia: an alarm for our health system

S. Yaghoubi, Z Baseri, A. Rasti, M. Gharani, Y. Erfani

 

Abstract

Background: The extended-spectrum β-lactamase (ESBL) carbapenems-resistant Klebsiella isolates are considered one of the most significant challenging in the treatment of patients in hospitals. The aim of this study was to determine the prevalence of important carbapenem resistance genes ESBL subtypes and between K. pneumoniae from patients at hospital in Tehran, Iran.
Methods: Fifty-four isolates of K. pneumoniae were isolated from Shariatee Hospital in Tehran from February 2013 to July 2016. Antibiotic testing was done by using the standard disk diffusion method and E-test MIC. The confirmation of carbapenemase activity was performed using an MHT and a new method called the carbapenem inactivation method test (CIM). Finally, a polymerase chain reaction (PCR) and sequencing of related genes was performed.
Results: Our PCR data demonstrate that blaCTX-M group’s 40 (81.4%) genes were the most prevalent in our hospital followed by group genes blaCTX-M-3 (18.51%) and blaCTX-M-2 (20.38%). The distribution of the CTX-M group revealed that blaCTX-M-15 23 (42.6%) was the dominant subtype. The coexistence of multiple genes included blaTEM, CTX-M and blaSHV, and CTX-M The presence of blaNDM1, blaOXA-48, and blaKPC were identified in the carbapenem-resistant isolates, 22 (40.7%), 10 (18.5%), and 7 (12.9%) respectively.
Conclusion: Our research showed that a CIM test for the first time in Iran is possible and has a high facility for the fast identification of carbapenem-resistant Klebsiella (CRK). We are encountered with the emergence of CTX-M, OXA-48, KPC, and NDM1 harboring CRK strains in our hospitals. Therefore, the treatment of patients infected with these isolates will be an important future concern in our clinical settings.

Running Head: Resistance genes among carbapenem-resistant Klebsiella pneumonia

Keywords: New Delhi metallo-beta-lactamase-1, Klebsiella pneumoniae, Carbapenem, Extended-spectrum ß-lactamase

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High prevalence of extended-spectrum β-lactamase (blaCTX-M-15) and New Delhi metallo-β-lactamase-1 (NDM-1) genes among high-level carbapenem resistance Klebsiella

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First molecular investigation of capsular serotyping and hypervirulent (hvlp) of K. Pneumoniae in university hospital center of yopougon cote d’ivoire

A. M’lan-Britoh, S. Meité, C. Boni, F. Zaba, K. S. Koffi, N. Guessennd, N. S. Kakou, H. Faye- Kette, M. Dosso

 

Abstract

Klebsiella pneumoniae is a well known human pathogen. Although infectious in most nosocomial infections with a high level of resistance, capsular types and circulating hypervirulent strains in our context are not documented. The aims of this study are to identify capsular serotypes and hypervirulent strains circulating at the Yopougon University Hospital in Abidjan. 51 strains of Klebsiella were collected at Chu de Yopougon. The capsular serotypes were determined using PCR and the serotypes K1, K2 and K5 were searched. The hypervirulent strains were also investigated by PCR and by string test. The predominant serotypes were non-K1 / K2 (46/51, 90%). The serotypes found K5 and K2 in (4/51, 7.8%) and (1/51; 1.9%) respectively. The rmpA gene linked to hyperviscosity or hyperviscosity was not found although 25.5% (12/51) were positive for the stretch test. The capsular distribution of strains of Klebsiella pneumoniae seems different from Asian authors. The determination of non-K1non types K2 remains to be elucidated.

Keyvords: Klebsiella pneumoniae, capsular serotype, hypervirulence

Premiere etude d’investigation moleculaire de serotypage capsulaire et de gene d’hypervirence de klebsiella pneumniae au laboratoire du chu de yopougon en cote d’ivoire

Klebsiella pneumoniae est un pathogène nosocomial humain bien connu. Bien qu’incriminé dans la plus part des infections nosocomiales avec un niveau élevé de résistance, les types capsulaires et les souches hypervirulentes circulantes dans notre contexte ne sont pas documentés. L’objectif de cette étude est d’identifier les sérotypes capsulaires et les souches hypervirulentes circulant au CHU de Yopougon Abidjan., 51 souches de Klebsiella ont été collectés au Chu de Yopougon. Les sérotypes capsulaires ont été déterminée à l’aide de la PCR et les sérotypes K1, K2 et K5 ont été recherchés. Les souches hypervirulentes ont été recherchées également par PCR et par le test d’étirement ou string test. Les sérotypes prédominants étaient les non K1/K2 (46/51; 90%). Les sérotypes retrouvés K5 et K2 dans respectivement (4/51; 7,8%) et (1/51 ; 1,9%). Le gène rmpA lié à l’hyperviscosité n’a pas été retrouvé bien que 25,5% (12/51) étaient positives au test d’étirement. La distribution capsulaire des souches de Klebsiella pneumoniae semble différente des auteurs asiatiques. D’ou l’intérêt de travaux plus approfondies afin de déterminer les types capsulaire des souches non K1 non K2.

Mots clefs : Klebsiella pneumoniae, serotype capsulaire , Hypervirulence

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First molecular investigation of capsular serotyping and hypervirulent (hvlp) of K. Pneumoniae in university hospital center of yopougon cote d’ivoire

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Epidemiologic characteristics of Klebsiella pneumoniae isolates in ventilator-associated pneumonia in intensive care units

A.A. El Sharkawy, M.A. Mansour, H.M. Helmy, D.M. Abd El Azeem

 

Abstract

Klebsiella pneumoniae is a common pathogen that causes ventilator associated pneumonia (VAP) in intensive care units (ICUs). Strain typing is a useful tool in tracking the spread of these infections. Primary objective was to study different strains causing VAP in Anesthesia ICUs. Secondary objective was to determine role of health-care workers (HCWs) and ICU environment in the transmission of these strains. Endotracheal aspirates of 60 VAP patients, surveillance samples from the HCWs (18 )and the ICU environment (193)were collected. Antibiogram typing and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) were used for comparison of the isolates from VAP patients and surveillance samples. Antibiogram showed 5 antibiotic susceptibility patterns that were designated A1-A5. ERIC-PCR yielded 1 to 5 amplification bands. All the isolates were typable by ERIC-PCR. Eight ERIC patterns were obtained ERIC(I)-ERIC(VIII). ERIC-PCR typing method gave higher discriminatory index (D) (0.7557) than antibiogram (0.6035). There was sharing of certain ERIC patterns among patient and HCWs or environmental sources. In Conclusion: K.pneumoniae is the most dominant pathogen in anesthesia ICUs. Throats and hands of HCWs are possible sources of pathogen transmission to patients. Surfaces with hand contact of the medical staff are often contaminated and may serve as vectors for cross transmission.

Keywords: Ventilator-associated pneumonia, ICU environment, health-care workers, Klebsiella pneumoniae, antibiogram typing, ERIC-PCR

Epidemiologic characteristics of Klebsiella pneumoniae isolates in ventilator-associated pneumonia in intensive care units