Prevalence of carbapenemase production in Pseudomonas aeruginosa isolates causing clinical infections in Lagos University Teaching Hospital, Nigeria

1Ettu, A. O., *2Oladapo, B. A., and 2Oduyebo, O., O.
1Department of Medical Microbiology and Parasitology, Lagos University Teaching Hospital, Nigeria
2Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Nigeria

*Correspondence to: [email protected]; 08053505952

Abstract:
Background: Pseudomonas aeruginosa has been highly associated with carbapenem resistance in which carbapenemases has been suggested to be a major contributory factor. Hence the objective of this study was to phenotypically detect KPC-type carbapenemase, metallo-β-lactamase and OXA-48 carbapenemase production in clinical isolates of P. aeruginosa in Lagos University Teaching Hospital (LUTH), Nigeria Continue reading “Prevalence of carbapenemase production in Pseudomonas aeruginosa isolates causing clinical infections in Lagos University Teaching Hospital, Nigeria”

Biochemical and immunological characterization of haemolysin produced by Pseudomonas aeruginosa PAO1 isolated from burn wounds

*1Allam, A. A., 2El-shawadfy, A. M., 2Hassanein W. A. E., 2Hamza, E. H. A., 1Morad, E. A., 1El Shafei, M. A. E., and 3El Etriby, D. E.

1Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Egypt

2Botany and Microbiology Department, Faculty of Science, Zagazig University, Egypt

3Infection Control Unit, Specialized Internal Medicine Hospital, Mansoura University, Egypt

*Correspondence to: [email protected]; 0020155777174; 00201227989609

Abstract:

Background: Infection of burn wounds by multidrug-resistant (MDR) Pseudomonas aeruginosa (P. aeruginosa) is a leading cause of morbidity and mortality and remains one of the most challenging concerns for the burns unit. The aim of this study is purify and characterize the haemolysin produced by multidrug resistant P. aeruginosa PAO1 isolated from burn wounds.

Methods: Isolation and identification of P. aeruginosa from burns was done by standard bacteriological methods. P. aeruginosa PAO1 was identified by PCR amplification and sequencing of the 16S rRNA gene. The haemolysin of P. aeruginosa PAO1 was purified by 70% ammonium sulphate precipitation followed by gel filtration on Sephadex G-100, and separation by SDS-Poly Acrylamide Gel Electrophoresis. In vivo toxicity of the purified haemolysin was determined by intraperitoneal injection of Swiss albino mice, and in vitro toxin-antitoxin neutralization test was performed as previously described. Continue reading “Biochemical and immunological characterization of haemolysin produced by Pseudomonas aeruginosa PAO1 isolated from burn wounds”

Otitis externa in a tertiary care hospital in Zagazig, Egypt: isolated pathogens and their antibiotic sensitivity patterns

1*Allam, A. A. E., 2Tantawy, A. E. E., 2Mohamed, K. A. E., 3El ghamrey, N. A. M., 1Morad, E. A., and 1El Shafei, M. A. E.

1Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Egypt 2ENT Department, Faculty of Medicine, Zagazig University, Egypt 3ENT specialist, Egypt Ministry of Health and Population *Correspondence to: [email protected]; +20155777174, +201227989609

Abstract:

Introduction: Recurrent otitis externa is a worldwide problem. This study aims to identify the different aetiological organisms isolated from otitis externa and their sensitivity to different antibiotics. Methods: A total of 27 patients with clinical presentation of otitis externa for a period of three weeks or more were enrolled for the study. Two swab samples collected from each infected ear were cultured for bacterial and fungi, and growth identified using standard microbiological methods including analytical profile index (API) system. Antibiotic susceptibility of isolated bacteria was performed by the disk diffusion technique. Results: Thirty one organisms were isolated from the 27 patients; 12 (38.7%) fungi and 19 (61.3%) bacteria species. Aspergillus spp was the most frequently isolated organism (35.4%) while Pseudomonas aeruginosa was the most frequently isolated bacteria (19.3%), and was most sensitive to amikacin. Four of 11 patients with Aspergillus infection showed clinical resistance to econazole local treatment but had complete clinical response to itraconazole oral treatment.

Conclusion: Otitis externa in Egypt is caused by antibiotic resistant bacteria or fungi, and the most causative organisms are Aspergillus spp and Ps. aeruginosa.

Keywords: Otitis externa, antibiotic resistance, Pseudomonas aeruginosa,

Egypt Received June 25, 2019; Revised September 3, 2019; Accepted September 19, 2019
Copyright 2020 AJCEM Open Access. This article is licensed and distributed under the terms of the Creative Commons Attrition 4.0 International License (//creativecommmons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium, provided credit is given to the original author(s) and the source.

 

Otite externe à l’hôpital de soins tertiaires de Zagazig, en Égypte: agents pathogènes isolés et leur profil de sensibilité aux antibiotiques

1*Allam, A. A. E., 2Tantawy, A. E. E., 2Mohamed, K. A. E., 3El ghamrey, N. A. M., 1Morad, E. A., et 1El Shafei, M. A. E. 1Département de microbiologie médicale et d’immunologie, Faculté de médecine, Université de Zagazig, Égypte 2Département d’ORL, Université de Zagazig, Faculté de médecine, Égypte 3spécialiste ENT, Ministère égyptien de la santé et de la population
*Correspondance à:[email protected]; +20155777174, +201227989609

Abstrait:
Introduction: L’otite externe récurrente est un problème mondial. Cette étude vise à identifier les différents organismes étiologiques isolés d’une otite externe et leur sensibilité à différents antibiotiques. Méthodes: Un total de 27 patients présentant une présentation clinique de l’otite externe sur une période de trois semaines ou plus ont été inclus dans l’étude. Deux échantillons de prélèvement prélevés sur chaque oreille infectée ont été mis en culture pour détecter la présence de bactéries et de champignons, et leur croissance a été identifiée à l’aide de méthodes microbiologiques standard, notamment d’un système d’indice de profil analytique (API).

sensibilité aux antibiotiques de bactéries isolées a été réalisée par la technique de diffusion sur disque. Résultats: Trente et un organismes ont été isolés parmi les 27 patients; 12 espèces de champignons (38,7%) et 19 espèces de bactéries (61,3%). Aspergillus spp était l’organisme le plus fréquemment isolé (35,4%), tandis que Pseudomonas aeruginosa était la bactérie la plus fréquemment isolée (19,3%) et était la plus sensible à l’amikacine. Quatre des 11 patients infectés par Aspergillus ont présenté une résistance clinique au traitement local à l’éconazole, mais ont présenté une réponse clinique complète au traitement oral à l’itraconazole. Conclusion: L’otite externe en Egypte est causée par une bactérie ou un champignon résistant aux antibiotiques. Les organismes les plus responsables sont Aspergillus spp et Ps. aeruginosa.

La Otitis externa in Egypt Afr. J. Clin. Exper. Microbiol. 2020; 21 (1): 60-65

Mots-clés: otite externe, résistance aux antibiotiques, Pseudomonas aeruginosa, Égypte

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Otitis externa in a tertiary care hospital in Zagazig, Egypt: isolated pathogens and their antibiotic sensitivity patterns

Pseudomonas aeruginosa Infections in a Tertiary Hospital in Nigeria

KC Iregbu, SO Eze

 

Abstract

BackgroundPseudomonas aeruginosa is a known opportunistic pathogen frequently causing serious infections. It exhibits innate resistance to a wide range of antibiotics thus causing high rates of morbidity and mortality worldwide.
Objective: This study was done to determine the distribution and the antibiotic susceptibility pattern in clinical isolates of Pseudomonas aeruginosa in NHA.
Method: Laboratory data on 265 Pseudomonas aeruginosa isolates from a total of 30,384 clinical specimens processed over a 3 year period (January 1st 2010 to December 31st 2012) were analyzed.
Results: A total 30,384 samples were submitted for bacteriologic analysis, 265 (1%) yielded Pseudomonas aeruginosa of which 195 (74%) were from in-patient sand 70 (26%) fromout-patients.185 (70%) isolates were from adults while 80 (30%) were from children. 87% of the isolates were susceptible to imipenem, 77% to amikacin, while 34% were resistant to ciprofloxacin and 46% resistant to ceftazidime.
Conclusion: The relatively high proportion of resistance to ciprofloxacin and ceftazidime, and the emerging resistance to amikacin and imipenem are worrisome and calls for rational antibiotic use and institution of effective resistance surveillance and infection control measures.

KeywordsPseudomonas aeruginosa, National Hospital Abuja, Susceptibility

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Pseudomonas aeruginosa Infections in a Tertiary Hospital in Nigeria

Effect Of Essential Leaf Oil Of Ocimum Gratissimum On Clinical Isolates Of Pseudomonas aeruginosa

BA Iwalokun, IG Owuh, SA Ronke

 

Abstract

Ocimum gratissimum leaf oil, which has been reported to possess in vitro and in vivo efficacy against enteric bacteria was tested against forty six clinical isolates of Pseudomonas aeruginosa in Lagos, Nigeria. The effect of the essential oil (EO) on pyocyanin production among these strains was also investigated. Agar well diffusion assay revealed susceptibility in 40 (87%) of the 46 tested strains with inhibition zone diameter (20-36 mm) comparable with the effect of tobramycin. Of the 40 susceptible isolates, 34 strains were quantitatively demonstrated to show susceptibility when further tested with the essential oil in broth and on agar yielding MIC and MBC values of 36 – 54 mg/ml and 42 – 66 mg/ml respectively. The values were higher than the 12 mg/ml (MIC) and 24 mg/ml (MBC) observed in Escherichia coli ATCC 25922. Compared with the control, the essential oil was found to reduce pyocyanin production significantly (p < 0.01) at 15 mg/ml (30.1 – 30.5 vs 259.2 – 276. 7 μg/ml) and 75 mg/ml (2.5 – 3.5 vs. 259.2 – 276.7 μg/ml) in both sensitive and resistant strains, suggesting that Ocimum gratissimum leaf oil may inhibit expression of virulence factors and progression of Pseudomonas infections caused by the tested strains.

Key Words: Ocimum gratissimum leaf oil, Pseudomonas aeruginosa, Nigeria

Afr. J. Clin. Exper. Microbiol. 2004; 5 (3): 265-271

ANTIBIOTIC SUSCEPTIBILITY PATTERN AND MULTIPLE ANTIBIOTIC RESISTANCE INDEX OF PSEUDOMONAS AERUGINOSA URINE ISOLATES FROM A UNIVERSITY TEACHING HOSPITAL

BO Olayinka, OS Olonitola, AT Olayinka, EA Agada

 

Abstract

Urine samples submitted to the Medical Microbiology diagnostic laboratory of the Ahmadu Bello University Teaching Hospital, Zaria, were routinely screened for Pseudomonas aeruginosa over a three-month period with 13/150 (8.67%) of the pathogenic bacteria isolated positively identified. All the isolates were resistant to the cheap, commonly available antibiotics; rifampicin, ampicillin/cloxacillin, erythromycin, chloramphenicol and ampicillin but were uniformly susceptible to ciprofloxacin. The high prevalence of multidrug resistance indicates a serious need for broad-based, local antimicrobial resistance surveillance for continuous tracking of antibiotic resistance trends among all clinically relevant isolates and introduction of effective interventions to reduce multidrug resistance in such pathogens.

Key Words: Pseudomonas aeruginosa, antibiotic susceptibility, multiple antibiotic resistance, urinary tract infection

Afr. J. Clin. Exper. Microbiol. 2004; 5(2): 198 – 202.