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Burden and predictors of drug-resistant tuberculosis in Burkina Faso

[1]Sawadogo, T. L., *1Yooda, A. P., 2Kambiré, D., 3Simporé, A., 4Nagalo, Y. A., 5Diarra, A., 2Zoure, A., A., 1Diallo, A., 1Paré, S. J. E., 1Combary, A., 1Saouadogo, T., 6Sanou, G.,     7Sanou, A. M., 6Bonkoungou, I. J. O., and 2Diabouga, P. S.

1Programme National de Lutte contre la Tuberculose, Ministère de la Santé, Ouagadougou, Burkina Faso

2Institut de Recherche en Sciences de la Santé (IRSS/CNRST), LR-Maladies Infectieuses et Parasitaires (LR-MIP), Ouagadougou, Burkina Faso

3Agence Nationale Pour la Sécurité Sanitaire de l’Environnement, de l’Alimentation, du Travail et des Produits de

Santé (ANSSEAT), Ouagadougou, Burkina Faso

4Université Lédéa Bernard Ouédraogo, Unité de Formation et de Recherche en Sciences de la Santé, Ouahigouya, Burkina Faso

5Groupe de Recherche Action en Santé (GRAS)

6Université Joseph KI ZERBO/ Unité de Génomique des pathogènes One-Health, Ouagadougou, Burkina Faso

7Institut de Recherche en Sciences de la Santé (IRSS/CNRST), LR-Maladies Infectieuses et Parasitaires (LR-MIP), BOBO Dioulasso, Burkina Faso

*Correspondence to: paulyooda@gmail.com

Abstract:  

Background: In Burkina Faso, the emergence of resistance within the Mycobacterium tuberculosis complex (MTBC) represents a growing public health challenge, threatening the progress made toward tuberculosis (TB) control. This study aimed to estimate the prevalence of rifampicin- and isoniazid-resistant TB and to identify factors associated with multidrug-resistant TB (MDR-TB) among patients managed at the National Tuberculosis Control Center (CNLAT). Continue reading “Burden and predictors of drug-resistant tuberculosis in Burkina Faso”

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Rotavirus serotypes/genotypes distribution and their implications in children with diarrhoea admitted to the Charles de Gaulle University Paediatric Hospital, Ouagadougou, Burkina Faso

[1],[2]Tondé, I., [3],[4]Savadogo, H., 2,[5]Ky-Ba, A., 1Drabo, D. C., 6,7Tranchot-Diallo, J.,

1Tamboura, M., 1Kam, M., 1,2Sanou, M., 7,8Ouédraogo, A-S., 2,9Sanou, I., and 1,2Ouédraogo-Traoré, R.

1Centre Hospitalier Universitaire Pédiatrique Charles De Gaulle, Burkina Faso

2Université Joseph Ki-Zerbo, Burkina Faso

3Centre Hospitalier Universitaire Regional de Ouahigouya, Burkina Faso

4Université de Ouahigouya, Burkina Faso

5Centre Hospitalier Universitaire du District de Bogodogo, Burkina Faso

6Centre Muraz, Institut National de Santé Publique (INSP), Burkina Faso

7Université Nazi Boni, Burkina Faso

8Centre Hospitalier Universitaire Sanou-Sourô, Burkina Faso

9Centre Hospitalier Universitaire Tengandogo, Burkina Faso *Correspondence to: issa.tonde@ujkz.bf

Abstract:

Background: Diarrhoeal diseases are a source of public health problems in low-income countries such as Burkina Faso, where the aetiology of rotavirus plays an important role. This led to the introduction of surveillance since the introduction of the rotavirus vaccine. The aim of this study is to take stock of rotavirus diarrhoea since the introduction of the vaccine.     Continue reading “Rotavirus serotypes/genotypes distribution and their implications in children with diarrhoea admitted to the Charles de Gaulle University Paediatric Hospital, Ouagadougou, Burkina Faso”

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Bacteriological profile of Staphylococcus aureus isolates at the Bogodogo University Hospital Center, Ouagadougou, Burkina Faso

[1],[2]*Condé, M. S., [3],[4]Ky/Ba, A., 3,5Tondé, I., 4Savadogo, S. M., 4Ouattara, A., 4Yourgbarré, J., 4Ouédraogo, Y., 6Nagalo, Y. Y. A. A., 3Sanou, I., and 6,7Ouédraogo,A.

1Department of Biomedical Analysis Laboratory, Ignace Deen University Hospital, Conakry, Guinea

2Faculty of Health Sciences, Gamal Abdel Nasser University, Conakry, Guinea

3Laboratory of Bacteriology-Virology, UFR SDS, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso

4Department of Biomedical Analysis Laboratory, Bogodogo University Hospital, Ouagadougou, Burkina Faso

5Department of Biomedical Analysis Laboratory, Charles de Gaulle Pediatric University Hospital, Ouagadougou, Burkina Faso

6Laboratory of Emerging and Re-emerging Pathogens (LaPathER), Nazi BONI University, Bobo-Dioulasso 01 PO 676, Burkina Faso

7Bacteriology and Virology Service, Laboratory Department, Sourô Sanou University Hospital,  Bobo-Dioulasso 01 PO 676, Burkina Faso *Correspondence to: mamadisaran93@gmail.com; Tel: (226) 64645543

Abstract:

Background: Staphylococcus aureus is a common major human pathogen involved in a wide variety of infections. The objective of this study is to investigate the bacteriological profile of S. aureus at the Bogodogo University

Hospital Center, Ouagadougou, Burkina Faso.     Continue reading “Bacteriological profile of Staphylococcus aureus isolates at the Bogodogo University Hospital Center, Ouagadougou, Burkina Faso”

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Antibiotic susceptibility of bacteria isolated from suppurative acute otitis media in children in Ouagadougou, Burkina Faso  

[1],3Tondé, I., [2]Guiguemdé, K. T., *[3],[4]Condé, M. S., 2Savadogo, P. M., 2,3Sanogo, Z., 1,3Tapsoba, A., 3Tamboura, M., 2,3Soma, W., 1Béré, P. L., 3Somé, C. K., 1Ky/Ba, A., 1Sanou, I., and [5]Gyebré, Y. M. C.

1Laboratory of Bacteriology-Virology, UFR SDS, Joseph Ki-Zerbo Université, Ouagadougou, Burkina Faso

2Laboratory of Parasitology-Mycology, UFR SDS, Joseph Ki-Zerbo Université, Ouagadougou, Burkina Faso

3Department of Biomedical Analysis Laboratory, Charles de Gaulle Pediatric University Hospital, Ouagadougou, Burkina Faso

4Department of Biomedical Analysis Laboratory, Ignace Deen University Hospital, Conakry Guinea

5Department of Otolaryngology, Yalgado Ouédraogo University Hospital, Ouagadougou, Burkina Faso *Correspondence to: mamadisaran93@gmail.com; Tel: (226) 64645543

Abstract:

Background: Acute otitis media (AOM) is one of the most common bacterial infections in children. The emergence of antimicrobial resistance (AMR) poses a threat to the management of these infections. The objective of this study was to identify and describe the sensitivity profile of bacterial agents isolated in AOM to commonly used antibiotics in the city of Ouagadougou, Burkina Faso.                            Continue reading “Antibiotic susceptibility of bacteria isolated from suppurative acute otitis media in children in Ouagadougou, Burkina Faso  “

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Antibiotic susceptibility of uropathogenic Escherichia coli isolates in a hospital setting in Ouagadougou, Burkina Faso: A twelve-year retrospective analysis

[1]*Kafando, H., 1Ouattara, M., 1Kienou, M., 1Coulidiaty, Y. D., 1Ouattara, K., 1Ouédraogo, R., 1Sawadogo, M., 1Guira, C., 3,4Ouédraogo, A. S., [2],5Sanou, I., and 1,2Sangaré, L.

 

1Department of Bacteriology and Virology, Yalgado Ouedraogo University Hospital, 03 BP 7022, Ouagadougou 03, Burkina Faso

2Training and Research Unit in Health Science, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso

3National Reference Laboratory for the Control of Antimicrobial Resistance (NRL-AMR), Bobo Dioulasso, Burkina Faso

4National Institute of Health Sciences, NAZI BONI University, Bobo Dioulasso, Burkina Faso

5Laboratory Department, Tengandogo University Hospital, Ouagadougou, Burkina Faso *Correspondence to: hervekafando7@gmail.com; 0022676146695

 

Abstract:

 Background: Escherichia coli is the main bacterium responsible for uncomplicated urinary tract infections (UTI). The increasing frequency of antibiotic resistance in E. coli isolates from UTI poses concern in their therapeutic management. The aim of this study is to describe the current antibiotic resistance profile of E. coli clinical isolates at the Yalgado Ouedraogo University Hospital of Ouagadougou (CHUYO), Burkina Faso, with a view to revising the treatment protocols for bacterial UTI. Continue reading “Antibiotic susceptibility of uropathogenic Escherichia coli isolates in a hospital setting in Ouagadougou, Burkina Faso: A twelve-year retrospective analysis”

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Prevalence of COVID-19 at the Wahgnion-Gold mining site in Burkina Faso and use of RT-PCR initial cycle threshold to monitor the dynamics of SARS-CoV-2 load

+1Cissé, A., +*1,2Lingani, M., 1Tao, M., 1Nana, S., 1Kaboré, B., 3Eric D. A. S., 4Nassouri, A., 1Diagbouga, S., 5Bamba, S., and 1Tarnagda, Z.

1National Influenza Reference Laboratory, Unité des Maladies à Potentiel Epidémique, Maladies Émergentes et

Zoonoses, Institut de Recherche en Sciences de la Santé, Burkina Faso

2Unité de Recherche Clinique de Nanoro, Institut de Recherche en Sciences de la Santé, Nanoro, Burkina Faso

3Teranga Gold Corporation, Ouagadougou, Burkina Faso

4Wahgnion Gold Operation, Ouagadougou, Burkina Faso

5Institut Supérieur des Sciences de la Santé, Université Nazi BONI, Bobo-Dioulasso, Burkina Faso

*Correspondence to: lingani10@yahoo.fr; +joint first authors

 

Abstract:

 Background: To control the spread of coronavirus disease-19 (COVID-19) caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), it is necessary to adequately identify and isolate infectious patients particularly at the work place. Real time polymerase chain reaction (RT-PCR) assay is the recommended confirmatory method for the diagnosis of SARS-CoV-2 infection. The aim of this study was to determine the prevalence of SARSCoV-2 infection in Burkina Faso and to use the initial cycle threshold (Ct) values of RT-PCR as a tool to monitor the dynamics of the viral load.

Methodology: Between September 2021 and February 2022, oropharyngeal and/or nasopharyngeal swab samples of consecutively selected COVID-19 symptomatic and apparently healthy workers from the Wahgnion mining site in the South-western Burkina Faso who consented to the study were collected according to the two weeks shift program and tested for SARS-CoV-2 using RT-PCR assay. Patients positive for the virus were followed-up weekly until tests were negative. Association of the initial RT-PCR Ct values with disease duration was assessed by adjusted linear regression approach. Two-sided p value < 0.05 was considered statistically significant. Continue reading “Prevalence of COVID-19 at the Wahgnion-Gold mining site in Burkina Faso and use of RT-PCR initial cycle threshold to monitor the dynamics of SARS-CoV-2 load”

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Epidemiology of Dengue in patients with febrile syndrome at Saint Camille Hospital, Ouagadougou, Burkina Faso from 2020 to 2021

1,2,3Bello, S. O. T., 4Houkpevi, A., 4Zackari, S., 1,3Tapsoba, A. S. A., *1,3,5Zoure, A. A., 6Ilboudo, P. D., 1,3Ouattara, A. K., 1Traore, L., 3Belemgnegre, M., 1,3,4Zohoncon, T. M.,  2,5Diabate, A., and 1,3,4Simpore, J.

1Laboratory of Molecular Biology and Genetics (CERBA/LABIOGENE), UFR/SVT, Joseph KI-ZERBO University, BP 7021 Ouagadougou 03, Burkina Faso

2African Center of Excellence in Biotechnological Innovations for the Elimination of Vector-borne Diseases

(CEA/ITECH-MTV), Nazi BONI University 01BP, 1091 Bobo-Dioulasso 01, Burkina Faso

3Saint Camille Hospital of Ouagadougou (HOSCO), 01 BP 444 Ouagadougou 01, Burkina Faso

4Faculty of Health Sciences, Saint Thomas Aquinas University (USTA), 06 BP 10212, Ouagadougou 06, Burkina Faso

5Institute for Research in Health Sciences (IRSS/CNRST)/Muraz Center, BP 390, Bobo-Dioulasso, Burkina Faso

6University of Fada N’Gourma, Fada N’Gourma, Burkina Faso

*Correspondence to: abdouazaque@gmail.com; Tel: 00 226 50 34 48 01; https://orcid.org/0000–0002–9423–024X

 

Abstract:

Background: Dengue is still a public health problem in tropical countries. This disease, which had almost disappeared in some areas of the world, has become re-emergent in certain parts of the world including Africa. The aim of this study is to determine the seroprevalence and evolution of Dengue virus (DENV) infection from 2020 to 2021 at the Hospital Saint Camille de Ouagadougou (HOSCO), Burkina Faso. Continue reading “Epidemiology of Dengue in patients with febrile syndrome at Saint Camille Hospital, Ouagadougou, Burkina Faso from 2020 to 2021”

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Faecal carriage of extended-spectrum beta-lactamase-producing Enterobacteriaceae in healthy volunteers and hospitalized patients in Ouagadougou, Burkina Faso: prevalence, resistance profile, and associated risk factors

[1],[2]Soré, S., 3Sanou, S., 3Sawadogo, Y., 1Béogo, S., 3Dakouo, S. N. P.,                    4Djamalladine, M. D., 1lboudo K. S., 2Ouoba, B., ³Zoungrana, J.,                               ³Poda, A., 2Ouédraogo, A. S., and 1,2Sanou

1Tengandogo University Hospital, Ouagadougou, Burkina Faso

2Saint Thomas d’Aquin University, Doctoral School of Science, Health and Technology, Burkina Faso         

3Souro Sanou University Hospital, Bobo-Dioulasso, Burkina Faso                                         

4National Higher Institute of Science and Technology of Abeche, Abeche, Chad        

*Correspondence to: soulsore11@yahoo.fr; (00 226) 72 04 29 15

Abstract:

Background: Extended spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) are a serious challenge to patients’ treatment. The aim of this study is to determine the prevalence of ESBL-PE, investigate the associated resistance, and analyze the associated risk factors for acquisition of ESBL-PE.

Methodology: A cross-sectional study was conducted on healthy volunteers and inpatients. After obtaining informed consent, rectal swabs were collected from each participant for isolation of Enterobacteriaceae on Hektoen enteric agar containing 4µg/L cefotaxime. The Enterobacteriaceae isolates were identified using biochemical tests and ESBL production was confirmed by the double-disc synergy test of amoxicillin and clavulanic acid. Antibiotic susceptibility test of each isolate was done by the disc diffusion method and interpreted using the recommendations of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) clinical breakpoints version 5.0. Continue reading “Faecal carriage of extended-spectrum beta-lactamase-producing Enterobacteriaceae in healthy volunteers and hospitalized patients in Ouagadougou, Burkina Faso: prevalence, resistance profile, and associated risk factors”

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Prevalence of Escherichia coli virulence genes in patients with diarrhoea in Ouagadougou, Burkina Faso

N. S. Somda, O. J. I. Bonkoungou, C. Zongo, D. S. Kpoda, F. Tapsoba, I. H. N. Bassolé, Y. Traoré, A. Savadogo

 

Abstract

Objective: Diarrhoeagenic E. coli (DEC) strains are important causes of diarrhoea in the developing world and, to a lesser extent, inthe developed world. In this study, we investigated the prevalence of the virulence genes specific for five major pathogroups of diarrheagenic Escherichia coli (DEC) in primary cultures from diarrhoeagenic patients in Burkina Faso.
Methodology: From September 2016 to Mars 2017, a total of 211 faecal samples from diarrhoeagenic patients from urban hospitals of Ouagadou, Burkina Faso have been analysed. A 16-plex PCR was used to detect simultaneously, the five major DEC pathotypes (enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), Shiga toxin-producing E. coli (STEC), enteroaggregative E. coli (EAEC) and enteroinvasive E. coli (EIEC)).
Results: At least one diarrhoeagenic E. Coli pathotype was detected in 31 samples (14.7%) in children and adults with diarrhoea. EAEC was the most common pathotype detected 9.5% (20/211), followed by EIEC2.4% (05/211) and STEC 0.5% (01/211). More than one DEC pathotype were detected in 2.4% (05/211) patients. EPEC and ETEC were not detected in single infection but in co-infection with others pathotypes.
Conclusion: DEC, especially enteroaggregative, may be important responsible of diarrhoeas in Burkina Faso from all ages patient.

Key Words: Diarrhoeagenic Escherichia coli, 16-plex PCR, Burkina Faso, human diarrhoeas stool.

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Prevalence of Escherichia coli virulence genes in patients with diarrhoea in Ouagadougou, Burkina Faso

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Antimicrobial susceptibility of extended-spectrum beta-lactamase producing Enterobacteriaceae causing urinary tract infections in Ouagadougou, Burkina Faso

D.S. Kpoda, N Guessennd, N.S. Somda, A Ajayi, J.I. Bonkoungou, F Konan, M.B. Ouattara, M Somda, J Simpore, R Ouedraogo, K.M. Drabo, L Sangare, M Dosso, A.S. Traore

 

Abstract

Objective: To determine the frequency of extended-spectrum beta lactamase producing Enterobacteriaceae (ESBL) and other antibioticsresistant bacteria in urinary tract isolates.

Study Design: prospective and experimental study.

Methodology: Place and duration of study :YalgadoOuedraogo University Hospital Center, Charles De Gaulle Pediatric Hospital Center, Saint Camille Hospital and National Public Health Laboratory, Ouagadougou, from November 2014 to October 2015.

AllEnterobacteriaceaestrains isolated from urinary samples of patients were identifiedusing API 20E chemical gallery (BioMerieux, France). All strains were subjected to an array of 14 antibiotics to study their drug susceptibility by using Kirby- Baeurdisk diffusion method. Detection of ESBL was carried out by double disk diffusion technique. Statistical analysis was performed by Microsoft Excel and Anova one-way GrapPad Prism version 5.01. Chi-square (χ2) test was used to determine significance. A p˂ 0.05was considered to be statistically significant.

Results: A total of 324 isolates of Enterobacteriaceae were identified during the study period, including211(65%) E. coli, 75 (23%)Klebsiellaspp., 18 (6%) Enterobacter spp., 11 (3%)Proteus spp., 5 (2%) Citrobacter spp., Serratia spp. 3 (1%).All the clinical isolates were susceptible to imipenem. Resistance to amikacinwas 14% (45/324); gentamicin 54% (175/324); tobramycin 58% (187/324); nalidixic acid 72% (234/324),ciprofloxacin 63% (204/324) and to cotrimoxazole 83% (269/324).The overall rate of the EBSL producing strains was 35% (114/324). Their susceptibility to antibiotics was (imipenem,amikacin, cefoxitin and fosfomycin) 100% (114/114), 93% (106/114), 74% (84/114) and 84% (96/114) respectively. ESBL positivity within individual organism group was highest inEscherichia coli 64% (73/324) followed byKlebsiellaspp. 28% (32/324), Enterobacterspp. 3% (4/324), Proteus spp. and Citrobacterspp. 2% (2/324).

Conclusion: The results showeda high frequency of ESBL producing Enterobacteriaceae, especially Escherichia coli and Klebsiellaspp. The data points to theneed of routine detection and surveillance of ESBL producing bacteria in Burkina Faso.

Keywords: Antimicrobial susceptibility, Enterobacteriaceae, Urine, Burkina Faso

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Antimicrobial susceptibility of extended-spectrum beta-lactamase producing Enterobacteriaceae causing urinary tract infections in Ouagadougou, Burkina Faso