*[1]Zouré, A. A., 1Compaoré, T. R., 2Beré, J. A., 1Sagna, T., 1Soubeiga, S. T., 1Dabiré, C., 1Nikiema, A. R., 3Barro, N., and 1Ouedraogo, H. G.
1Department of Biomedical and Public Health, Institute of Health Sciences Research (IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso
2Unité de Formation et de Recherche en Science et Technique (UFR/ST), Université Catholique de l’Afrique de l’Ouest Unité Universitaire à Bobo Dioulasso (UCAO/UUB)
3Laboratoire de Biologie Moléculaire, d’Epidémiologie et de Surveillance des Agents, Transmissibles par les
Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques Alimentaires et Nutritionnelles (CRSBAN),
Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso
*Correspondence to: abdouazaque@gmail.com; +22625363215; ORCiD: //orcid.org/0000–0002–9423–024X
Abstract:
Background: The extraction step of the viral material of the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) influences the quality of reverse transcriptase-polymerase chain reaction (RT-PCR) results in diagnosis of coronavirus disease 2019 (COVID-19). The purpose of this cross-sectional study was to evaluate the diagnostic performance of the automated extraction system “KingFisher Flex Purification System 96 (ThermoFisher)” compared to the manual method with the “QIAamp Viral RNA Mini Kit (Qiagen)”.
Methodology: From October to December 2020, comparative diagnostic evaluation of two methods of SARSCoV-2 RNA extraction methods was conducted on 159 fresh and 120 frozen nasopharyngeal and oropharyngeal specimens collected from travellers and suspected cases or contacts of COVID-19 patients in Burkina Faso. The FastPlexTM Triplex 1-Step COVID 19 Detection Kit (RT-PCR, RNA extraction free) (Precigenome LLC) was used to amplify on the same PCR plate, RNA extracts from manual QIAamp Viral RNA Mini Kit and automated KingFisher Flex Purification System 96 (ThermoFisher) using the QuantStudio5 thermal cycler (Applied Biosystems). Analysis of the diagnostic performance of the SARS-CoV-2 RT-PCR assay following RNA extraction by the two methods was done using an online OpenEpi software. Continue reading “Comparative evaluation of automated KingFisher Flex Purification System 96 (ThermoFisher Scientific) and manual QIAamp Viral RNA Mini Kit (Qiagen) extraction methods for SARS-CoV-2”