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Urinary tract infections in pregnancy caused by carbapenem-resistant Enterobacteriaceae in University of Calabar Teaching Hospital, Nigeria: An emerging therapeutic threat

1,2Ogban, G. I., *1,2Iwuafor, A. A., 3Idemudo, C. U., 2Ben, S. A., 4Ushie, S. N., and 1,2Emanghe, U. E.

1Department of Medical Microbiology and Parasitology, University of Calabar, Calabar, Nigeria

2Department of Medical Microbiology and Parasitology Main Laboratory, University of Calabar Teaching Hospital, Calabar, Nigeria

3Department of Public Health, University of Calabar, Calabar, Nigeria 4Department of Medical Microbiology and Parasitology, Nnamdi Azikiwe University, Awka, Nigeria *Correspondence to: tonyiwuafor@unical.edu.ng; +2348033441539

Abstract:
Background: Severe infections caused by carbapenem-resistant Enterobacteriaceae (CRE) have mortality rate exceeding 50%. On the strength of this, this study sought to determine the prevalence of urinary tract infection (UTI) in pregnancy caused by CRE and associated risk factors in University of Calabar Teaching Hospital (UCTH), Nigeria, with the aim of making recommendations that can stem the tide of UTI caused by this bacterial strain in the hospital. Continue reading “Urinary tract infections in pregnancy caused by carbapenem-resistant Enterobacteriaceae in University of Calabar Teaching Hospital, Nigeria: An emerging therapeutic threat”

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Antimicrobial resistance profiles of bacteria from Enterobacteriaceae family of laying chicken in Ibadan, southwestern Nigeria

*1Ojja, C. V., 2Amosun, E. A., and 3Ochi, E. B.

1,2Avian Medicine Programme, Pan African University Life and Earth Sciences Institute (Including Health and Agriculture), University of Ibadan, Ibadan, Nigeria

2Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria

3Department of Clinical Studies, School of Veterinary Medicine, University of Juba, Juba, South Sudan *Correspondence to: ojja.christopher@paulesi.org.ng; vukenichris170@gmail.com; +211923419563

Abstract:
Background: Antibiotics are significant for improving the health and productivity of chickens, but overuse and misuse of antibiotics have led to the development of antimicrobial resistance (AMR), which has resulted in ineffective treatment of infectious diseases with associated mortality in chicken and potential spread of AMR pathogens to humans. The objective of the study was to evaluate the AMR profiles of Enterobacteriaceae from faecal samples of laying chicken in Ibadan, southwestern Nigeria. Continue reading “Antimicrobial resistance profiles of bacteria from Enterobacteriaceae family of laying chicken in Ibadan, southwestern Nigeria”

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Antibiotic resistance profiles of uropathogenic bacterial isolates in Haut-Sassandra Region, Côte d’Ivoire from January 2019 to December 2022

 

[1]Gbégbé, D. A., 1,2N’zi, N. P., 3Monthaut, S., 2Guessennd-Kouadio, N., and *1Angaman, D. M.

1Department of Biochemistry-Microbiology, Jean Lorougnon Guédé University, Daloa, P.O. Box 150 Daloa, Côte d’Ivoire

2Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute Côte d’Ivoire, P. O. Box 490 Abidjan 01, Côte d’Ivoire

3Bacteriology-Virology Laboratory, Regional Hospital Center of Daloa, P. O. Box 207 Daloa, Côte d’Ivoire *Correspondence to: angaman@ujlg.edu.ci

 

Abstract:

Background: The escalating issue of bacterial resistance is a profound universal peril. This looming crisis has evolved from a mere forecast to a tangible reality globally. Urinary tract infections (UTIs) significantly influence antibiotic prescriptions in primary care, thus crucially impacting the selective pressure and the emergence of antibiotic-resistant bacteria. A profound comprehension of the microorganisms involved in UTIs and their resistance patterns is crucial, particularly in Daloa city, Côte d’Ivoire. This research aims to review the antibiotic resistance profiles of uropathogens isolated from patients in the Regional Hospital Center (CHR) of Daloa, Côte d’Ivoire from January 2019 to December 2022. Continue reading “Antibiotic resistance profiles of uropathogenic bacterial isolates in Haut-Sassandra Region, Côte d’Ivoire from January 2019 to December 2022”

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High faecal carriage of extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-PE) among hospitalized patients at Sylvanus Olympio Teaching Hospital, Lomé, Togo in 2019

*1,2Godonou, A. M., 2Lack, F., 3,4Gbeasor-Komlanvi, F. A., 2Konlani, L., 2,3Dossim, S., 1Ameyapoh, Y. A., 3,4Ekouevi, K., 2,3Dagnra, A. Y., and 3,5Salou, M.

1High School of Biological and Food Technics, University of Lomé, Togo

2Teaching Hospital Sylvanus Olympio, Lomé, Togo

3Faculty of Health Sciences, University of Lomé, Togo

4Faculty of Health Sciences Public Heath Department, University of Lomé, Togo

5Teaching Hospital Campus, Lomé, Togo *Correspondence to: godonouvirginie@yahoo.fr

Abstract:
Background: Extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-PE) are a global health concern, associated with increased morbidity and mortality. Even in the absence of infections, colonization by these pathogens is still a great threat because of the risk of cross transfer among hospitalized patients. Faecal carriage of ESBL-PE remained poorly documented in Africa. This study aimed to determine faecal carriage rate of ESBL-PE, factors associated with carriage, and antimicrobial susceptibility of the strains among hospitalized patients at Sylvanus Olympio Teaching Hospitals (CHU SO) in Lomé, Togo. Continue reading “High faecal carriage of extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-PE) among hospitalized patients at Sylvanus Olympio Teaching Hospital, Lomé, Togo in 2019”

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Faecal carriage of multi-drug resistant Enterobacteriaceae in hospitalized children at University Teaching Hospital Sylvanus Olympio of Lomé, Togo

1Lack, F., 1Tsogbalé, A., 2Doumegno, J. K., 3Dossim, S., 1Dagnra, A., and *1,4Salou, M.

1Laboratory of Molecular Biology and Immunology, Faculty of Health Sciences, University of Lomé, Lomé, Togo

2Laboratory of Microbiology and Food Quality Control, University of Lomé, Togo

3CHU Kara Medical Biology Laboratory, Kara, Togo

4National AMR Reference Laboratory CHU Campus, Lomé, Togo

*Correspondence to: mounerous@gmail.com

Abstract:
Background: High prevalence of infections and associated antibiotherapy may put children at increased risk for development of multidrug-resistance (MDR), mostly to bacterial infections. The objective of this study therefore was to determine the prevalence of gastrointestinal carriage of MDR Enterobacteriaceae among hospitalized children in the Paediatric department of Sylvanus Olympio University Hospital, Lomé, Togo. Continue reading “Faecal carriage of multi-drug resistant Enterobacteriaceae in hospitalized children at University Teaching Hospital Sylvanus Olympio of Lomé, Togo”

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Antimicrobial resistance patterns and transferable traits in Enterobacteriaceae isolates from poultry in Tlemcen, Algeria

*Barka, M. S., Cherif-Anntar, A., and Benamar, I.

Laboratory of Applied Microbiology in Food, Biomedical and Environment (LAMAABE), Science Department,

Applied Science and Technique Institute, University of Tlemcen, Tlemcen, Algeria

*Correspondence to: mohammedsalih.barka@univ-tlemcen.dz; Tel: 00213556123335; Fax: 0021343277405

Abstract:

Background:  Antibiotics are overused in poultry industry, and this has resulted in the emergence of multidrug resistant (MDR) bacteria. The current study is aimed at determining antimicrobial resistance (AMR) patterns of Enterobacteriaceae isolates from poultry in the west of Algeria.

Methodology: Different chicken samples (kidney, bone and intestine) were collected and processed for culture using standard microbiological methods to isolate Enterobacteriaceae. Isolates were identified biochemically using API 20E, while isolated Escherichia coli was typed for O1, O2 and O78 antigens using slide agglutination with specific antisera. All identified isolates were tested against 26 antibiotic disks using the Kirby Bauer disk diffusion method according to the CLSI standards. The minimum inhibitory concentrations (MICs) of chloramphenicol, tetracycline, nalidixic acid, ofloxacin and ciprofloxacin were determined for selected isolates.  Conjugative plasmid transfer, plasmid incompatibility and colicin tests were used to detect transferable resistance traits in 48 selected E. coli isolates. Continue reading “Antimicrobial resistance patterns and transferable traits in Enterobacteriaceae isolates from poultry in Tlemcen, Algeria”

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Prevalence and factors associated with extended-spectrum β-lactamase producing Enterobacteriaceae bacteraemia in University Hospital of Befelatanana, Madagascar

*1Rakotovao-Ravahatra Z. D., 2Randriatsarafara, F. M., 3Rakotovao, A. L., and 3Rasamindrakotroka, A.

1Laboratory of Joseph Raseta Befelatanana, University Hospital, Antananarivo, Madagascar

2Public Health Department, Faculty of Medicine, University of Antananarivo, Madagascar

3Medical Biology Department, Faculty of Medicine, University of Antananarivo, Madagascar *Correspondence to: ravahatradomoina@yahoo.fr; +261 34 09 301 20

Abstract:
Background: The extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae are a major cause of nosocomial bacteraemia. The objectives of this study are to describe the antibiotic resistance pattern of ESBL producing Enterobacteriaceae responsible for bacteraemia and identify factors associated with these infections in a University Hospital in Madagascar. Continue reading “Prevalence and factors associated with extended-spectrum β-lactamase producing Enterobacteriaceae bacteraemia in University Hospital of Befelatanana, Madagascar”

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Laboratory survey of extended spectrum beta-lactamase producing Enterobacteriaceae from selected tertiary hospitals in south-eastern Nigeria

*1Ugah, U. I., and 2Udeani, T. K.

1Department of Microbiology, Faculty of Science, Alex Ekwueme Federal University, Ndufu-Alike 2Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, University of Nigeria, Enugu Campus *Correspondence to: uchenna.ugah@funai.edu.ng; +2347062154353

Abstract:

Background: Extended spectrum beta-lactamases are produced by Gram-negative bacteria and most strains producing them belong to the family Enterobacteriaceae. The greatest challenge with ESBL-producing Enterobacteriaceae is their propensity to acquire multidrug resistance traits. This study aimed at determining the prevalence of ESBL-producing Enterobacteriaceae among selected tertiary hospitals in south-eastern Nigeria.

Methods: A total of 400 Enterobacteriaceae isolates were obtained from patients attending five selected tertiary hospitals and were identified to species level by Gram staining and conventional biochemical tests. Screening for ESBL production was determined by the Kirby-Bauer disk diffusion method using 30μg disk of ceftriaxone, cefuroxime, cefpodoxime, ceftazidime, and aztreonam while confirmatory test was done using combination disk test based on the 2016 CLSI guidelines.

Results: The prevalence of ESBL production among Enterobacteriaceae isolates from selected hospitals in southeast Nigeria is 61.5% (246 of 400). Among the isolates obtained, the highest prevalence was observed in Klebsiella oxytoca (100%) while the least prevalence was seen in Morganella morganii (50.0%). Escherichia coli and Klebsiella pneumoniae had rates of 61.8% and 62.3% respectively. Among the States of the south-east Nigeria, selected hospital in Ebonyi had a prevalence of 83.5%, Abia 63.6%, Anambra 61.5%, Enugu 51.7% and Imo 36.5%. The prevalence of ESBL-producing Enterobacteriaceae differ significantly between the States (p=0.000).

Conclusion: ESBL-producing Enterobacteriaceae strains have been isolated from different participants, from the selected tertiary hospitals in south-eastern Nigeria. Therefore, we report a high prevalence of ESBL-producing Enterobacteriaceae in south-eastern Nigeria.

Keywords: ESBL, Enterobacteriaceae, resistant strains, southeast Nigeria

Received Feb 12, 2020; Revised March 27, 2020; Accepted March 28, 2020

Copyright 2020 AJCEM Open Access. This article is licensed and distributed under the terms of the Creative Commons Attrition 4.0 International License <a rel=”license” href=”//creativecommons.org/licenses/by/4.0/”, which permits unrestricted use, distribution and reproduction in any medium, provided credit is given to the original author(s) and the source.

Enquête en laboratoire sur les entérobactéries productrices de bêta-lactamases à spectre étendu de certains hôpitaux tertiaires du sud-est du Nigéria

*1Ugah, U. I., et 2Udeani, T. K. Continue reading “Laboratory survey of extended spectrum beta-lactamase producing Enterobacteriaceae from selected tertiary hospitals in south-eastern Nigeria”

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Antimicrobial susceptibility of extended-spectrum beta-lactamase producing Enterobacteriaceae causing urinary tract infections in Ouagadougou, Burkina Faso

D.S. Kpoda, N Guessennd, N.S. Somda, A Ajayi, J.I. Bonkoungou, F Konan, M.B. Ouattara, M Somda, J Simpore, R Ouedraogo, K.M. Drabo, L Sangare, M Dosso, A.S. Traore

 

Abstract

Objective: To determine the frequency of extended-spectrum beta lactamase producing Enterobacteriaceae (ESBL) and other antibioticsresistant bacteria in urinary tract isolates.

Study Design: prospective and experimental study.

Methodology: Place and duration of study :YalgadoOuedraogo University Hospital Center, Charles De Gaulle Pediatric Hospital Center, Saint Camille Hospital and National Public Health Laboratory, Ouagadougou, from November 2014 to October 2015.

AllEnterobacteriaceaestrains isolated from urinary samples of patients were identifiedusing API 20E chemical gallery (BioMerieux, France). All strains were subjected to an array of 14 antibiotics to study their drug susceptibility by using Kirby- Baeurdisk diffusion method. Detection of ESBL was carried out by double disk diffusion technique. Statistical analysis was performed by Microsoft Excel and Anova one-way GrapPad Prism version 5.01. Chi-square (χ2) test was used to determine significance. A p˂ 0.05was considered to be statistically significant.

Results: A total of 324 isolates of Enterobacteriaceae were identified during the study period, including211(65%) E. coli, 75 (23%)Klebsiellaspp., 18 (6%) Enterobacter spp., 11 (3%)Proteus spp., 5 (2%) Citrobacter spp., Serratia spp. 3 (1%).All the clinical isolates were susceptible to imipenem. Resistance to amikacinwas 14% (45/324); gentamicin 54% (175/324); tobramycin 58% (187/324); nalidixic acid 72% (234/324),ciprofloxacin 63% (204/324) and to cotrimoxazole 83% (269/324).The overall rate of the EBSL producing strains was 35% (114/324). Their susceptibility to antibiotics was (imipenem,amikacin, cefoxitin and fosfomycin) 100% (114/114), 93% (106/114), 74% (84/114) and 84% (96/114) respectively. ESBL positivity within individual organism group was highest inEscherichia coli 64% (73/324) followed byKlebsiellaspp. 28% (32/324), Enterobacterspp. 3% (4/324), Proteus spp. and Citrobacterspp. 2% (2/324).

Conclusion: The results showeda high frequency of ESBL producing Enterobacteriaceae, especially Escherichia coli and Klebsiellaspp. The data points to theneed of routine detection and surveillance of ESBL producing bacteria in Burkina Faso.

Keywords: Antimicrobial susceptibility, Enterobacteriaceae, Urine, Burkina Faso

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Antimicrobial susceptibility of extended-spectrum beta-lactamase producing Enterobacteriaceae causing urinary tract infections in Ouagadougou, Burkina Faso

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Occurrence of Extended-Spectrum Beta-Lactamase Producing Enterobacteriaceae Isolates in Communal Water Sources in Ogun State, Nigeria

BT Odumosu, AR Akintimehin

 

Abstract

The role of Enterobacteriaceae in dissemination and reservoir of antibiotic resistance genes in outbreaks of disease and infections are pressing public health concern. This study is aimed at investigating the antibiotic resistance patterns and ESBL production in water-borne Enterobacteriaceae recovered from some selected drinking water sources among 6 towns in
Ogun State (Nigeria). Sixty water samples were collected from boreholes and well waters sources, of these samples 68 Enterobacteriaceaeincluding Enterobacter spp, Escherichia coli, Klebsiella spp, Salmonella spp, Citrobacter freundii, Serratia spp were recovered and identified presumptively using standard microbiological and biochemical methods. The susceptibilities of the isolates to nine antibiotics were carried out by disk diffusion method and determination of ESBL production was by double-disk synergy method. Of the 68 isolated strains tested, 1 (1.47%) was susceptible to all the
antibiotics, 98.5% of the isolates were resistant to . 1 antibiotics and 75% were found to be resistant to . 2 classes of antibiotics. Incidence of water-borne ESBL-producing enteric in this study was 7.14%. The increasing unabated spread of Enterobacteriaceae in public water supply harboring resistance genes portends a high risk for communal outbreaks. This necessitates an urgent precautionary and antibiotics surveillance measures in Nigeria.

Keywords: Enterobacteriaceae, antibiotic resistance, ESBL

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Occurrence of Extended-Spectrum Beta-Lactamase Producing Enterobacteriaceae Isolates in Communal Water Sources in Ogun State, Nigeria