*1Odeyemi, O., and 2Enweani-Nwokelo, I. B.
1Faculty of Medical Laboratory Science, Achievers University, Owo, Nigeria
2Faculty of Medical Laboratory Sciences, Nnamdi Azikiwe University, Awka, Nigeria
*Correspondence to: yemiode@yahoo.com; Tel: +2347032971869
Abstract:
Background: Dermatophytosis is a highly contagious superficial mycosis of the genera Trichophyton, Epidermophyton and Microsporum that affects both man and animal. The different plant parts of Nauclea latifolia are known to have ethnomedicinal uses. The objective of this study is to access the phytochemical components of N. latifolia plant parts and to investigate the anti-fungal activities and time-kill kinetics of the plant parts against isolated dermatophyte species.
Methodology: The pulverized parts (root, stem, leaf, fruit) of authenticated N. latifolia plant were extracted using cold maceration method for 48 hours in methanol. The extracts were then concentrated almost to dryness and the phytochemical constituents assessed. The preliminary screening of the anti-dermatophytic activities of the extracts was determined by the agar disc diffusion technique on selected dermatophytes, consisting of two isolates of Microsporum ferrugineum (strains 1 and 2), two isolates of Trichophyton mentagrophytes (strains 1 and 2), one isolate of Microsporum audouinii and one isolate of Microsporum canis. The minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and time-kill kinetics of the extracts were determined using standard methods on the selected dermatophytes. Continue reading “Phytochemical screening, in vitro anti-fungal activities, and time-kill kinetics of methanol extracts of Nauclea latifolia against selected dermatophytes”