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The role of 16S rRNA sequencing in bridging phenotypic– genotypic gaps in Salmonella identification: Evidence from a Nigerian endemic zone

*1Attah, C. J., 2Egah, D. Z., and 3Lar, P. M.

1Infectious Diseases Unit, Department of Paediatrics, Federal Medical Centre, Keffi, Nasarawa State, Nigeria                                

2Department of Medical Microbiology, Jos University Teaching Hospital, Jos, Plateau State, Nigeria

3Department of Microbiology, University of Jos, Jos, Plateau State, Nigeria

*Correspondence to: marysolspecialist2@gmail.com; attah.caleb@yahoo.com; +234 8069231690;

ORCID: 0000-0002-3261-1127

Abstract:

Background: Accurate laboratory identification of Salmonella species is essential for effective diagnosis, anti- microbial stewardship, and surveillance of enteric fever. Conventional culture and biochemical methods remain the primary diagnostic approach in many laboratories in resource-limited countries but are prone to misidentification because several members of the family Enterobacteriaceae exhibit similar phenotypic characteristics. This study evaluated the role of 16S rRNA gene sequencing in validating phenotypic identification of Salmonella isolates obtained from patients with suspected enteric fever in Nigeria. Continue reading “The role of 16S rRNA sequencing in bridging phenotypic– genotypic gaps in Salmonella identification: Evidence from a Nigerian endemic zone”

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Genomic characterization of an extensively drug-resistant Mycobacterium tuberculosis clinical isolate

*[1]Danjuma, I., [2]Timan, T. E., 1Daikwo, M. A., and 1Anunobi, O. O.

1Department of Biochemistry, Bingham University, Karu, Nasarawa State, Nigeria

 2Zankli Research Center, Bingham University, Karu, Nasarawa State, Nigeria

*Correspondence to: idanjuma4real@gmail.com; +2348063471293

Abstract:

Background: Tuberculosis (TB), caused by Mycobacterium tuberculosis, constitutes a predominant infectious disease with high mortality on a global scale, especially in Nigeria. The emergence of extensively drug-resistant (XDR) TB poses a significant challenge to the control of this disease, underscoring the necessity for the identification of underlying genetic mutations to facilitate informed treatment decisions and mitigate transmission. Continue reading “Genomic characterization of an extensively drug-resistant Mycobacterium tuberculosis clinical isolate”