intensive care unit – afrjcem.org

*¹Sanusi, A. A., ^2,3Okesola, A. O., and ^2,4Odusanya, O. O.

¹Department of Anaesthesia, College of Medicine, University of Ibadan/University College Hospital, Ibadan, Nigeria

²Department of Medical Microbiology, College of Medicine, University of Ibadan, Ibadan, Nigeria

³Department of Medical Microbiology University College Hospital, Ibadan, Nigeria

⁴AllTalentz LLC

*Correspondence to: arinolasanusi@yahoo.com; +2348033250788; ORCiD number: 00000003-1725-9097

Abstract:

Background: Healthcare associated infections (HAIs), also known as nosocomial infections, pose a major safety risk for both patients and healthcare workers globally. Acinetobacter species are Gram-negative bacteria primarily associated with HAIs and have been implicated in outbreaks of infections such as ventilator-associated pneumonia, urinary tract infections, and septicaemia. The multi-drug resistance (MDR) nature of Acinetobacter species coupled with ability to survive for long periods in harsh conditions have made them of global health concern. Patients in intensive care units (ICUs) are highly susceptible to Acinetobacter infection. This study was designed to determine the prevalence and antibiotic resistance profiles of Acinetobacter isolates in infections among patients in the ICU of the University College Hospital, Ibadan, Nigeria. Continue reading “Open Access Acinetobacter baumannii infections among patients in intensive care unit at the University College Hospital, Ibadan, Nigeria”

E.M. EL-Behedy, M Hend, MM EL-Arini, M.A. Gerges, N.A.E. Mohamed, N.M. Gamil

Abstract

Non-fermentative Gram-negative bacilli (NFGNB) other than Pseudomonas and Acinetobacter species have emerged as nosocomial pathogens. No much data is currently available concerning the occurrence of these types of bacteria in Zagazig University Hospitals (ZUHs). In this study, the occurrence as well as the antimicrobial susceptibility pattern of unusual NFGNB obtained from clinical samples collected from intensive care units (ICUs) of ZUHs was assessed. Additionally, the genetic relatedness among the most prevalent unusual NFGNB species was studied.

Results: Out of 516 non-repeated clinical sample, 97 NFGNB (18.7%) were isolated. Among them, 17 unusual NFGNB were identified by API 20NE, accounting for 17.5% of NFGNB and 3.3% of all tested samples. Within the unusual NFGNB, Burkholderiacepaciacomplex (Bcc) was the most prevalent species accounting for 94.1% of NFGNB and 3.1% of total samples. This was followed by Burkholderia pseudomallei (B. pseudomallei) which accounted for 5.9% of NFGNB and 0.2% of all obtained specimens. Tigecycline antibiotic was the most effective antibiotic against Bcc isolates (68.8% susceptibility) in disc diffusion method. After random amplified polymorphic DNA (RAPD) testing, the obtained Bcc isolates were found to be genetically diverse. This highlights Bcc as an emerging nosocomial pathogen in ICUs of ZUHs. Continuous monitoring of the occurrence of Bcc in ICU as well as in other hospital wards is warranted.

Keywords: Unusual, Non-fermentative, gram-negative bacilli, intensive care unit

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Occurrence of unusual non-fermentative gram negative bacilli in intensive care units of a university hospital, Egypt