Vol 24, No 3

Emergence of clinical vanA-type vancomycin-resistant Staphylococcus aureus isolates in National Orthopaedic Hospital Dala, Kano, Nigeria

Prevalence and risk factors for hepatitis C virus infection among HIV positive patients at the Lagos University Teaching Hospital, Nigeria

Seroprevalence of transfusion transmissible infections by ELISA in donors testing negative with rapid ICT in Asokoro District Hospital, Abuja, Nigeria

Emergence of New Delhi metallo-β-lactamase-1 (NDM-1) producing Enterobacterales from water sources: an impending public health challenge in Adamawa-north senatorial zone, Nigeria

Isolation of Globicatella sanguinis from a neonate with sepsis using BacT/Alert and VITEK-2 compact system at Federal Teaching Hospital, Katsina, northwest Nigeria: A case report

Antimicrobial properties, safety, and probiotic attributes of lactic acid bacteria isolated from Sauerkraut

*Fadare, O. S., Anyadike, C. H., Momoh, A. O., and Bello, T. K.

Department of Biological Sciences (Microbiology Unit), Faculty of Basic and Applied Sciences, Elizade University, Wuraola Ade Ojo Avenue, P. M. B. 002, Ilara-Mokin, Ondo State, Nigeria

*Correspondence to olalekan.fadare@elizadeuniversity.edu.ng; +2348169001041; ORCiD: 0000-0002-1749-2238

 

Abstract:

 Background: According to the World Health Organization, probiotics have been defined as live microorganisms that when administered in the right amount provide health benefits to the host. This study aims to isolate lactic acid bacteria (LAB) from Sauerkraut and determine their anti-pathogenic potential and suitability as probiotics.

Methodology: Lactic acid bacteria (LAB) were isolated from fermented cabbage obtained from an open market in Akure, Ondo State, Nigeria by inoculating the prepared cabbage suspension on triplet plates of Rogosa agar (Lactobacillus selecting agar) and incubating in an anaerobic jar with gaspaks at 37oC for 72 hours. The LAB isolates were presumptively identified phenotypically by colony morphology, Gram stain reaction, and catalase test. The antibacterial activity of the LAB isolates was then carried out using agar overlay and agar-well diffusion methods. The most efficient LAB isolate was selected based on its strong antibacterial activity, confirmed by 16S rRNA sequencing and further evaluated for probiotic activities including bile salt resistance, survival in low pH, hydrophobicity of the cell surface, auto-aggregation, and co-aggregation. The putative probiotic LAB isolate was also evaluated for its safety using in vitro tests (antibiotic susceptibility testing, haemolysis, and DNase tests) and in vivo assays (sub-acute oral toxicity tests in male albino rats). Continue reading “Antimicrobial properties, safety, and probiotic attributes of lactic acid bacteria isolated from Sauerkraut”