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Prevalence of New Delhi metallo-β-lactamase gene among multi- drug-resistant Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa clinical isolates at the University College Hospital, Ibadan, Nigeria

University College Hospital, Ibadan, Nigeria

*1,2Jugah, C. C., 1,3Makanjuola, O. B., 1Ogunleye, V. O., 4Azuh, V. O., 4Kazeem, O. S.,

1,4Subulade, A. A., 4Adesanya, O. A., 4Adeniyi, A. V, and 1,3Kehinde, A. O.

   1Department of Medical Microbiology and Parasitology, College of Medicine, University of Ibadan, Nigeria

2College of Medicine, University of Ibadan, Nigeria

3University College Hospital, Ibadan, Nigeria

4Biorepository and Clinical Virology Laboratory, College of Medicine, University of Ibadan, Nigeria  *Correspondence to: christianaokiyi25@gmail.com

 

Abstract:

Background: Failure of antimicrobial therapy may give an insight into carriage of resistance genes among microbial pathogens. The ease of spread of these genes including beta-lactamase genes such as blaNDM, blaKPC, and blaOXA-48 is a major public health challenge worldwide. The study aimed to detect New Delhi metallo-β-lactamase (blaNDM) gene carriage among multidrug-resistant (MDR) Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa isolates from clinical specimens at the University College Hospital, Ibadan (UCH), Ibadan, Nigeria. Continue reading “Prevalence of New Delhi metallo-β-lactamase gene among multi- drug-resistant Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa clinical isolates at the University College Hospital, Ibadan, Nigeria”

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Molecular detection of tetracycline resistance genes in an emerging Enterobacteriaceae isolate from earthen catfish ponds in Akure, Nigeria

*Fadare, O. S., Olalekan, P., and *Salam, L. B.

Department of Biological Sciences (Microbiology Unit), Faculty of Basic and Applied Sciences, Elizade University, Wuraola Ade Ojo Avenue, P. M. B. 002, Ilara-Mokin, Ondo State, Nigeria

*Correspondence to: olalekan.fadare@elizadeuniversity.edu.ng; +2348169001041; ORCiD: 0000-0002-1749-2238 *Correspondence to: lateef.salam@elizadeuniversity.edu.ng; +234 810 245 0606; ORCID: 0000-0001-8353-534X

Abstract:

Background: Aquaculture environments are increasingly recognized as reservoirs for antimicrobial-resistant bacteria due to the routine use of antibiotics such as tetracyclines. Members of the family Enterobacteriaceae readily acquire and disseminate resistance determinants, posing risks to food safety and public health. In Nigeria, molecular data on tetracycline resistance in aquaculture systems remain limited. This study investigated the occurrence and molecular basis of tetracycline resistance among Enterobacteriaceae isolated from earthen catfish ponds in Akure, Nigeria.    Continue reading “Molecular detection of tetracycline resistance genes in an emerging Enterobacteriaceae isolate from earthen catfish ponds in Akure, Nigeria”

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Assessment of genetic diversity in Aspergillus carbonarius through random amplified polymorphic DNA (RAPD) marker analysis

1Thomas, B. T., *1Coker, M. O., 1Popoola, O. D., 1Adesetan, T. O., 1Banjo, O. A., 1Taiwo, O. S., 1Popoola, K. E., 2Thomas, A. N., 1Adegbe, G. M., and 3Owolabi, S. L.

1Department of Microbiology, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria

2Department of Animal Production, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria

3Department of Science Laboratory Technology (Microbiology Unit), Gateway Polytechnic, Saapade, Ogun State, Nigeria

*Correspondence to: olawunmi.coker@oouagoiwoye.edu.ng; +2349054006625; ORCID ID: https://orcid.org/0000–0003–0937-7755

 

Abstract:

Background: The importance of RAPD in revealing genetic diversity among ochratoxigenic strains of Aspergillus carbonarius lies in its ability to provide critical information that can support the development of effective strategies to limit ochratoxin A contamination in agricultural produce. This study therefore focused on evaluating the genetic diversity of 20 A. carbonarius strains previously isolated in an earlier investigation.    Continue reading “Assessment of genetic diversity in Aspergillus carbonarius through random amplified polymorphic DNA (RAPD) marker analysis”

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SARS-CoV-2 faecal negativity and gastrointestinal eubiosis in non-severe COVID-19 in Lagos, Nigeria

*Bamidele, T. A., Fowora, M. A., Raheem, T. Y., Ajayi, A., Adagbada, A., Yisau, J. I.,   Salau, O. Y., Shaibu, J. O., Amoo, O. S., Aiyedogbon, N., Fesobi, T. W.,          Akintunde, G., Iwalokun, B. A., Smith, S. I., and Salako, B. L. Molecular Biology and Biotechnology Department, Nigerian Institute of Medical Research, 6, Edmund Crescent, Yaba-Lagos, Nigeria

*Correspondence to: bamideletj@gmail.com; Tel: +2348038578093; ORCID: https://orcid.org/0000–0003–2155–8639

 

Abstract:

Background: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), when disseminated to the gastrointestinal tract (GIT), can cause alterations in the composition and diversity of the gastrointestinal tract microbiota. However, there is a paucity of data linking SARS-CoV-2 fecal negativity with the GI microbial balance. This study investigated the association between the GI bacterial composition and clinically defined asymptomatic, mild/moderate COVID-19 fecal-negative individuals.

Methodology: This was a cross sectional, comparative study of GI bacterial composition in COVID-19 nasopharyngeal (NP) -positive (n=7) and negative (n=5) participants at the testing facility of the Nigerian Institute of Medical Research between 6th July and 8th August 2022. Faecal samples were collected from the 12 participants. The RNA extracted from the NP-positive samples was used for RT‒qPCR detection of the nucleocapsid and open reading frame (ORF1ab) genes, while DNA from all the faecal samples was used for 16S rRNA metataxonomic analysis.      Continue reading “SARS-CoV-2 faecal negativity and gastrointestinal eubiosis in non-severe COVID-19 in Lagos, Nigeria”

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Preservative potentials and stability of bacteriocin-producing lactic acid bacteria of dairy origins

[1]Sangodare, A. O., *[2]Omotoso, I. O., 1Omotoso, O. G., and 3Samuel, T. A.  

 1Department of Science Laboratory Technology, Faculty of Pure and Applied Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria

2Department of Environmental Health Science, Faculty of Basic Medical Science, Ajayi Crowther University, Oyo, Oyo State, Nigeria

3Department of Microbiology, Faculty of Life Science, University of Ilorin, Ilorin, Nigeria *Correspondence to: omotosoifeoluwa@gmail.com; +2348068193274

 

Abstract:

 Background: Lactic acid bacteria (LAB) from dairy sources are known to produce bacteriocins with promising antimicrobial properties. The variations in their preservative effectiveness and stability under different environ- mental conditions create a gap in knowledge. This study aimed to produce bacteriocin and determine their stability properties of bacteriocin isolated from lactic acid bacteria indigenous to selected fermented diary (cheese and ‘Nunu’) within Ilorin metropolis.   Continue reading “Preservative potentials and stability of bacteriocin-producing lactic acid bacteria of dairy origins”

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Antibiofilm activity of plant lectins: Evaluating the anti-adhesion properties of Treculia africana, Moringa oleifera, and Allium sativum on clinical bacterial isolates

1Onyekwere, U. U., *2Eluu, S. C., 3Eluu, K., 4Aina, T., 5Enyigwe, J. O., 6Okoye, C. S., 2Nwankwo, U. V., 7Burba, R. A., 4Akinbiola, A. B., 8Oke, B., 9Ebem, E. C., and 10Eze, G. O.

1Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, 420110, Agulu, Anambra State, Nigeria

2Department of Biotechnology, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria

3Department of Genetics and Biotechnology, Faculty of Biological Sciences, University of Calabar, Calabar, Nigeria

4Department of Biomedical Engineering, Afe Babalola University Ado-Ekiti, KM 8.5 Afe Babalola Way, Ado-Ekiti, Nigeria

5Department of Agricultural Economics, Management and Extension, Ebonyi State University, Abakaliki, Nigeria

6Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences. David Umahi Federal University of Health Sciences, Uburu, Ebonyi State, Nigeria

7Department of Family and Consumer Science, Faculty of Agriculture, Taraba State University Jalingo, Nigeria

8Applied Microbiology Department, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria

9Department of Biotechnology, Alex Ekwueme Federal University, Ndufu-Alike Ikwo, Abakaliki, Ebonyi State, Nigeria

10Department of Scientific and Research Services, David Umahi Federal University Teaching Hospital, Uburu, Ebonyi State, Nigeria                                             *Correspondence to: eluustanleychijioke@gmail.com

 

Abstract:

Background: The increasing prevalence of biofilm-associated infections caused by multidrug-resistant organisms has intensified the search for alternative antimicrobial agents. This study investigated the antibiofilm activity of lectins isolated from Treculia africana leaves (TAL), Moringa oleifera leaves (MOL), and Allium sativum bulbs (ASL) against selected clinical bacterial isolates.  Continue reading “Antibiofilm activity of plant lectins: Evaluating the anti-adhesion properties of Treculia africana, Moringa oleifera, and Allium sativum on clinical bacterial isolates”

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Prevalence of vaginal Candida albicans among women attending Federal Medical Centre, Makurdi, Nigeria

1Ochekpe, O. S., 1Umeh, E. U., 1Amali, O., 1Ogbonna, I. O., 2Adikwu, P., and *2Agbo, A. A.

1Federal University of Agriculture, Makurdi, Benue State, Nigeria

2Federal University of Health Sciences, Otukpo, Benue State, Nigeria  *Correspondence to: ustynameh@gmail.com

 

Abstract:

Background: Candida albicans accounts for the most common type of opportunistic fungal infection affecting human heath globally, with more than a billion cases annually. The prevalence is reportedly high in resource-limited settings. The aim of this study is to determine the prevalence of vaginal C. albicans among women attending Federal Medical Centre, Makurdi, Nigeria.   Continue reading “Prevalence of vaginal Candida albicans among women attending Federal Medical Centre, Makurdi, Nigeria”

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Prevalence of pulmonary mycoses among patients with suspected pulmonary tuberculosis at the Infectious Disease Hospital, Kano, Nigeria

*Salim, A. B., and Nabila, M. I.

Department of Microbiology, Faculty of Life Sciences, Bayero University, Kano, PMB 3011, Kano, Nigeria

*Correspondence to: ahmadbsalim@gmail.com; +2348033731917

Abstract:

Background: Opportunistic fungal infections have been implicated among patients with tuberculosis (TB). Coexistence of pulmonary mycoses and pulmonary TB is of public health concern as they exhibit similar clinical manifestations among infected patients, which contributes to increased misdiagnosis and makes management difficult, especially in TB-prone regions like Kano, Nigeria. Thus, this study was aimed at determining the prevalence of pulmonary mycoses among patients with suspected pulmonary TB attending the Infectious Disease Hospital (IDH), Kano State, Nigeria.                                                Continue reading “Prevalence of pulmonary mycoses among patients with suspected pulmonary tuberculosis at the Infectious Disease Hospital, Kano, Nigeria”

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Relationship between serum viral load and stages of hepatic fibrosis in patients with chronic hepatitis C virus infections in Plateau State, Nigeria

1,2,3Maktep, Y, D., 1,2Egah, D. Z., and 1Nimzing, L.

¹Department of Medical Microbiology, Faculty of Clinical Sciences, College of Health Sciences, University of Jos, PMB 2084 Jos, Nigeria

²Department of Medical Microbiology, Jos University Teaching Hospital, Jos, Nigeria

³Department of Medical Microbiology and Parasitology, College of Health Sciences, Bingham University,  Jos Campus, Nigeria

*Correspondence to: yadangmaktep@gmail.com

Abstract

Background: Chronic hepatitis C virus (HCV) infection is a major cause of chronic liver disease worldwide and remains a significant contributor to liver-related morbidity and mortality. Hepatic fibrosis is the key determinant of long-term clinical outcomes. Although viral replication is central to HCV pathogenesis, the association between serum HCV load and the severity of hepatic fibrosis remain controversial. The objective of this study is to evaluate the relationship between serum HCV load and hepatic fibrosis severity, using Transient Elastography (FibroScan), among patients with chronic HCV infection.      Continue reading “Relationship between serum viral load and stages of hepatic fibrosis in patients with chronic hepatitis C virus infections in Plateau State, Nigeria”

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Integron-mediated multidrug resistance in uropathogenic Escherichia coli isolates from intensive care unit: Molecular evidence from Iran within a Global Surveillance Framework

Ebtehaj Pishva Independent Researcher (Allied Health Assistance), Sydney, Australia

Correspondence to: ebtehajpishva2015@gmail.com

Abstract:

Background: Integrons are genetic platforms that facilitate acquisition and expression of antimicrobial resistance gene cassettes. Their contribution to multidrug resistance (MDR) among intensive care unit (ICU) uropathogenic Escherichia coli (UPEC) is of increasing concern. This study determined the prevalence of class 1 and class 2 integrons among ICU UPEC isolates and examined their association with resistance phenotypes. Continue reading “Integron-mediated multidrug resistance in uropathogenic Escherichia coli isolates from intensive care unit: Molecular evidence from Iran within a Global Surveillance Framework”