*Kumuda, A., and Pavani, G.
Department of Microbiology, Apollo Institute of Medical Sciences and Research, Hyderabad, India *Correspondence to: kumudarumugam@gmail.com; +918805647838
Abstract
Background: Non-Fermenting Gram-negative bacilli (NFGNB), including Pseudomonas aeruginosa, Acinetobacter species, and Burkholderia species, are emerging as significant opportunistic pathogens, especially in immunocompromised and hospitalized patients. These organisms pose a major therapeutic challenge due to their multidrug-resistance (MDR) and intrinsic resistance mechanisms. The goal of this study is to determine antibiotic susceptibility pattern of NFGNB isolated from clinical specimens of patients in a tertiary care teaching hospital in Hyderabad, India.
Methodology: A descriptive cross-sectional observational study of patients with clinical infections was conducted between December 2024 and April 2025, to identify NFGNB from their clinical specimens. Different clinical samples (blood, sputum, urine, pus and others) were collected from patients of both sexes and all age groups for bacterial isolation using automated Bact/Alert for blood culture and conventional method for other samples. Identification of NFGNB isolates and antimicrobial susceptibility testing (AST) of the isolates against selected antibiotics were performed using the VITEK 2 Compact system, and AST interpretations were made in line with the Clinical and Laboratory Standards Institute (CLSI) guidelines. Continue reading “Antibiotic resistance profile of Non-Fermenting Gramnegative bacteria recovered from clinical specimens in a tertiary care teaching hospital, Hyderabad, India”