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Bloodstream infection caused by Kocuria species in a tertiary hospital in Lagos, Nigeria: A case series

*1Osuagwu, C. S., 2,3Davies, A. A., and 1Oladele R. O.

1Department of Medical Microbiology and Parasitology, Lagos University Teaching Hospital, Idi-Araba, Lagos, Nigeria

 2Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria

3Department of Medical Microbiology and Parasitology, Olabisi University Teaching Hospital, Sagamu, Nigeria

*Correspondence to: chiomaosuagwu@gmail.com

Abstract

Kocuria species are emerging opportunistic pathogens commonly misidentified as coagulase-negative staphylo- coccus. Improvement in laboratory diagnostic techniques and the expansion in the number of patients with immunocompromised diseases has led to the rising number of Kocuria infections being reported. We report cases of Kocuria bacteraemia in three immunocompromised patients. The first case was an 11-year-old with metastatic nasopharyngeal carcinoma and Kocuria kristinae isolated from the second blood culture, but due to discharge of the patient against medical advice, the role of K. kristinae and outcome of the case could not be ascertained. The second case was a 30-year-old with acute lymphoblastic leukaemia, with fever and diarrhoea, and Kocuria species isolated from the second blood culture, but despite treatment with antibiotic with invitro activity against the organism, the outcome was fatal. The third case was a very low birth weight premature male neonate who was delivered by emergency cesarean at 31 weeks gestation, due to maternal chronic hypertension and superimposed preeclampsia. Although, Kocuria rosea was isolated from the third blood culture of the case, it was most likely a colonizer, as the clinical condition of the patient had improved before isolating the organism. This report may be the first case of bacteraemia caused by Kocuria from Nigeria, indicating that this organism should not ignored as a possible cause of bacteraemia because advancement in laboratory diagnostics will aid correct identification. Continue reading “Bloodstream infection caused by Kocuria species in a tertiary hospital in Lagos, Nigeria: A case series”

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Short  Communication Open Access Antibiotic susceptibility of bacteria isolated from suppurative acute otitis media in children in Ouagadougou, Burkina Faso  

[1],3Tondé, I., [2]Guiguemdé, K. T., *[3],[4]Condé, M. S., 2Savadogo, P. M., 2,3Sanogo, Z., 1,3Tapsoba, A., 3Tamboura, M., 2,3Soma, W., 1Béré, P. L., 3Somé, C. K., 1Ky/Ba, A., 1Sanou, I., and [5]Gyebré, Y. M. C.

1Laboratory of Bacteriology-Virology, UFR SDS, Joseph Ki-Zerbo Université, Ouagadougou, Burkina Faso

2Laboratory of Parasitology-Mycology, UFR SDS, Joseph Ki-Zerbo Université, Ouagadougou, Burkina Faso

3Department of Biomedical Analysis Laboratory, Charles de Gaulle Pediatric University Hospital, Ouagadougou, Burkina Faso

4Department of Biomedical Analysis Laboratory, Ignace Deen University Hospital, Conakry Guinea

5Department of Otolaryngology, Yalgado Ouédraogo University Hospital, Ouagadougou, Burkina Faso *Correspondence to: mamadisaran93@gmail.com; Tel: (226) 64645543

Abstract:

Background: Acute otitis media (AOM) is one of the most common bacterial infections in children. The emergence of antimicrobial resistance (AMR) poses a threat to the management of these infections. The objective of this study was to identify and describe the sensitivity profile of bacterial agents isolated in AOM to commonly used antibiotics in the city of Ouagadougou, Burkina Faso.                           

Methodology: This was a descriptive cross-sectional study conducted between September 2023 and February 2024 in children aged 0-15 years with clinical features of suppurative AOM seen at the otorhinolaryngology (ORL) departments of four health facilities in Ouagadougou. Ear discharge was collected using sterile swabs and cultured on Chocolate agar media, incubated at 37°C for 24-48 hours in 5% CO₂. The sensitivity of the isolated bacteria to antibiotics was determined using the Kirby-Bauer disc diffusion method and interpreted according to the 2021 guideline of the Antibiogram Committee of the French Society of Microbiology (CA-SFM).

Results: A total of 61 children with AOM were recruited and bacteriological culture examination was performed on the ear swabs obtained from them. All but two cultures were positive. A total of 59 bacterial pathogens were isolated with Gram-negative bacilli (GNB) more commonly isolated (67.8%, n=40) in the children, dominated by Klebsiella pneumoniae (18.6%, n=11) and Pseudomonas aeruginosa (18.6%, n=11). Aztreonam and imipenem were the most active antibiotics against GNB, but majority of the GNB were sensitive to gentamicin and ciprofloxacin.                          

Conclusion: Acute otitis media in children, combined with the growing resistance of pathogens to antibiotics, is a real public health challenge. It is necessary to strengthen regular microbiological monitoring and the development of appropriate therapeutic strategies in order to limit the emergence and spread of resistant strains.

Keywords: Acute otitis media, AMR, Antibiotics, Bacteria, Burkina Faso

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Evaluation of microbial quality of grilled foods sold at roadside in Calabar metropolis, Cross River State, Nigeria

*1Inyang, A. N., 2Agiang, M. A., 2Mgbang, J. E., 2Arikpo, O. O., 2Ottoho, E. O., and 2Eze, D. E.

1Department of Biochemistry, Arthur Jarvis University, Akpabuyo, Nigeria

2Department of Biochemistry, University of Calabar, Calabar, Nigeria

*Correspondence to: aniekemeinyang39@gmail.com; Mobile: +234(0)813 566 8258

Abstract:

Background: Roadside foods are central parts of culinary custom in many African countries. In addition to their dietary importance, they contribute immensely to the national and economic values of a nation. However, the growing incidence of food-borne illnesses in relation to the roadside foods is a great concern. Hence, this study assessed the microbial quality of grilled foods sold at roadside in Calabar metropolis, Cross River State, Nigeria.      Continue reading “Evaluation of microbial quality of grilled foods sold at roadside in Calabar metropolis, Cross River State, Nigeria”

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Prevalence and antibiotic susceptibility of acute bacterial meningitis isolates among children with fever and convulsion in Federal Medical Centre, Bida, Nigeria

[1]Oyeleye, A. E., 1Afeez, B. O., 1Ayanbeku, T. S., and *2Ubesie, A. C.

 1Department of Paediatrics, Federal Medical Centre, Bida, Niger State, Nigeria   2Department of Paediatrics, College of Medicine, University of Nigeria, Enugu State, Nigeria

*Correspondence to: agozie.ubesie@unn.edu.ng

Abstract:

Background: Acute bacterial meningitis (ABM) is a life-threatening illness associated with high morbidity and mortality as well as debilitating consequences among survivors. The objective of this study is to determine the prevalence of bacterial isolates and antibiotic susceptibility pattern of ABM among children presenting with fever and convulsion at the Federal Medical Centre, Bida, Nigeria.         Continue reading “Prevalence and antibiotic susceptibility of acute bacterial meningitis isolates among children with fever and convulsion in Federal Medical Centre, Bida, Nigeria”

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Molecular epidemiology of genital Chlamydia trachomatis infections among women of reproductive age living with HIV/AIDS in a Nigerian tertiary health institution

*1,2,3Abayomi, S. A., 3Akanbi, A. A., 2Taiwo, S. S., 4Omoare, A. A., and 5Adegboro, B.

 1Cancer Research UK, Swansea, Wales, United Kingdom                                                2Department of Medical Microbiology and Parasitology, LAUTECH Teaching Hospital, Ogbomoso, Nigeria

3Department of Medical Microbiology and Parasitology, University of Ilorin Teaching Hospital, Ilorin, Nigeria

4Public Health Laboratory Services, Nigeria Centre for Disease Control and Prevention (NCDC), Abuja, Nigeria

5Department of Medical Microbiology and Immunology, Nile University of Nigeria, Abuja, Nigeria  *Correspondence to: subslaabayomi@gmail.com

Abstract:

Background: Chlamydia trachomatis is the most prevalent bacterial pathogen causing sexually transmitted infections (STIs) globally. This study investigates the genovars of genital C. trachomatis infection among women of reproductive age living with HIV/AIDS in Ilorin, Nigeria.      Continue reading “Molecular epidemiology of genital Chlamydia trachomatis infections among women of reproductive age living with HIV/AIDS in a Nigerian tertiary health institution”

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Antibiotic resistance profile of Non-Fermenting Gramnegative bacteria recovered from clinical specimens in a tertiary care teaching hospital, Hyderabad, India

*Kumuda, A., and Pavani, G.

Department of Microbiology, Apollo Institute of Medical Sciences and Research, Hyderabad, India *Correspondence to: kumudarumugam@gmail.com; +918805647838

 Abstract

 Background: Non-Fermenting Gram-negative bacilli (NFGNB), including Pseudomonas aeruginosa, Acinetobacter species, and Burkholderia species, are emerging as significant opportunistic pathogens, especially in immunocompromised and hospitalized patients. These organisms pose a major therapeutic challenge due to their multidrug-resistance (MDR) and intrinsic resistance mechanisms. The goal of this study is to determine antibiotic susceptibility pattern of NFGNB isolated from clinical specimens of patients in a tertiary care teaching hospital in Hyderabad, India.

Methodology: A descriptive cross-sectional observational study of patients with clinical infections was conducted between December 2024 and April 2025, to identify NFGNB from their clinical specimens. Different clinical samples (blood, sputum, urine, pus and others) were collected from patients of both sexes and all age groups for bacterial isolation using automated Bact/Alert for blood culture and conventional method for other samples. Identification of NFGNB isolates and antimicrobial susceptibility testing (AST) of the isolates against selected antibiotics were performed using the VITEK 2 Compact system, and AST interpretations were made in line with the Clinical and Laboratory Standards Institute (CLSI) guidelines. Continue reading “Antibiotic resistance profile of Non-Fermenting Gramnegative bacteria recovered from clinical specimens in a tertiary care teaching hospital, Hyderabad, India”

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Phenotypic identification and species characterization of clinically significant coagulase-negative staphylococci isolates at Bouaké University Hospital, Côte d’Ivoire

*1,2N’Guessan, A. M., 1,2Monemo, P., 1Traoré, A., 1,2Tadet, J. O. N., 1,2Gawa, K. J.,

1Ouattara, A., and 1,2Akoua Koffi, C.

1Bacteriology-Virology Laboratory, Bouaké University Hospital Center, Ivory Coast      r2Faculty of Medical Sciences, Alassane Ouattara University, Ivory Coast

*Correspondence to: mienmooh@gmail.com; +2250707669820

 

Abstract:

 Background: Coagulase-negative staphylococci (CoNS) are now recognized as important agents of nosocomial infections, particularly in immunocompromised patients in the healthcare settings, due to their ability to form biofilms and their high resistance to methicillin. The aim of this study is to identify and characterize different species of CoNS isolated at the Bacteriology-Virology Laboratory of the Bouaké University Hospital, Côte d’Ivoire Continue reading “Phenotypic identification and species characterization of clinically significant coagulase-negative staphylococci isolates at Bouaké University Hospital, Côte d’Ivoire”

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Comparative evaluation of GenoType MTBDRplus line probe assay and solid culture for detection of drug-resistant tuberculosis in northcentral Nigeria

*1,[1]Eliya, T. T., 1,2Ajide, B. A., 1Bassey, E., 1,2Ishaku, P., 1,5Adeyanju, B. D., 1Jonathan, K, and 1,[2],[3]John, B.

1Zankli Research Centre, Bingham University Karu, Km 26 Abuja-Keffi Expressway, Karu, Nigeria

2Biological Sciences Department, Bingham University Karu, Km 26 Abuja-Keffi Expressway, Karu, Nigeria

3Clinical Sciences, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, United Kingdom

4Community Medicine, Bingham University Karu, Km 26 Abuja-Keffi Expressway, Karu, Nigeria

[4]Institute of Human Virology, Plot 62 Emeritus Zone, Abuja, Nigeria

*Correspondence to: iliya.taryuta@binghamuni.edu.ng; +2347034229746

 

Abstract:

 Background: Multi-drug resistant tuberculosis (MDR-TB) poses a great threat to the TB control programs worldwide. Early detection of rifampicin (RIF) and isoniazid (INH)-resistant Mycobacterium tuberculosis (MTB) is essential for effective treatment and control of MDR-TB. This study evaluated the performance of GenoType MTBDRplus Line Probe Assay (LPA) with the solid culture method for early diagnosis of MDR-TB.   Continue reading “Comparative evaluation of GenoType MTBDRplus line probe assay and solid culture for detection of drug-resistant tuberculosis in northcentral Nigeria”

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Prevalence and predictors of hepatitis B screening among pregnant women attending antenatal clinics in three primary health centres in Abeokuta, Nigeria: A cross-sectional study

*Aliu, O. O., Fowotade, A., and Omoruyi, C. E.

Department of Medical Microbiology, University College Hospital (UCH), Ibadan, Nigeria

*Correspondence to: aliu_my2004@yahoo.com

 

Abstract:

 Background: Hepatitis B virus (HBV) infection remains a major public‑health concern. Antenatal screening identifies maternal infection and enables interventions to prevent mother‑to‑child transmission. We assessed the prevalence and predictors of prior HBV screening among pregnant women attending three primary health centres in southwest Nigeria.

Methodology: This was a cross‑sectional survey of 289 pregnant women consecutively enrolled at the antenatal clinics of the three selected health centres. A standardized questionnaire was interviewer-administered to each participant to collect information on socio‑demographic characteristics, previous HBV screening and behavioral and healthcare‑ factors related to HBV screening. Data were analysed using SPSS. Associations of factors with prior HBV screening were determined using Chi‑square and multivariable logistic regression estimated adjusted Odd ratio (AOR) with 95% confidence interval (CI). Continue reading “Prevalence and predictors of hepatitis B screening among pregnant women attending antenatal clinics in three primary health centres in Abeokuta, Nigeria: A cross-sectional study”