The bacteria profiles of wounds in diabetic patients hospitalized in northern Kwazulu-Natal, South Africa

W. Mthembu, D Penduka, R Mosa, B Shoba, A Zobolo, A Opoku

 

Abstract

Diabetic wound infections still remain a health concern such that correct identification of bacteria is essential in monitoring the spread of the infections as well as in the administration of the correct treatment. This study therefore focuses on isolating and identifying bacteria present in diabetic wounds of hospitalized patients in northern KwaZulu-Natal and assessing their distribution.The wound specimen were collected and swabbed onto selective and differential media. The bacteria identities were presumptively ascertained through biochemical characterization (Gram-stain, catalase test, oxidase test and API) and then confirmed through 16S rDNA sequencing.A total of 42 isolates were recovered from 83% of the patients sampled from the three participating hospitals (X, Y, and Z). Gram-negative bacilli from Enterobacteriaceaewere predominant followed by Staphylococci spp and Enterococcus faecaliswith 43% polymicrobial cases from hospital Z and 29% from hospital X. Distribution of some opportunistic pathogens and nosocomially-acquired pathogens were also observed across the patients with five bacterial identities distributed among hospital X and Z. The adverse effects associated with the recovered bacteria in diabetic wounds pose a serious health concern and preventive measure should be taken.

Keywords: Diabetes mellitus, wounds, bacteria, infection

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The bacteria profiles of wounds in diabetic patients hospitalized in northern Kwazulu-Natal, South Africa

In vitro and in vivo antimicrobial activity of partially purified enterocin produced by Enterococcus faecalis and its application in wound healing

O.M. David, M.O. Alese, D.M. Komolafe, I.J. Adejare, O.O. Alese, A.E. Omonisi

 

Abstract

Background: The recent global upsurge in antibiotic resistance among bacteria associated with wounds has contributed to high treatment failures in wound management. Enterocin are produced by enterococci and has been reported to inhibit the growth of many bacteria including those associated with wound infections.

Objectives: In this study, antibacterial and physico-chemical properties of partially purified enterocin (PPE) from Enterococcus faecalis was determined. Also, the possible application of the enterocin in wound management was evaluated.

Materials and Methods: Eight different enterocin were tested and that with highest antibacterial (E3) was partially purified using standard methods. The molecular weight of the PPE was determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis after which the in vitro anti-Staphylococcus aureus potential of the PPE was determined.

Results: Enterocin (E3) was effective against Staphylococcus aureusKlebsiella pneumoniae, Enterobacter cloacaListeria monocytogenes and Proteus vulgaris. The activity of the E3 was very prominent at pH of 4 and 8. The molecular size of the isolated enterocin was 5.5 KDa. The photomicrograph of the skin tissue of the skin treated with partially purified enterocin for day 7showed epidermis covered by atrophic stratified squamous epithelium. A synergistic interaction was noticed when Eusol was used with the enterocin.

Conclusions: From this study, enterocin from E. faecalis has a low molecular weight and inhibited bacteria isolates from wound and also aids physiological healing of wound.The antibacterial potency of this bacteriocin indicates that it is an alternative therapeutic agent that can be employed in wound care and management.

Keywords: Enterocin, Enterococcus faecalis, wounds, bacteriocin, Staphylococcus aureus, skin

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In vitro and in vivo antimicrobial activity of partially purified enterocin produced by Enterococcus faecalis and its application in wound healing