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Isolation and biocontrol of bacteriophages from wastewater in the city of Lomé, Togo: potential application as a novel source for antimicrobial therapy

1Ouedraogo, A. K., *1Hoekou, Y., 2Gbekley, H. E., 1Pissang, P., 1Kpatagnon K., 1Sossou, K., 2Melila, M., 3Djeri, B., and 1Tchacondo, T.

1Laboratory of Biomedical and Food Sciences and Environmental Health, Advanced School of Biological and Food Processing Techniques, Université de Lomé, Togo
2Faculty of Sciences, Laboratory of the Department of Biochemistry, Université de Lomé, Togo
3Laboratory of Microbiology and Food Quality Control, Advanced School of Biological and Food Processing Techniques, Université de Lomé, Togo

*Correspondence to: yhoekou@gmail.com; 0022890259591

Abstract:
Background: Bacteriophages offer one of the most promising solutions to the challenges of antimicrobial resistance in bacteria. The aim of this study is to investigate bacteriophages as a source of new antimicrobial therapy.

Methodology: Waste water samples were randomly collected from 8 different locations in the city of Lomé for bacteriophage isolation. The phages were isolated using multi-resistant clinical isolates (Escherichia coli 1642 and Staphylococcus aureus 0868) as hosts by means of a spot test. The host range of the phages was determined also by a spot test using 8 other clinical bacterial isolates including two reference strains (E. coli ATCC 25922 and S. aureus ATCC 29213). The virulence of the phages and their effects on bacterial growth were assessed by in vitro experiments using E. coli 1642 BBec phage suspension. Continue reading “Isolation and biocontrol of bacteriophages from wastewater in the city of Lomé, Togo: potential application as a novel source for antimicrobial therapy”

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High faecal carriage of extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-PE) among hospitalized patients at Sylvanus Olympio Teaching Hospital, Lomé, Togo in 2019

*1,2Godonou, A. M., 2Lack, F., 3,4Gbeasor-Komlanvi, F. A., 2Konlani, L., 2,3Dossim, S., 1Ameyapoh, Y. A., 3,4Ekouevi, K., 2,3Dagnra, A. Y., and 3,5Salou, M.

1High School of Biological and Food Technics, University of Lomé, Togo

2Teaching Hospital Sylvanus Olympio, Lomé, Togo

3Faculty of Health Sciences, University of Lomé, Togo

4Faculty of Health Sciences Public Heath Department, University of Lomé, Togo

5Teaching Hospital Campus, Lomé, Togo *Correspondence to: godonouvirginie@yahoo.fr

Abstract:
Background: Extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-PE) are a global health concern, associated with increased morbidity and mortality. Even in the absence of infections, colonization by these pathogens is still a great threat because of the risk of cross transfer among hospitalized patients. Faecal carriage of ESBL-PE remained poorly documented in Africa. This study aimed to determine faecal carriage rate of ESBL-PE, factors associated with carriage, and antimicrobial susceptibility of the strains among hospitalized patients at Sylvanus Olympio Teaching Hospitals (CHU SO) in Lomé, Togo. Continue reading “High faecal carriage of extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-PE) among hospitalized patients at Sylvanus Olympio Teaching Hospital, Lomé, Togo in 2019”

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Faecal carriage of multi-drug resistant Enterobacteriaceae in hospitalized children at University Teaching Hospital Sylvanus Olympio of Lomé, Togo

1Lack, F., 1Tsogbalé, A., 2Doumegno, J. K., 3Dossim, S., 1Dagnra, A., and *1,4Salou, M.

1Laboratory of Molecular Biology and Immunology, Faculty of Health Sciences, University of Lomé, Lomé, Togo

2Laboratory of Microbiology and Food Quality Control, University of Lomé, Togo

3CHU Kara Medical Biology Laboratory, Kara, Togo

4National AMR Reference Laboratory CHU Campus, Lomé, Togo

*Correspondence to: mounerous@gmail.com

Abstract:
Background: High prevalence of infections and associated antibiotherapy may put children at increased risk for development of multidrug-resistance (MDR), mostly to bacterial infections. The objective of this study therefore was to determine the prevalence of gastrointestinal carriage of MDR Enterobacteriaceae among hospitalized children in the Paediatric department of Sylvanus Olympio University Hospital, Lomé, Togo. Continue reading “Faecal carriage of multi-drug resistant Enterobacteriaceae in hospitalized children at University Teaching Hospital Sylvanus Olympio of Lomé, Togo”