Prabu, R., Prasad, S. V. S., Kodimule, S., and *Sunhare, R.Development and Research Centre-Probiotics, Vidya Herbs Pvt Ltd, No. 102B & 105B, Pharmaceuticals SEZ
Industrial area, KIDB, Hassan, 573201 Karnataka, India
*Correspondence to: raksha@vidyaherbs.com; +91-8600040075
Abstract:
Background: Probiotics alter gut microbiota composition and diversity. Research indicates that probiotic supplementation might enhance glucose homeostasis and reduce inflammation in patients with type Ⅱ diabetes mellitus. The aim of this study was to investigate how particular probiotic strains (VHBAX Bacillus coagulans) affect glucose homeostasis and appetite-related behaviours associated with metabolic illnesses such as obesity and type Ⅱ diabetes in a zebrafish model.
Methodology: An experimental laboratory-based investigation was carried out with zebrafish (Danio rerio) as the model organism. The zebrafish were divided into 3 groups of 6 zebrafish each; control (group I), diabeticinduced (group II), and diabetic-induced plus probiotic treatment (group III), with the latter receiving a diet supplemented with VHBAX probiotic strains. Glucose tolerance testing, and gene expression analysis for glucose metabolism were carried out in all the 3 groups. The composition of the gut microbiota was also analysed using 16S rRNA sequencing to assess microbial alterations related to probiotic administration. Data were analysed using SPSS 16.0 and presented as mean±SD. Difference between means of two groups was determined by Student ttest, and p<0.05 was considered statistically significant.
Results: When compared to diabetes-induced zebrafish (group II), diabetes-induced probiotic-treated zebrafish (group III) had enhanced glucose tolerance, altered expressions of appetite-regulating genes (AgRP, CNR1, CTSL1, Oxt, CCKAr, leptin A), and detectable alterations in gut microbiota composition, comparable to the control (group I). There was a significant association of VHBAX probiotic supplementation with microbial regulation and improved metabolic outcomes.
Conclusion: This experimental investigation shed light on the mechanisms by which VHBAX probiotics regulate glucose metabolism and appetite management through alteration of gut microbiota composition in zebrafish. The finding suggests that probiotic therapies can help manage or prevent metabolic disorders, including obesity and type Ⅱdiabetes.
Keywords: Zebra fish, Glucose metabolism, Diabetes, Microbiota, Metabolic disorder, Probiotics
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