Posted on

Molecular characterization of extended spectrum beta-lactamase among clinical multidrug resistant Escherichia Coli in two hospitals of Niamey, Niger

A. M. Fody, T. S Bagré, A. K. Traoré, A Yacouba, R. Dembelé, L. Boubou, A. Inoussa, R. Sidikou, A. S. Traoré, A. Gassama-Sow, N. Barro

 

Abstract

Objective: The aim of this study was to identify the multiple ESBL genes in Multidrug-resistant (MDR) Escherichia coli isolated in various biological samples in two hospitals of Niamey.
Methodology: A total of 195 multidrug-resistant Escherichia coli were included in the study. These isolates were tested using polymerase chain reaction (PCR) for detection of the presence of bla CTX-M, bla TEM, bla SHV and bla OXA-1 beta-lactamase genes.
Results: A total of 27.7% of Escherichia coli isolates were ESBL producing strains. Globaly, the bla TEM gene was the most prevalent (70.3%) followed by bla CTX-M (43.1%), bla OXA-1 (31.8%) and bla SHV (4.1%) genes. The four genes type of ESBL were founded simultaneously only in stool samples. Furthermore, none bla SHV gene was found in other samples type.
Conclusion: This study showed the presence of various ESBL genes among clinical MDR Escherichia coli. That is why a rational use of antibiotic and appropriate methods of screening ESBL genes in routine laboratories in Niger is needed to control the ESBL genes dissemination.

Keywords: MDR ,Escherichia coli, ESBL, bla genes, PCR, Niamey, Niger.

 

Caracterisation moleculaire des betalactamases a spectre etendu chez les souches de Escherichia coli multi resistantes dans deux hopitaux de Niamey, au Niger

Objectifs: Le but de cette étude était d’identifier les multiples gènes de BLSE chez les souches de Escherichia coli multi résistantes isolées de différents types d’échantillons biologiques dans deux hôpitaux de Niamey.
Méthodologie : Un total de 195 Escherichia coli multi résistants a été inclus dans l’étude. Ces isolats ont été testés par la réaction de polymérase en chaîne (PCR) pour détecter la présence des gènes bla CTX-M, bla TEM, bla SHV et bla OXA-1.
Résultats : Au total, 27,7% des isolats de Escherichia coli multi-résistants étaient des souches productrices de BLSE. Globalement le gène blaTEM (70,3%) était le plus détecté suivi des autres gènes bla CTX-M (43,1%), bla OXA-1 (31,8%) et bla SHV (4,1%). Notons que seul dans les échantillons de selles quatre types de gènes de BLSE ont été trouvés simultanément. Par ailleurs notons qu’aucun gène de type bla SHV n’a été trouvé dans les autres types d’échantillons.
Conclusion : Cette étude avait montré la présence de divers gènes de BLSE chez les souches cliniques de Escherichia coli. C’est pourquoi une utilisation rationnelle des antibiotiques et des méthodes appropriées de dépistage des gènes de BLSE dans les laboratoires sont nécessaires afin de contrôler la diffusion des gènes de BLSE.

Mots clés : Escherichia coli multi résistantes, BLSE, gènes bla, PCR, Niamey, Niger

Download full journal in PDF below

Molecular characterization of extended spectrum beta-lactamase among clinical multidrug resistant Escherichia Coli in two hospitals of Niamey, Niger

Posted on

Occurrence of Extended-Spectrum Beta-Lactamase Producing Enterobacteriaceae Isolates in Communal Water Sources in Ogun State, Nigeria

BT Odumosu, AR Akintimehin

 

Abstract

The role of Enterobacteriaceae in dissemination and reservoir of antibiotic resistance genes in outbreaks of disease and infections are pressing public health concern. This study is aimed at investigating the antibiotic resistance patterns and ESBL production in water-borne Enterobacteriaceae recovered from some selected drinking water sources among 6 towns in
Ogun State (Nigeria). Sixty water samples were collected from boreholes and well waters sources, of these samples 68 Enterobacteriaceaeincluding Enterobacter spp, Escherichia coli, Klebsiella spp, Salmonella spp, Citrobacter freundii, Serratia spp were recovered and identified presumptively using standard microbiological and biochemical methods. The susceptibilities of the isolates to nine antibiotics were carried out by disk diffusion method and determination of ESBL production was by double-disk synergy method. Of the 68 isolated strains tested, 1 (1.47%) was susceptible to all the
antibiotics, 98.5% of the isolates were resistant to . 1 antibiotics and 75% were found to be resistant to . 2 classes of antibiotics. Incidence of water-borne ESBL-producing enteric in this study was 7.14%. The increasing unabated spread of Enterobacteriaceae in public water supply harboring resistance genes portends a high risk for communal outbreaks. This necessitates an urgent precautionary and antibiotics surveillance measures in Nigeria.

Keywords: Enterobacteriaceae, antibiotic resistance, ESBL

Download full journal in PDF below

Occurrence of Extended-Spectrum Beta-Lactamase Producing Enterobacteriaceae Isolates in Communal Water Sources in Ogun State, Nigeria

Posted on

Detection of Amp C Beta Lactamases in Clinical Isolates of Escherichia coli and Klebsiella

CO Akujobi, NN Odu, SI Okorondu

 

Abstract

Detection of AmpC-mediated resistance in Gram negative organisms poses a problem due to misleading results in phenotypic tests. There are no recommended guidelines for detection of this resistance mechanism and there is a need to address this issue as much as the detection of extended spectrum beta lactamases (ESBLs) since both may co-exist and mask each other. Several methods have been used to detect the presence of AmpC β-lactamase production in some isolates but most of these methods are not reliable. There is a need for a reliable method of evaluating the presence of AmpC β-lactamases in clinical isolates. A total of 81 consecutive non repetitive clinical isolates of Escherichia coli(n=40) and Klebsiella spp. (n=41) were screened for AmpC production by disc diffusion method using cefoxitin (30 Zg) disc and confirmed by inhibitor based test using boronic acid as inhibitor. A total of 16 E.coli isolates (40%) and 16 Klebsiella isolates (39.02%) screened harbored AmpC enzymes, of which 43.75% of E.coli and 56.25% of Klebsiella isolates coproduced ESBL enzymes. Pure AmpC production was observed in 56.25% of E.coli and 43.75% of Klebsiella isolates. The inhibitor based test was useful in identifying cefoxitin susceptible AmpC producers and could also effectively differentiate ESBL from AmpC producing isolates.

Keywords: ESBL, antibiotic susceptibility, clinical samples, β-lactam disks.

Download full journal in PDF below

Detection of Amp C Beta Lactamases in Clinical Isolates of Escherichia coli and Klebsiella