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Nasal colonization of methicillin and glycopeptide resistant Staphylococcus aureus among outpatients at a clinic facility in Maputo, Mozambique: A cross-sectional study

*1,2Sineque, A., 1Mabasso, A. A., 1Chongo, A. E., and 1Mussagy,A.

 

1Department of Biological Sciences, Eduardo Mondlane University, Maputo, Mozambique

2Centre for Functional Ecology – One Health, Department of Life Sciences, University of Coimbra, Coimbra, Portugal *Correspondence to: sineque.alberto@uem.ac.mz; ORCID: https://orcid.org/0000–0003–2468–9925

 

Abstract:

 

Background: Nasal colonization by Staphylococcus aureus is a major source of community and hospital associated staphylococcal infections, including methicillin-resistant S. aureus (MRSA) infections. Screening of MRSA nasal colonization is important in the prevention and control of infection and may provide useful information to guide antimicrobial therapy. The study aimed to determine the prevalence of nasal colonization by methicillin-resistant S. aureus and its glycopeptide susceptibility pattern among symptomatic and asymptomatic individuals in Maputo, Mozambique. Continue reading “Nasal colonization of methicillin and glycopeptide resistant Staphylococcus aureus among outpatients at a clinic facility in Maputo, Mozambique: A cross-sectional study”

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Prevalence of mecA and vanA genes in nasal Staphylococcus aureus isolates from hospitalized patients in Babcock University Teaching Hospital, Ilishan-Remo, Nigeria

*¹Okunbor, H. N., ¹,²Otaigbe, I. I., ¹,²Oluwole, T. O., ¹,²Elikwu, C. J., and 3Bhola, A. K.

¹Department of Medical Microbiology, Babcock University Teaching Hospital, Ilishan-Remo, Ogun State, Nigeria

²Department of Medical Microbiology and Parasitology, Babcock University, Ilishan-Remo, Ogun State, Nigeria

³ISID-BMGF Research Capacity Building Mentor

*Correspondence to: okunborh@babcock.edu.ng; Tel: +2348059834262; ORCID: 0009-0001-2016-6310

Abstract:

Background: Antimicrobial resistance (AMR) among Staphylococcus aureus, particularly methicillin-resistant S. aureus (MRSA) and vancomycin-resistant S. aureus (VRSA), poses a major global public health challenge. Data on the molecular epidemiology of resistance genes in Nigeria remain limited. The objectives of this study are to determine the prevalence of mecA, vanA, and vanB genes among nasal S. aureus isolates, assess antimicrobial susceptibility profiles, and explore associations of prevalence with demographic and clinical characteristics of hospitalized patients in Babcock University Teaching Hospital (BUTH), Ilishan-Remo, Nigeria. Continue reading “Prevalence of mecA and vanA genes in nasal Staphylococcus aureus isolates from hospitalized patients in Babcock University Teaching Hospital, Ilishan-Remo, Nigeria”

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Multidrug-resistant Staphylococcus aureus associated with neonatal sepsis in the special care baby unit of a secondary healthcare facility in Katsina State, Nigeria

 

*1Bindawa, H. A., 2,3Abdulkadir, B., 2Rumah, H. M., and 4Samaila, A.

1College of Health Sciences and Technology, Katsina, Katsina State, Nigeria    2Department of Microbiology, Umaru Musa Yar’adua University, Katsina, Katsina State, Nigeria

3Medical Laboratory Science, Umaru Musa Yar’adua University, Katsina, Katsina State, Nigeria

4Department of Clinical Pharmacology and Therapeutics, College of Health Sciences,

Umaru Musa Yar’adua University, Katsina, Katsina State, Nigeria                            *Correspondence to: hafsatbindawa96@gmail.com

 

Abstract:

 

Background: Neonatal sepsis remains a major cause of morbidity and mortality in developing countries, with

Staphylococcus aureus being one of the leading causative organisms. The rising occurrence of multidrug-resistant (MDR) strains poses significant therapeutic challenges. This study aimed to determine the antibiotic resistance pattern and multiple antibiotic resistance (MAR) index of S. aureus isolated from neonatal sepsis cases in the Special Care Baby Unit (SCBU) of a secondary healthcare facility in Katsina State, Nigeria. Continue reading “Multidrug-resistant Staphylococcus aureus associated with neonatal sepsis in the special care baby unit of a secondary healthcare facility in Katsina State, Nigeria”

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Dual vanA/vanB gene-carrying vancomycin-resistant Staphylococcus aureus: An emerging threat from northeast Nigeria

*1Hamza, J. A., 2Garba, L., 2Yarma, A. A., 2Ibrahim, H. I., and 1Adamu, H.
1Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Gombe State University, Gombe, Nigeria 2Department of Microbiology, Gombe State University, Gombe, Nigeria *Correspondence to: jabirgsu@gsu.edu.ng, Te: +2347039306208

Abstract:
Background: Vancomycin is an antibiotic of last resort for the treatment of multidrug resistant Staphylococcus aureus including methicillin resistant S. aureus (MRSA). The emergence of vancomycin resistant S. aureus (VRSA) is a major threat to global healthcare with devastating implications. In Nigeria, data on VRSA is scarce while the molecular characterization of the organism from Northeast region is nearly absent. This current is aim to detect vancomycin resistance among MRSA isolates from Gombe State, Nigeria, and identify the genetic determinants involved. Continue reading “Dual vanA/vanB gene-carrying vancomycin-resistant Staphylococcus aureus: An emerging threat from northeast Nigeria”

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Efflux and mecA/mecC-mediated-resistance in multi-drug-resistant Staphylococcus aureus clinical isolates from selected Ghanaian primary and tertiary hospitals

1Zumbi, C. N., 2Odoi, H., *1Boamah, V. E., 1Boakye, Y. D., and 1Agyare, C.

1Pharmaceutical Microbiology Section, Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

2Department of Pharmaceutical Microbiology, School of Pharmacy, University of Health and Allied Sciences, Ho, Ghana

*Correspondence to: etsiapa@yahoo.com; veboamah.pharm@knust.edu.gh;

 

Abstract:

 Background: Globally, methicillin-resistant Staphylococcus aureus (MRSA) infections are associated with increased morbidity and mortality amongst patients; however, reports on MRSA infections in Ghana are mostly from tertiary healthcare facilities. This study determined the prevalence of methicillin and multiple efflux resistance mechanisms in S. aureus isolates from five selected primary and tertiary healthcare facilities in Ghana. Continue reading “Efflux and mecA/mecC-mediated-resistance in multi-drug-resistant Staphylococcus aureus clinical isolates from selected Ghanaian primary and tertiary hospitals”

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Bacteriological profile of Staphylococcus aureus isolates at the Bogodogo University Hospital Center, Ouagadougou, Burkina Faso

[1],[2]*Condé, M. S., [3],[4]Ky/Ba, A., 3,5Tondé, I., 4Savadogo, S. M., 4Ouattara, A., 4Yourgbarré, J., 4Ouédraogo, Y., 6Nagalo, Y. Y. A. A., 3Sanou, I., and 6,7Ouédraogo,A.

1Department of Biomedical Analysis Laboratory, Ignace Deen University Hospital, Conakry, Guinea

2Faculty of Health Sciences, Gamal Abdel Nasser University, Conakry, Guinea

3Laboratory of Bacteriology-Virology, UFR SDS, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso

4Department of Biomedical Analysis Laboratory, Bogodogo University Hospital, Ouagadougou, Burkina Faso

5Department of Biomedical Analysis Laboratory, Charles de Gaulle Pediatric University Hospital, Ouagadougou, Burkina Faso

6Laboratory of Emerging and Re-emerging Pathogens (LaPathER), Nazi BONI University, Bobo-Dioulasso 01 PO 676, Burkina Faso

7Bacteriology and Virology Service, Laboratory Department, Sourô Sanou University Hospital,  Bobo-Dioulasso 01 PO 676, Burkina Faso *Correspondence to: mamadisaran93@gmail.com; Tel: (226) 64645543

Abstract:

Background: Staphylococcus aureus is a common major human pathogen involved in a wide variety of infections. The objective of this study is to investigate the bacteriological profile of S. aureus at the Bogodogo University

Hospital Center, Ouagadougou, Burkina Faso.     Continue reading “Bacteriological profile of Staphylococcus aureus isolates at the Bogodogo University Hospital Center, Ouagadougou, Burkina Faso”

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Emergence of clinical vanA-type vancomycin-resistant Staphylococcus aureus isolates in National Orthopaedic Hospital Dala, Kano, Nigeria

*[1],3Abdulrahim, U., [2]Oche, D. A., [3]Kachallah, M., 4Adeshina, G. O., and 4Olayinka, B. O.

 1Department of Medical Microbiology, School of Basic Medical Sciences,              Central South University, Hunan Province, China

2Department of Microbiology, Faculty of Science, Federal University of               Health Sciences Otukpo, Benue State, Nigeria

3Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, University of Maiduguri, Borno State, Nigeria                                                  4Department of Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria                       *Correspondence to: uabdulrahim@csu.edu.cn and uabdulrahim@unimaid.edu.ng

Abstract:  

Background: The increasing prevalence of multi drug resistance (MDR) in strains of Staphylococcus aureus is a major challenge in the selection of an appropriate therapeutic agents, especially in persistent orthopaedic infections. This study investigated the patterns of antimicrobial resistance and identified the genetic determinants of resistance in S. aureus isolates from orthopaedic patients. Continue reading “Emergence of clinical vanA-type vancomycin-resistant Staphylococcus aureus isolates in National Orthopaedic Hospital Dala, Kano, Nigeria”

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Prevalence of Macrolide-Lincosamide-Streptogramin-B resistance among clinical Staphylococcus aureus isolates in University of Ilorin Teaching Hospital, Ilorin, Nigeria

*1,2Ade, T. I., 1Osiyemi, J. A., 2Aso, R. E., 3Akinduti, P. A., and 4Sunmola, N. O.
1Department of Medical Microbiology and Parasitology, University of Ilorin, Nigeria
2Department of Microbiology, Federal University Wukari, Taraba State, Nigeria
3Microbiology Unit, Department of Biological Sciences, Covenant University, Ota, Nigeria
4Department of Medical Microbiology and Parasitology, Olabisi Onabanjo University, Ogun State, Nigeria
*Correspondence to: tolulope.iorwuese@gmail.com; +2347066369670

Abstract:
Background: Inducible antibiotic resistance among Gram-positive cocci is a significant public health challenge that is grossly underreported within Africa, especially Nigeria. Hence, the aim of this study was to determine the prevalence of macrolide-lincosamide-streptogramin-B (MLSB) resistance among clinical isolates of Staphylococcus aureus at University of Ilorin Teaching Hospital, Ilorin, Nigeria.

Methodology: Clinical isolates were presumptively identified by Gram’s stain reaction and conventional biochemical tests such as catalase, coagulase, DNase, and mannitol fermentation. Phenotypic MLSB resistance was determined by placing clindamycin and erythromycin discs within 15 mm of each other and observing for a D-zone. Antibiotic sensitivity testing to selected antibiotics including cefoxitin for detection of methicillin resistance, was done using the modified Kirby-Bauer disc diffusion method. Continue reading “Prevalence of Macrolide-Lincosamide-Streptogramin-B resistance among clinical Staphylococcus aureus isolates in University of Ilorin Teaching Hospital, Ilorin, Nigeria”

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Characterization of biofilm formation in clinical urinary isolates of Staphylococcus aureus from five hospitals in Lagos State, Nigeria

 1Orjih, C. I., 2Ajayi, A., 1,3Alao, F. O., 1Adeleye, A. I., and *2,4Smith, S. I.  

 1Department of Microbiology, University of Lagos, Akoka, Nigeria

2Molecular Biology and Biotechnology Department, Nigerian Institute of Medical Research, Lagos, Nigeria

3Department of Biological Sciences, Bells University of Technology, Ota, Ogun State, Nigeria                       

4Mountain Top University, Lagos-Ibadan Expressway, Ogun State, Nigeria

*Correspondence to: stellaismith@yahoo.com                              

Abstract:                                                                                               

Background: Biofilm formation by pathogens is of great clinical importance as it mediates persistence and resistance to antibiotics, hence posing difficulty in treatment and management of diseases. The aim of this study was to evaluate the biofilm forming potential of Staphylococcus aureus isolated from urine samples of females with urinary tract infection and to detect the presence of clumping factor (clfA) and intracellular adhesion (icaA) encoding genes.                                                           

Methodology: A total of 50 S. aureus were obtained from urine samples of women in five hospitals in Lagos State, Nigeria. Isolates were confirmed by standard biochemical and novobiocin susceptibility tests. The isolates were screened for biofilm formation using three methods; Congo-red agar (CRA), tube, and tissue culture plate (TCP) methods. Detection of clfA and icaA genes was done by PCR.          Continue reading “Characterization of biofilm formation in clinical urinary isolates of Staphylococcus aureus from five hospitals in Lagos State, Nigeria”

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Effects of rinsing on Staphylococcus aureus load in frozen meats and fish obtained from open markets in Benin City, Nigeria

Ogofure, A. G., and *Igbinosa, E. O.

Applied Microbial Processes & Environmental Health Research Group (AMPEHREG), Department of Microbiology, Faculty of Life Sciences, University of Benin, Private Mail Bag 1154, Benin City 300283, Nigeria

*Correspondence to: etinosa.igbinosa@uniben.edu

Abstract:

Background: Staphylococcus aureus is a ubiquitous bacterium present in the environment and one of the leading causes of superficial and deep infections. In the food industry, it is acclaimed to be globally responsible for several food-borne diseases. This study was designed to isolate methicillin-resistant S. aureus (MRSA) and determine the effect of rinsing on MRSA load in frozen meat and fish obtained from open market in Benin City. Continue reading “Effects of rinsing on Staphylococcus aureus load in frozen meats and fish obtained from open markets in Benin City, Nigeria”