Abstract
Antimicrobial activities of extracts of fruit bodies of Lycoperdon perlatum against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, Candida albicans and Candida glabrata were investigated. Antimicrobial components from the mushrooms were extracted using ethanol, methanol and water. The antimicrobial activities were examined by agar well diffusion method. The MIC, MBC and MFC were evaluated for each extract of the mushroom. The aqueous extract of Lycoperdon perlatum inhibited the growth of all the tested pathogenic organisms except P. aeruginosa while the methanol and ethanol extracts inhibited all the tested organisms. The phytochemical analysis revealed the presence of varying levels of bioactive compounds. Flavonoids, saponins, protein and carbohydrate were detected in all the extracts while glycosides, alkaloids and tannins were found in some. The results obtained from this study suggest that Lycoperdon perlatum has broad-spectrum of activity against microbial isolates used.
Keywords: Lycoperdon perlatum, antimicrobial, phytochemicals, well diffusion