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Prevalence, molecular characteristics and antibiotic susceptibility of clinical isolates of Clostridioides difficile in southeastern Nigeria

*1,2Oghonyon, 1,3Ugwu, M. C., 1,3Esimone, C. O., and 2Onah, A. I.

 [1]Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University Awka, Anambra State, Nigeria

[2]Department of Pharmaceutical Microbiology and Biotechnology, David Umahi Federal University of Health Sciences, Uburu, Ebonyi State, Nigeria

[3]TETFUND Centre of Excellence for Biomedical, Engineering and Agricultural Translational studies, Nnamdi

Azikiwe University Awka, Anambra State, Nigeria

*Correspondence to: oghonyonei@dufuhs.edu.ng, eddisonioghonyon@gmail.com; +2348038469094

 

Abstract:

 Background: Clostridioides difficile is a major contributor to healthcare-associated infections, exhibiting global variations in its prevalence and resistance. There is an absence of data on its molecular characteristics and antibiotic susceptibility of C. difficile in southeastern Nigeria. This study aims to evaluate its prevalence, molecular features, and resistance patterns to enhance treatment and control strategies.  Continue reading “Prevalence, molecular characteristics and antibiotic susceptibility of clinical isolates of Clostridioides difficile in southeastern Nigeria”

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Biochemical evaluation of liver function enzymes in Lassa fever patients

[1]Anjorin, A. A., *1Salami, W. O., 2Omojola, T. E., 1Ajoseh, S. O., 3Gbenga-Ayeni, B. O., 3Etafo, J., 1Lawal-Sanni, A. O., and 2,3Hassan, A. O.

1Department of Microbiology, Faculty of Science, Lagos State University, P. O. Box 0001, LASU Post Office, Ojo, Lagos, Nigeria

2Department of Medical Microbiology and Parasitology, Faculty of Medical Laboratory Science, Achievers University, Owo, Nigeria

3Infection Control and Research Laboratory, Federal Medical Centre, Owo, Nigeria

*Correspondence to: wasiu.salami@lasu.edu.ng; +2348032582702

 

Abstract:

 Background: Lassa fever (LF) is a zoonotic acute viral hemorrhagic disease caused by the Lassa virus (LV). It has a high case fatality rate of about 5,000 deaths in humans from 300,000-500,000 cases each year globally. The high mortality rate from Lassa fever has been associated with abnormal liver enzyme production due to LV infiltration of the liver. However, data are sparse on the different enzymes and their proportions associated with LF. This study aims to evaluate the effect of LF on the levels of selected liver enzymes. Continue reading “Biochemical evaluation of liver function enzymes in Lassa fever patients”

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Pathogenicity of filamentous fungi isolated from poultry farms on gastrointestinal system of day-old chicks in Anambra State, Nigeria

Mba, Anthonia Nkiruka

International Institute for Infectious Diseases, Biosafety and Biosecurity Research, David Umahi Federal University of Health Sciences, Uburu, Ebonyi State, Nigeria

Abstract:

Background: The pervasive presence of filamentous fungi in poultry farming environments has a profound impact on the bird health, leading to reduced growth rates, increased mortality, and decreased egg production. The objective of this study was to use an avian model to determine the pathogenicity of some filamentous fungal isolates from litter, feed, and fecal samples on day-old birds.  Continue reading “Pathogenicity of filamentous fungi isolated from poultry farms on gastrointestinal system of day-old chicks in Anambra State, Nigeria”

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Antimicrobial susceptibility pattern of Salmonella isolates from clinical, environmental and food sources in Lagos, Nigeria

Ajoseh, S. O., Fakorede, C. O., Abegunrin, R. O., Sodipo, C. O., Lawal-Sanni, A. O.,  Salami, W. O., and *Akinyemi, K. O.

Department of Microbiology, Lagos State University, Ojo, Lagos, Nigeria

*Correspondence to: kabiru.akinyemi@lasu.edu.ng; +2348029088676

Abstract:

 Background: Salmonellosis persists in Lagos, Nigeria, despite interventions. This study investigated antimicrobial resistance patterns of Salmonella species from clinical, food, and environmental samples in Lagos.    Methodology: From June to December 2018, 276 samples (86 blood, 80 stool, 40 hospital effluents, and 70 food) were collected for microbiological analysis. Clinical samples (blood and stool) were collected from patients in General Hospital Igando and Lagos State University Teaching Hospital Ikeja. Food samples were collected from food vendors in the urban periphery of Lagos State, and hospital effluents were collected from Gbagada General Hospital and Ojo Health Centre. The samples were processed using standard microbiological procedures.

Antimicrobial susceptibility of isolated bacteria was done using the Kirby-Bauer disk diffusion method. Continue reading “Antimicrobial susceptibility pattern of Salmonella isolates from clinical, environmental and food sources in Lagos, Nigeria”

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Bacteriological quality of Datura stramonium cocktail (‘gegemu’) and antimicrobial susceptibility of isolated bacterial pathogens

*1Onipede, J. A., 2Morka, E., 1Adeleye, O. R., and 3Onipede, O. M.

Abstract:
Background: Datura stramonium, commonly known as Jimson weed, is widely recognized in traditional medicine, and is a key ingredient in various herbal concoctions. Aside from the well-known herbal formulation, a cocktail made from Datura stramonium, known locally as ‘gegemu’, has become increasingly popular among students in our environment, raising concerns about its microbial quality. The fact that this cocktail is not commercially available raises the possibility of contamination by pathogenic microorganisms during preparation or storage, posing significant health risk. The objective of this study was to assess the microbial quality of this cocktail drink and determine the antimicrobial susceptibility of the bacteria isolates. Continue reading “Bacteriological quality of Datura stramonium cocktail (‘gegemu’) and antimicrobial susceptibility of isolated bacterial pathogens”

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Prevalence of high-risk HPV types 16 and 18 in relation to immune status and cervical cytological profile of HIV-infected women on antiretroviral therapy in northcentral Nigeria

1Ajang, A. Y., 1Ella, E. E., 1Oguntayo, A. O., 2Innocent, E., and 1Aminu, M.

1Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Nigeria
1Department of Oncology, Ahmadu Bello University Teaching Hospital, Zaria, Nigeria
2Department of Histopathology, Jos University Teaching Hospital, Jos, Nigeria
*Correspondence to: yakubuabubakar92@yahoo.com

Abstract:
Background: Human papillomavirus (HPV) is a well-established causal agent of cervical cancer, and the first group of viruses to have been acknowledged to prompt carcinogenesis. They are linked with cancers of the uterine cervix, anogenital tumours, and head and neck malignancies. Cervical cancer is by far the most common HPV-related disease, with about 99% of cervical cancer cases caused by persistent genital high-risk (HR) HPVs, especially types 16 and 18. Continue reading “Prevalence of high-risk HPV types 16 and 18 in relation to immune status and cervical cytological profile of HIV-infected women on antiretroviral therapy in northcentral Nigeria”

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Phenotypic detection of extended-spectrum β-lactamase Enterobacterales isolated from people living with HIV/AIDS in Kinshasa, Democratic Republic of the Congo

1Mbusa Vihembo, G., 1Mbundu Lukukula, C., 1Ngeleka Ngoie J., 2Indeko Lomboto, J., 2Mpoyo Munanga, L., 3Nzingula Phasi, O., 4Bayebila Menanzambi, T., 5Denis, O., 1TakaisiKikuni, N. B., 1Liesse Iyamba, J. M., and *1Mulwahali Wambale, J.

1University Reference Center of Antimicrobial Resistance Surveillance (URC-AMRS), Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo

2BOYAMBI Hospital Centre Laboratory, Barumbu, Kinshasa, Democratic Republic of Congo

3Biochemistry Laboratory, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo

4Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo

5National Reference Center for Antimicrobial Resistance in Gram-negative bacilli, CHU UCL NAMUR-Yvoir, Belgium

*Correspondence to: jose.mulwahali@unikin.ac.cd

Abstract:
Background: People living with HIV/AIDS (PLWHA) are prone to opportunistic bacterial infections caused by multidrug-resistant organisms. The aim of this study was to determine the susceptibility of Enterobacterales isolated from urine and stool samples of PLWA attending the BOYAMBI Hospital Center, Kinshasa, Democratic Republic of the Congo to commonly used antibiotics and to detect extended-spectrum β-lactamases (ESBLs) producers among the isolates. Continue reading “Phenotypic detection of extended-spectrum β-lactamase Enterobacterales isolated from people living with HIV/AIDS in Kinshasa, Democratic Republic of the Congo”

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Vaginal carriage of Streptococcus agalactiae among pregnant women in Ouagadougou, Burkina Faso

*1Ky/Ba, A., 1Ouattara, A., 2Issa, T., 3Ky, A. Y., 1Ki, C., 2Sanou, M., and 4Sanou, I.

1Bogodogo University Teaching Hospital, Ouagadougou, Burkina Faso

2Charles De Gaulle Paediatric University Teaching Hospital, Ouagadougou, Burkina Faso

3World Vision International/ Burkina Office, Ouagadougou, Burkina Faso

4Tingandogo University Teaching Hospital, Ouagadougou, Burkina Faso

*Correspondence to: absetou@yahoo.fr; +22670120520

Abstract:
Background: Group B Streptococcus (GBS) is one of the main bacteria responsible of serious neonatal infections. Neonatal transmission is very high at the end of pregnancy between the 34th and 38th week of gestation, and the systematic screening of GBS is strongly recommended in Burkina Faso. The objective of this study was to assess the prevalence of GBS carriage among pregnant women in Ouagadougou, Burkina Faso. Continue reading “Vaginal carriage of Streptococcus agalactiae among pregnant women in Ouagadougou, Burkina Faso”

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Bacteraemia at the tertiary care University Hospital Yalgado Ouedraogo: Bacterial species and their antibiotic resistance profiles

*1,2Kafando, H., 3,4Diallo, I., 5Sanou, G., 6Lompo, P., 1Ouédraogo, G. S., 4,7Sanou, I., 4,8Koeta, F., and 2,9Ouédraogo, A. S.

1Laboratory of Bacteriology-Virology, University Hospital Yalgado Ouedraogo, 03 BP 7022, Ouagadougou, Burkina Faso

2National Reference Laboratory for Antimicrobial Resistance Control (NRL-AMR), Bobo Dioulasso, Burkina Faso

3Department of Infectious Diseases, University Hospital Yalgado Ouedraogo, Ouagadougou, Burkina Faso

4Training and Research Unit in Health Science, University Joseph Ki-Zerbo, Ouagadougou, Burkina Faso

5Departement of Nephrology-Haemodialysis, University Hospital Yalgado Ouedraogo, Ouagadougou,
Burkina Faso

6Institut de Recherche en Sciences de la Sante, Clinical Research Unit of Nanoro (IRSS-URCN), Nanoro, Burkina Faso

7Department of Laboratory, University Hospital of Tengandogo, Ouagadougou, Burkina Faso

8Department of Paediatrics, University Hospital Yalgado Ouedraogo, Ouagadougou, Burkina Faso

9National Institute of Health Sciences, University Nazi Boni, Bobo Dioulasso, Burkina Faso

*Correspondence to: hervekafando7@gmail.com; +22676146695

Abstract:
Background: Bloodstream infections are serious health problem because of the significant morbidity and mortality they cause. The number of deaths increases in the presence of multidrug resistant bacteria. The aim of this study was to determine the bacteriological and resistance profiles of bacteria isolated from blood cultures. Continue reading “Bacteraemia at the tertiary care University Hospital Yalgado Ouedraogo: Bacterial species and their antibiotic resistance profiles”

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Antibiotic resistance in uropathogenic Escherichia coli strains at Brazzaville University Hospital, Congo and the therapeutic consequences

1,2Mieret, T., 1,3Ontsira Ngoyi, E. N., 1,4Aloumba, A., 1, 4Ossibi Ibara, B. R., and 1,5Odzebe, A. W. S.

1Faculty of Health Sciences, Marien Ngouabi University, Brazzaville, Congo

2National Public Health Laboratory, Brazzaville, Congo

3Bacteriology-Virology Laboratory, Brazzaville University Hospital, Congo

4Department of Infectious Diseases, Brazzaville University Hospital, Congo

5Department of Urology, Brazzaville University Hospital, Congo

*Correspondence to: tmieret@gmail.com; Tel: 00242068806913

Abstract:
Background: Urinary tract infections (UTIs) are a very frequent reason for consultations and antibiotic prescriptions in everyday practice. Excessive and inappropriate use of antibiotics is responsible for the emergence and spread of multidrug-resistant (MDR) uropathogenic bacteria. The aim of this study was to determine the frequency of isolation and antibiotic resistance of uropathogenic strains of Escherichia coli (UPEC) isolated in the bacteriology-virology laboratory of the University Hospital Centre (CHU) in Brazzaville, Congo. Continue reading “Antibiotic resistance in uropathogenic Escherichia coli strains at Brazzaville University Hospital, Congo and the therapeutic consequences”