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Molecular characterization of Cryptosporidium spp from stool samples of people living with human immunodeficiency virus in Maiduguri, Nigeria

*1,[1]Kadaura, M. U., 1Abubakar, J., 2Baba, A. S., 3Askira, M. U., 2Ali, K. B., 4Yakubu, Y. M., 4Daggash, B. B., 4Baba, S. A., 5Mohammed, Y., 2Gadzama, G. B., and 4Zailani, S. B.

1Department of Medical Microbiology and Parasitology, Faculty of Basic Clinical Sciences, Federal University of    Health Sciences Azare, Bauchi State, Nigeria

2Department of Medical Microbiology and Parasitology, Faculty of Basic Clinical Sciences, University of Maiduguri, Borno State, Nigeria

3Department of Medical Laboratory Science, Faculty of Allied Health Sciences, University of Maiduguri, Borno State, Nigeria

4Department of Medical Microbiology and Parasitology, University of Maiduguri Teaching Hospital,  Maiduguri, Borno State, Nigeria                          5Department of Medical Microbiology and Parasitology, Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria

*Correspondence to: Umulsan4maryam@gmail.com, Mairo.kadaura@fuhsa.edu.ng,.

Abstract:

Background: Cryptosporidium is an important cause of diarrhea in immuno-compromised individuals including children. Recent advances in molecular techniques have led to the discovery of different species that are more commonly associated with diarrhea. This study aimed at isolating and characterizing the Cryptosporidium spp among HIV patients with diarrhea in Maiduguri, Nigeria. Continue reading “Molecular characterization of Cryptosporidium spp from stool samples of people living with human immunodeficiency virus in Maiduguri, Nigeria”

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Improved Cryptosporidium case findings using immunofluorescent microscopy on concentrated stool

Cox, D., and *Robberts, F. J. L.

Division of Medical Microbiology, University of Cape Town, 1 Anzio Road, Observatory, 7925, South Africa *Correspondence to: lrobberts@gmail.com; +27 645256360; https://orcid.org/0000-0003-4397-4852

Abstract:
Background: Diarrhoea is a major cause of morbidity in Cape Town, South Africa, and mortality is attributed to a failure to recognize the severity of the condition. Cryptosporidium and Giardia are increasingly recognized as important causes of diarrhoea in Africa however, suboptimal diagnostic techniques may lead to underappreciation of their significance. Our objectives are to compare the diagnostic yield of direct immunofluorescent antigen (DFA) microscopy on concentrated stool samples for Cryptosporidium and Giardia, with the current approach of wet mount microscopy for Giardia and auramine fluorescent stain for Cryptosporidium on unconcentrated stool. Continue reading “Improved Cryptosporidium case findings using immunofluorescent microscopy on concentrated stool”