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Phenotypic and molecular detection of methicillin-resistance and virulence factors in coagulase-negative staphylococci isolated from patients with urinary tract infections in southeastern Nigeria

*1Ikeagwu, I. J., 2,3Oshim, I. O., 4Obi, C. M., 5Odeyemi, O., 4Urama, E. U., 6Ikeagwulonu, C. R., 2,7Azi, S. O., 2,7Aniokete, U. C., 4Okeke-Nwolisa, B. C., and 2,3Okekpa,S. I.

1Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Nigeria

2Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, David Umahi Federal University of Health Science, Uburu, Ebonyi State, Nigeria

3International Institute for Oncology and Cancer, Uburu, Ebonyi State, Nigeria

4Department of Medical Microbiology and Public Health, Faculty of Medical Laboratory Science, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Anambra State, Nigeria

5Department of Medical Microbiology and Parasitology, Faculty of Medical Laboratory Science, Achiever University, Owo, Ondo State, Nigeria

6Department of Medical Laboratory Science, Alex Ekwueme Federal University Teaching Hospital Abakaliki, Ebonyi State, Nigeria

7International Institute of Infectious Disease, Biosafety and Biosecurity, Uburu, Ebonyi State, Nigeria

*Correspondence to: judithfmc@gmail.com

Abstract:

Background: The emergence of antibiotic resistance among coagulase-negative staphylococci (CoNS) is an important factor in nosocomial infections outcomes in the healthcare system. This study aimed to phenotypically detect methicillin resistance and genotypically detect mecA gene and other virulence markers in coagulasenegative staphylococci isolated from patients with suspected urinary tract infection (UTI) in Alex Ekwueme Federal University Teaching Hospital (AE-FUTHA), Abakaliki, Ebonyi State, Nigeria. Continue reading “Phenotypic and molecular detection of methicillin-resistance and virulence factors in coagulase-negative staphylococci isolated from patients with urinary tract infections in southeastern Nigeria”

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Genotypic identification of coliforms isolated from cases of subclinical mastitis among pastoral herds in parts of Kaduna State, Nigeria

1, 2*Makolo, D., 2Suleiman, A. B., 2Olonitola, O. S., 3Bello, M., and 4Ahmadu, I.

1Department of Sciences, School of Preliminary Studies, Kogi State Polytechnic, Lokoja, Nigeria

2Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Nigeria

3Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria

4National Tuberculosis Reference Laboratory, Zaria, Nigeria *Correspondence to: makolodaniel@gmail.com

Abstract:

Background: Mastitis caused by Staphylococcus aureus was initially considered the major problem in dairy herds, but over the last few decades, the incidence of coliform mastitis has increased among the pastoral herds in Nigeria due to poor environmental and milking hygiene. Hence, this study was aimed at genotypic identification of coliform bacteria isolated from cases of bovine mastitis among pastoral herds in parts of Kaduna State, Nigeria.

Methods: A cross-sectional survey of 30 herds of cows across 7 Local Government Areas of Kaduna State, Nigeria, was conducted. One hundred and forty seven cows were proportionately selected by purposive sampling technique. The milk samples were aseptically collected and bacteriologically screened for coliform bacteria following standard bacteriological techniques. Nine out of 12 coliforms identified phenotypically were selected for PCR amplification and sequencing of their 16S rRNA gene. The Basic Local Alignment Search Tool (BLAST) analysis of the sequences obtained was done on the National Centre for Biotechnology Information (NCBI) data base, and isolates confirmed based on similarity to 16S rDNA sequences in the Gen Bank Continue reading “Genotypic identification of coliforms isolated from cases of subclinical mastitis among pastoral herds in parts of Kaduna State, Nigeria”